An enzyme-based assay for the detection of acetaldehyde-protein adducts

用于检测乙醛-蛋白质加合物的酶测定法

• 批准号: 10760201
• 负责人: Kevin K Desai
• 金额: $ 27.21万
• 依托单位: REVEL PHARMACEUTICALS INC.
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10760201
• 关键词: Acetaldehyde; Active Sites; Address; Advanced Glycosylation End Products; Alcohol abuse; Alcohol consumption; Alcoholic Liver Diseases; Alcoholism; Alcohols; Amino Acids; Applications Grants; Biological; Biological Assay; Biological Markers; Blood Glucose; Bovine Serum Albumin; Cattle; Coupled; DNA; Data; Detection; Development; Diabetes Mellitus; Diagnosis; Digestion; Engineering; Enzymes; Escherichia coli; Ethanol Metabolism; Ethanol toxicity; Evolution; Excision; FDA approved; Generations; Genes; Glucose; Glycosylated hemoglobin A; Goals; Health; Hemoglobin; Horseradish Peroxidase; Hydrogen Peroxide; Knowledge; Libraries; Liver; Lysine; Measures; Methodology; Methods; Modeling; Modification; Mutagenesis; Oxidases; Patients; Peptide Hydrolases; Performance; Pharmacologic Substance; Plasmids; Production; Proteins; Reaction; Request for Applications; Sampling; Schiff Bases; Severities; Side; Sorting; Source; Structure; System; Techniques; Technology; Testing; Variant; addiction; adduct; alcohol exposure; alcohol use disorder; chronic alcohol ingestion; design; detection assay; ethyl lysine; genetic selection; glycation; improved; in vitro Assay; member; novel; prototype; rapid detection; repaired; response; screening; sugar; tool

ABSTRACT Chronic alcohol consumption is a significant issue worldwide which can lead to addiction and damage throughout the body. The first step in alcohol metabolism is its conversion into acetaldehyde, which is a highly reactive and toxic molecule. The formation of acetaldehyde adducts with protein and DNA is a primary mechanism of ethanol toxicity. Acetaldehyde adducts are a direct biomarker for alcohol abuse. We intend to develop a facile and rapid enzyme-based assay for measuring acetaldehyde-protein adducts to assess alcohol use and alcohol-related health consequences.

抽象的 慢性饮酒是世界范围内的一个重大问题，可能导致成瘾和损害 遍布全身。酒精代谢的第一步是转化为乙醛，乙醛是一种高度分解的物质。 反应性和毒性分子。乙醛与蛋白质和DNA的加合物的形成是主要的 乙醇毒性机制。乙醛加合物是酒精滥用的直接生物标志物。我们打算 开发一种简便快速的基于酶的测定法，用于测量乙醛-蛋白质加合物以评估酒精 使用和酒精相关的健康后果。