Transgenic and Gene Targeting Core

转基因和基因靶向核心

• 批准号: 7677656
• 负责人: Peter J. Koch
• 金额: $ 14.68万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-28 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7677656
• 关键词: Animal Model; Animals; Biological Preservation; Biology; Biomedical Research; Colorado; Consultations; Derivation procedure; Development; Equipment; Funding; Gene Targeting; Generations; Genetically Engineered Mouse; Goals; Human; Inherited; Injection of therapeutic agent; Knock-out; Knockout Mice; Laboratories; Modeling; Molecular Analysis; Mus; Price; Procedures; Process; Production; Productivity; Reagent; Recombinants; Research; Research Personnel; Research Project Grants; Services; Skin; Skin Cancer; Time; Transgenic Mice; Transgenic Organisms; Universities; appendage; base; cost; design; embryonic stem cell; experience; member; mouse model; research study; skills; skin disorder; tool; vector

Genetically engineered mice have become an essential tool in biomedical research. They are crucial tools for studying development of the skin and its appendages. In addition, these mouse models have become indispensible for creating models of human skin disorders, including inherited and aquired skin disorders and skin cancer. Generating transgenic or knockout mice requires specialized skills and expensive equipment. Consequently, very few laboratories have the ability to generate these valuable research models. The Transgenic and Gene Targeting (TGT) Core at the University of Colorado Denver offers a comprehensive set of services which enables investigators to generate genetically engineered mice. These services include the generation of transgenic mice via pronuclear injections, and gene targeting experiments that include the entire process from generating recombinant ES cells to the production of chimeric mice. All that is required from the investigators to initiate these experiments is a transgenic vector or gene targeting vector. Furthermore, our core offers extensive consultation services to help investigators with the development of a research plan, including vector design, animal design, and the analysis of genetically engineered mice. 23 investigators of the Skin Disease Research Core Center (UCD-SDRC) are planning to use our core services to generate mouse models to study skin biology or skin diseases. Core Center support for our core will provide these investigators with subsidized access to the full set of our services, and thus facilitate their research.

基因工程的小鼠已成为生物医学研究的重要工具。它们是至关重要的工具 研究皮肤的发育及其附属物。另外，这些鼠标模型已成为 不可或缺，无法创建人类皮肤疾病的模型，包括遗传和水生皮肤疾病和 皮肤癌。产生转基因或淘汰小鼠需要专业技能和昂贵的设备。 因此，很少有实验室能够生成这些有价值的研究模型。转基因 科罗拉多大学丹佛分校的基因靶向（TGT）核心提供了全面的服务 这使研究人员能够生成基因工程的小鼠。这些服务包括一代 转基因小鼠通过前核注射和基因靶向实验，其中包括整个过程 生成重组ES细胞以产生嵌合小鼠的产生。调查人员需要的一切 启动这些实验是转基因载体或靶向载体的基因。此外，我们的核心提供广泛的 咨询服务，以帮助调查人员制定研究计划，包括向量设计， 动物设计以及基因工程小鼠的分析。 23皮肤病研究的研究者 核心中心（UCD-SDRC）计划使用我们的核心服务来生成鼠标模型来研究皮肤生物学 或皮肤疾病。对我们核心的核心中心支持将为这些调查人员提供对核心的补贴访问 我们的全套服务，从而促进他们的研究。