Extracellular Potassium and Astrocytic Kir4.1 Modulate Interneuron Activity in the Cerebral Cortex

细胞外钾和星形胶质细胞 Kir4.1 调节大脑皮层的中间神经元活动

基本信息

批准号：
10784058
负责人：
Moritz Armbruster
金额：
$ 8.79万
依托单位：
TUFTS UNIVERSITY BOSTON
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-05-15 至 2027-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10784058
关键词：
Action Potentials Affect Area Astrocytes Automobile Driving Award Biochemical Biological Assay Biology Brain Buffers Cerebral cortex Characteristics Data Data Analyses Dependence Development Electrophysiology (science)Epilepsy Epileptogenesis First Generation College Students Frequencies Funding Future Hyperactivity Image In Vitro Interneurons Investigation Latina Measures Mediating Mentors Mentorship Modeling Mutation National Research Service Awards Nervous System Neurons Neurosciences Neurosciences Research Neurotransmitters Output Parvalbumins Pathologic Pathway interactions Physiological Potassium Qualifying Research Research Personnel Role Runaway Seizures Shapes Signal Transduction Site Sleep Wake Cycle Source Statistical Data Interpretation Structure Synapses Testing Time Training Underrepresented Populations Universities Work disadvantaged background doctoral student economic disparity experience extracellular fluorescence imaging gamma-Aminobutyric Acid hippocampal pyramidal neuron imaging approach imaging modality in vitro Model insight interest inward rectifier potassium channel live cell imaging member nervous system disorder neuronal excitability neurotransmission neurotransmitter release novel overexpression pre-doctoral presynaptic research faculty spreading depression synaptic function tool uptake voltage

项目摘要

PROJECT SUMMARY This application is for a diversity supplement to the R01 NS127819-01 “EXTRACELLULAR POTASSIUM AND ASTROCYTIC KIR4.1 MODULATE INTERNEURON ACTIVTY IN THE CEREBRAL CORTEX” of Dr. Moritz Armbruster at Tufts University. The candidate is Reyna Gariepy, a pre-doctoral student in the lab of Dr. Armbruster. Reyna will take tools currently in use in the lab and use them to pursue her interests to investigate how GABA dynamics are shaped by astrocytes. Reyna qualifies as a diversity candidate as a Latina, first generation college student, and her economically disadvantaged background. Therefore, she is a member of multiple underrepresented groups. This supplement will fund Reyna for 3 years of her graduate thesis work. Funding for Y3 will be contingent on submission of an NRSA application during Y2. During this time Reyna will receive training in cellular neuroscience, glial biology, epilepsy and more. She will gain expertise in biochemical assays, astrocytes and neuronal electrophysiology, iGABASnFR imaging, and data and statistical analysis. This training will guide her towards an NRSA application and future as a leader in neuroscience research. Ms. Gariepy is devoted to academic research with a focus on epileptogenesis. Dr. Armbruster, is a junior research faculty in the Neuroscience Department at Tufts University who has extensive experience in astrocyte-neuron interactions and utilizing live-cell imaging approaches. To increase Reyna’s opportunities for development, we have included Dr. Chris Dulla as a senior mentor. Dr. Dulla has a great track record of mentorship, including the 2018 Landis Award for Outstanding Mentorship. The project laid out here will give Ms. Gariepy excellent and rigorous training in GABA neurotransmission, neuron/glial interactions, and cutting- edge imaging modalities. Dr. Armbruster, and Ms. Gariepy have developed a structured, rigorous, and customized mentorship and training plan. The funding offered through this supplement will give Reyna a clear path toward an exciting future as an up and coming researcher in the field of epilepsy and GABA neurotransmission. The activities laid out and the enrichment opportunities will be critical to Ms. Gariepy’s development and future as a successful independent investigator.

项目概要本申请是对 R01 NS127819-01“细胞外钾和星形胶质细胞 KIR4.1 调节大脑皮层的中间神经元活动”，Moritz 博士塔夫茨大学的 Armbruster 候选人是 Reyna Gariepy，她是 Dr. Armbruster 实验室的博士生。阿姆布鲁斯特（Reyna）将利用实验室目前使用的工具来追求她的兴趣进行调查。首先，星形胶质细胞如何塑造 GABA 动态？一代大学生，加上她的经济困难背景，因此，她是其中的一员。该补助将资助雷娜 3 年的研究生论文工作。第三学年的资助将取决于第二学年期间提交的 NRSA 申请。在此期间，Reyna 将提供资助。接受细胞神经科学、神经胶质生物学、癫痫等方面的培训，她将获得这些领域的专业知识。生化测定、星形胶质细胞和神经元电生理学、iGABASnFR 成像以及数据和统计这次培训将引导她走向 NRSA 应用并成为神经科学领域的领导者。 Gariepy 女士致力于癫痫发生的学术研究。塔夫茨大学神经科学系的初级研究人员，在以下方面拥有丰富的经验：星形胶质细胞-神经元相互作用并利用活细胞成像方法增加 Reyna 的机会。在开发过程中，我们聘请了 Chris Dulla 博士作为高级导师。Dulla 博士在以下领域有着出色的业绩记录。指导，包括 2018 年兰迪斯杰出指导奖。这里列出的项目将授予。 Gariepy 女士在 GABA 神经传递、神经元/神经胶质相互作用和切割方面接受过出色而严格的培训 Armbruster 博士和 Gariepy 女士开发了一种结构化、严格且可靠的边缘成像模式。通过此补充提供的定制指导和培训计划将为 Reyna 提供明确的指导。作为癫痫和 GABA 领域的后起之秀研究员，迈向激动人心的未来之路所安排的活动和丰富机会对加里皮女士来说至关重要。作为一名成功的独立调查员的发展和未来。