Molecular, Cellular and Genetic Core Component

分子、细胞和遗传核心成分

• 批准号: 7612852
• 负责人: PING-YEE LAW
• 金额: $ 21.69万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-30 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7612852
• 关键词: Animals; Area; Base Sequence; Breeding; Budgets; Cell Line; Cell model; Cellular biology; Charge; Commercial Sources; Communities; Consult; Cost Savings; Development; Equipment and supply inventories; Facility Construction Funding Category; Funding; Genetic; In Vitro; Individual; Libraries; Minnesota; Modeling; Molecular; Mus; Mutant Strains Mice; Neurons; Oligonucleotide Synthesis Facility; Oligonucleotides; Opioid Receptor; Purpose; RNA Interference; Reagent; Recombinant Proteins; Research; Research Personnel; Resources; Services; Small Interfering RNA; Thinking; Universities; Vendor; Viral Vector; bacterial vector; base; cost; expression vector; in vivo; member; mouse model; mutant mouse model; programs; reagent standardization; success; vector

The Molecular Cellular and Genetic Core (MCGC) is being reviving in the Center for the purpose of providing expertise in the areas of molecular and cell biology and assisting in the construction of various vectors used in the proposed studies within the Center. The MCGC will provide all the scientific components the primary cultures from multant mice needed for their studies. The MCGC also will maintain lines of mutant mice that are used by multiple components and are highly requested by the scientific community. By centralizing all these functions in MCGC will allow not only substantial cost saving but also the standardization of the reagents, vectors, cultures and animals in all the projects within the Center. Therefore, the specific aims of MCGC are: 1) To establish banks of reagents and cell models to be used by Center's investigators. The revived MCGC will resume the duties in maintaining the inventories of vectors, bacteria strains, libraries and cell lines generated by the Center investigators from Administrative Core. MCGC will be responsible for sending out any request for the reagents and cell models, and expand the inventories to include viral and vector constructs that are useful for the in vitro primary culture and in vivo animal studies. 2) To provide expertise in the areas of molecular and cell biology to Center's investigators. The MCGC will consult on the construction of the various mammalian expression vectors, or targeting vectors for the development of genetic altered animals. In addition, MCGC will assist in constructing the siRNA vector and viral vectors for delivery the siRNA ortransgenes to be used by the Center's investigators. 3) To maintain a bank of mutant mouse models for the Center's investigators. In order to eliminate the drain in the individual investigator's resources, MCGC will maintain breeding colonies of mouse models so as to fulfill the requests by the Center's investigators and external investigators. 4) To provide primary cultures from wild type and mutant mice for the studies of opioid receptor action. The use of primary cultures as models is a common theme among all 5 scientific components of this proposed Center. Especially, the use of primary neurons from mutant mouse models is critical for establishing the validating of the cell models' observations. Thus, the MCGC will initiate the program of supplying primary cultures to Center's investigators. This program not only could utilize the primary cultures obtained from mutant mice more efficiently, it could also standardize the quality of the primary among all investigators within the Center.

分子细胞和遗传核心 (MCGC) 正在该中心复兴，旨在提供 分子和细胞生物学领域的专业知识，并协助构建各种使用的载体 在中心内拟议的研究中。 MCGC 将提供主要的所有科学组件 研究所需的突变小鼠培养物。 MCGC 还将维持突变小鼠品系 被多个组件使用，并且受到科学界的强烈要求。通过集中所有 MCGC 中的这些功能不仅可以大幅节省成本，还可以实现 中心内所有项目中的试剂、载体、培养物和动物。因此，具体目标 MCGC 的目标是： 1) 建立供中心研究人员使用的试剂和细胞模型库。 恢复后的MCGC将恢复维护载体、菌株、文库库存的职责 以及中心研究人员从管理核心生成的细胞系。 MCGC将负责 发出对试剂和细胞模型的任何请求，并扩大库存以包括病毒和细胞模型 可用于体外原代培养和体内动物研究的载体构建体。 2) 提供 为中心的研究人员提供分子和细胞生物学领域的专业知识。 MCGC将咨询 构建各种哺乳动物表达载体，或用于开发的靶向载体 基因改变的动物。此外，MCGC将协助构建siRNA载体和病毒载体 递送 siRNA 或转基因供中心研究人员使用。 3）维持突变体库 为中心研究人员提供小鼠模型。为了消除个人的流失 根据研究者的资源，MCGC将维持小鼠模型的繁殖群体以满足要求 由中心调查员和外部调查员进行。 4) 提供野生型的原代培养物 和突变小鼠用于研究阿片受体作用。使用原代文化作为模型是 该拟议中心所有 5 个科学组成部分的共同主题。特别是使用初级 来自突变小鼠模型的神经元对于建立细胞模型观察结果的验证至关重要。 因此，MCGC 将启动向中心研究人员提供原代培养物的计划。这 程序不仅可以更有效地利用从突变小鼠获得的原代培养物，还可以 标准化中心内所有调查人员的主要质量。