Quorum sensing in Pseudomonas aeruginosa - Equipment Supplement

铜绿假单胞菌中的群体感应 - 设备补充

• 批准号: 10793892
• 负责人: Everett P Greenberg
• 金额: $ 5.7万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10793892
• 关键词: Biological; Biological Phenomena; Biotechnology; Cell Communication; Cell Density; Cells; Chronic; Communication; Complex; Cooperative Behavior; Costs and Benefits; Cystic Fibrosis; Development; Elements; Equipment; Gene Expression; Generations; Genetic Diseases; Human; In Vitro; Infection; Knowledge; Learning; Lung infections; Molecular; Molecular Genetics; Persons; Play; Population; Population Biology; Pseudomonas aeruginosa; Research; Role; Signal Transduction; Specificity; Study models; System; Techniques; Therapeutic; Virulence; Work; antimicrobial; biological research; genetic approach; homoserine lactone; human pathogen; inhibitor; interest; member; microbial community; novel; pathogen; pressure; quorum sensing

PROJECT SUMMARY Quorum sensing is a form of cell-cell communication that allows members of bacterial populations to coordinate cooperative activities in a cell density-dependent fashion. Quorum sensing has been shown to play a significant role in the virulence of Pseudomonas aeruginosa and other pathogens, and P. aeruginosa has become a model for studies of basic biological principles of quorum sensing, communication, and cooperation in general. Our research on P. aeruginosa acyl-homoserine lactone quorum sensing is fundamental to efforts aimed at manipulating quorum sensing during infections, in complex microbial communities, and for biotechnological applications. We have been, and will continue to be, interested in basic mechanisms of quorum sensing, the selective pressures favoring quorum-sensing control of gene expression, and the costs and benefits of quorum sensing in P. aeruginosa. Quorum sensing functions to control and coordinate cooperative behaviors. It is clear that cooperativity is an evolved biological phenomenon, but there is considerable controversy about the selective forces allowing stable cooperativity. What are the costs and benefits of cooperativity, and what are the possible advantages to controlling cooperativity by quorum sensing? In the past five years we have made enormous advances in understanding at a molecular level how cooperation is stabilized in P. aeruginosa and how we can destabilize it. These advances are important to the field of population biology and to our understanding of the roles P. aeruginosa quorum sensing plays in certain infections. Our continued research will use molecular genetic approaches to investigate the quorum sensing control of gene expression, and we will continue to introduce new techniques and concepts into what has become a vibrant field of microbiological research. Specifically we will ask questions about the determinants of signal specificity and the potential for bacterial species to eavesdrop on each other. We will attempt to define the determinants of signal generation and reception selectivity. We want to better understand the role of quorum sensing during human infections with our initial emphasis on the chronic lung infections that plague people with the genetic disease cystic fibrosis. We have recently learned that one of the least understood components of P. aeruginosa quorum sensing circuitry, RhlR is a key quorum-sensing element in P. aeruginosa. Our recent work has allowed us to overcome the major obstacle in learning about RhlR, an inability to study it in vitro. The long term vision of this work is to understand how and why quorum sensing circuits are often layered, to understand how the diversity of quorum sensing systems has evolved to its current state, and to attain a conceptual framework to explain how communication and cooperative activities are wired to provide stability of bacterial group activities and how we might be able to use the knowledge to manipulate bacterial populations.

项目摘要 法定人数传感是一种细胞 - 细胞通信的形式，可使细菌种群成员得以实现 以细胞密度依赖性方式协调合作活动。已显示法定人物的感应播放 铜绿假单胞菌和其他病原体的毒力中的重要作用，以及铜绿假单胞菌具有 成为研究群体感应，交流和合作基本生物学原理的模型 一般来说。我们关于铜绿假单胞菌酰基 - 耶洛舍碱内酯群体的研究是努力的基础 旨在操纵在感染，复杂的微生物群落和 生物技术应用。我们已经并且将继续是对的，对 群体传感，有利于基因表达的法定感应控制的选择性压力，成本 铜绿假单胞菌中法定感测的好处。群体传感功能以控制和坐标 合作行为。显然，协作性是一种进化的生物学现象，但是有 关于选择性力量允许稳定合作的争议。费用是多少？ 合作的好处，通过法定人心的感觉控​​制合作的可能优势是什么？ 在过去的五年中 铜绿假单胞菌稳定合作，以及我们如何破坏它的稳定。这些进步对 人口生物学领域以及我们对角色的理解。 感染。我们的持续研究将使用分子遗传学方法来研究法定人数 控制基因表达，我们将继续将新技术和概念引入 成为微生物研究的充满活力的领域。具体来说，我们将询问有关决定因素的问题 信号特异性和细菌物种相互窃听的潜力。我们将尝试定义 信号产生和接收选择性的决定因素。我们想更好地了解 在人类感染期间的法定人物感测，我们最初强调了瘟疫的慢性肺部感染 患有遗传疾病囊性纤维化的人。我们最近了解到，最了解的是 铜绿假单胞菌群体传感电路的组件，RHLR是P. 铜绿。我们最近的工作使我们能够克服学习RHLR的主要障碍 无法在体外研究。这项工作的长期视野是了解如何以及为何构想感应 电路通常是分层的，以了解法定感应系统的多样性如何发展为 当前状态并获得一个概念框架来解释如何交流和合作活动 有线以提供细菌小组活动的稳定性，以及我们如何使用知识 操纵细菌种群。