Microbial Genomics and Analytical Laboratory (MGAL) Core

微生物基因组学和分析实验室 (MGAL) 核心

基本信息

批准号：
10712774
负责人：
JOANNE Y YEW
金额：
$ 27.42万
依托单位：
UNIVERSITY OF HAWAII AT MANOA
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-15 至 2028-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10712774
关键词：
Acceleration Aerobic Area Award Bioinformatics Biology Biomedical Research Centers of Research Excellence Communities Complement Data Destinations Development Disease Doctor of Philosophy Ecosystem Education Educational workshop Ensure Environment Equipment Faculty Fostering Gene Expression Genomic Library Genomics Goals Grant Growth Hawaii Hawaiian Health High Pressure Liquid Chromatography High-Throughput Nucleotide Sequencing Human Infrastructure Internships Laboratories Laboratory Study Libraries Lipids Mass Spectrum Analysis Metabolic Metagenomics Methods Microbe Microbiology Modernization Molecular Biology Native Hawaiian Phase Preparation Privatization Proteomics Publications Qualifying Research Research Methodology Research Personnel Research Support Resolution Resources Sampling Scientist Secure Services Shotguns Site Students Taxus Technical Expertise Techniques Technology Time Training Universities Virus Visit analytical method base biobank career data analysis pipeline experience instrumentation interest laboratory facility liquid chromatography mass spectrometry metabolomics microbial microbial genomics microbiome microbiome research microbiota next generation normal microbiota outreach programs recruit searchable database small molecule success tool training opportunity transcriptomics undergraduate student user-friendly

项目摘要

PROJECT SUMMARY – Microbial Genomics and Analytical Laboratory (MGAL) The Microbial Genomics and Analytical Laboratory (MGAL) Core of the Integrative Center for Environmental Microbiomes and Human Health provides a centralized facility and technical expertise at the University of Hawai‘i at Mānoa (UHM) to support the study of the interface between the microbial environment and human health. The characterization of microbiota community composition by high throughput sequencing and metabolite analysis are standard techniques used in the field of microbiome research. The MGAL Core enables access to these key methods by providing the following: (i) services for the preparation of genomic libraries and quantification of gene expression; (ii) high-performance liquid chromatography and mass spectrometry instrumentation for the analysis of lipids and metabolites; and (iii) facilities for the cultivation, isolation, and long-term storage of microbial strains. To expand Core capabilities and user base while continuing to support current and past COBRE researchers, MGAL services will be expanded to include metagenomic and transcriptomic library preparation, metabolomic analysis, and enhanced microbial biobanking facilities that will include the creation of a community searchable database containing information about endemic Hawaiian strains. The MGAL Core is available to researchers throughout the state of Hawai'i and visiting scientists, thus encouraging greater interaction within the local microbiome research community and seeding collaborative efforts from abroad. The facility will beneficially impact COBRE investigators and affiliates by providing essential infrastructure and expertise to advance research and foster the careers of junior faculty. Moreover, the Core will support the continued growth of UHM as a major center for microbiome studies in the Pacific Region by providing hands-on training in library preparation and metabolite analysis, hosting summer workshops on fundamental microbiome research methods, integrating with undergraduate and graduate laboratory classes, and facilitating outreach efforts to characterize Hawai'i’s microbiome.

项目摘要 – 微生物基因组学和分析实验室 (MGAL) 环境综合中心的微生物基因组学和分析实验室 (MGAL) 核心微生物组和人类健康在大学提供集中设施和技术专业知识夏威夷马诺阿 (UHM) 支持微生物环境与人类之间界面的研究通过高通量测序表征微生物群落组成代谢物分析是微生物组研究领域使用的标准技术。通过提供以下服务来获取这些关键方法：(i) 基因组制备服务 (ii) 高效液相色谱和质谱用于分析脂质和代谢物的光谱仪器；以及 (iii) 培养设施；微生物菌株的分离和长期储存，同时扩大核心能力和用户群。为了继续支持当前和过去的 COBRE 研究人员，MGAL 服务将扩大到包括宏基因组和转录组文库制备、代谢组分析和增强微生物生物样本库设施将包括创建包含信息的社区可搜索数据库 MGAL 核心可供整个夏威夷州的研究人员使用。和访问科学家，从而鼓励当地微生物组研究界加强互动该设施将对 COBRE 的研究人员和人员产生有益的影响。附属机构通过提供必要的基础设施和专业知识来推进研究和培养职业生涯此外，该核心将支持 UHM 作为微生物组主要中心的持续发展。通过提供文库制备和代谢物分析方面的实践培训在太平洋地区进行研究，举办关于基本微生物组研究方法的夏季研讨会，与本科生和研究生实验室课程，并促进外展工作以描述夏威夷的微生物组特征。