Identification of the 1-methyladenine receptor

1-甲基腺嘌呤受体的鉴定

• 批准号: 7317815
• 负责人: Sean P Place
• 金额: $ 4.96万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7317815
• 关键词: 1-methyladenine; 1-methyladenine receptor; Antisense Oligonucleotides; Binding; Biochemical; Biological Models; Cell Cycle; Competence; Complementary DNA; Fertilization; GTP-Binding Proteins; Goals; Hormone Receptor; Hormones; Knowledge; Laboratories; Link; Mammals; Molecular; Oocytes; Process; Property; Proteins; Research Design; Signal Pathway; Signal Transduction; Starfish; System; Testing; Work; analog; base; concept; design; expression cloning; novel strategies; oocyte maturation; prevent; receptor; research study

The goal of the proposed study is to identify and characterize the oocyte maturation hormone receptor in the starfish, Patina miniata. This model system offers signficant advantages over others and some aspects may be shared with mammalian oocytes. The proposed work is designed to identify and characterize this receptor using unique homology based and functional expression cloning based approaches in tandem to identify and clone candidate cDNA(s) encoding the receptor for the maturation-inducing hormone, 1-methyladenine (1- MA). Successful identification of the 1-MA receptor using these novel strategies would represent the first definitive description of a maturation-inducing hormone receptor in any species and provide proof of concept for the strategy to be used for the identification of maturation hormone receptors in other species, including mammals. Several candidate cDNAs already have been isolated; these and additional candidates will be subjected to further functional studies to confirm identity and tests for necessity in oocyte maturation will be conducted. Eventually, studies will be conducted that are designed to elucidate the details of the molecular mechanism of signal transduction during oocyte maturation.

拟议研究的目标是识别和表征卵母细胞成熟激素受体 海星，铜绿。该模型系统比其他模型系统具有显着的优势，并且某些方面可能会 与哺乳动物卵母细胞共享。拟议的工作旨在识别和表征该受体 使用独特的基于同源性和基于功能表达克隆的方法串联来识别和 克隆候选 cDNA，编码成熟诱导激素 1-甲基腺嘌呤 (1- 马）。使用这些新策略成功鉴定 1-MA 受体将代表第一个 对任何物种中成熟诱导激素受体的明确描述并提供概念证明 用于鉴定其他物种的成熟激素受体的策略，包括 哺乳动物。一些候选 cDNA 已经被分离出来；这些和其他候选人将 将进行进一步的功能研究以确认身份并测试卵母细胞成熟的必要性 实施。最终，将进行旨在阐明分子细节的研究 卵母细胞成熟过程中的信号转导机制。

项目成果

Transcriptome profiles link environmental variation and physiological response of Mytilus californianus between Pacific tides.

转录组图谱将太平洋潮汐之间的环境变化和加州贻贝的生理反应联系起来。

• DOI: 10.1111/j.1365-2435.2011.01924.x
• 发表时间: 2012-02-01
• 期刊: Functional ecology
• 影响因子: 5.2
• 作者: Place SP;Menge BA;Hofmann GE
• 通讯作者: Hofmann GE