Investigating the effect of alcohol exposure on human cortical development using a 3D in vitro model

使用 3D 体外模型研究酒精暴露对人类皮质发育的影响

基本信息

批准号：
10811223
负责人：
Ray Yueh Ku
金额：
$ 1.47万
依托单位：
CHILDREN'S RESEARCH INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-03-17 至 2024-11-11
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10811223
关键词：
3-Dimensional Acute Administrative Supplement Alcohol consumption Alcohols Animal Model Biomedical Research Bioreactors Brain CRISPR/Cas technology Cell Death Cell Line Cell Proliferation Cells Cerebrum Chronic Clinical Trials Cues Data Development Dose Embryonic Development Ethanol Exposure to Fellowship Fetal Alcohol Exposure Fetal Alcohol Spectrum Disorder Fetal Development Funding Future Grant Human Hyperoxia Immunohistochemistry In Vitro Knowledge Manuscripts Mediating Modeling Modernization Monitor Morphology Mus Neuroglia Neuronal Differentiation Neurons Parents Patients Population Predisposition Pregnancy Preparation Primates Process Proliferating RNA-Binding Proteins Radial Research Resources Role Stress Structure System Testing Time U-Series Cooperative Agreements United States National Institutes of Health Up-Regulation alcohol consumption during pregnancy alcohol effect alcohol exposure cell injury cell type cognitive function cost falls genome editing human data in vitro Model induced pluripotent stem cell insight migration mouse model nerve stem cell neural neuroprotection novel overexpression parent project prenatal influence progenitor response stem cell proliferation stem cells subventricular zone timeline transcriptome sequencing transcriptomics

项目摘要

Project summary This project is prepared in response to PA-20-272 (Administrative Supplements to Existing NIH Grants and Cooperative Agreements (Parent Admin Supp Clinical Trial Optional). The PI is in no cost extension period of her F32 fellowship, partially supported by funds carried forward from previous years. The PI successfully generated human iPSC-derived cortical spheroids in defined, differentiation-promoting culture medium and investigated the effect of ethanol on early brain development. We have assessed the effects of ethanol on cortical development by monitoring cell death, morphological change, cellular proliferation, and the progenitor cell population with an emphasis on outer radial glia-like cells in the spheroids at cellular level. This proposed administrative supplement will allow the PI completing analysis of acquired RNA sequencing data in preparation of future manuscript. This administrative supplement will also provide the necessary resources allowing an orderly completion of the parent project.

项目概要该项目是根据 PA-20-272（现有 NIH 拨款的行政补充和合作协议（家长管理支持临床试验可选）。 PI 处于免费延长期内她的 F32 奖学金，部分由前几年结转的资金支持。 PI成功在确定的、促进分化的培养基中生成人类 iPSC 衍生的皮质球体，并研究乙醇对早期大脑发育的影响。我们评估了乙醇对通过监测细胞死亡、形态变化、细胞增殖和祖细胞群，重点是细胞水平上球体中的外放射状神经胶质细胞。拟议的行政补充将允许 PI 完成对获得的 RNA 测序的分析准备未来手稿的数据。该行政补充还将提供必要的资源允许有序完成父项目。