INIA: Mouse Resources Core

INIA：鼠标资源核心

• 批准号: 7367214
• 负责人: Daniel Goldowitz
• 金额: $ 23.81万
• 依托单位: UNIVERSITY OF BRITISH COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2012-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7367214
• 关键词: Acute; Alcohol consumption; Alcohols; Anxiety; Behavior; Behavioral; Boutos; Chronic; Communities; Complement; Convulsions; Corticosterone; Cryopreservation; Data; Data Base Management; Databases; Elements; Embryo; Equipment and supply inventories; Exhibits; Generations; Genetic; Genotype; Individual; Informatics; Information Dissemination; Knock-out; Knowledge; Maintenance; Measures; Microsatellite Repeats; Modeling; Molecular; Mus; Mutagenesis; Mutant Strains Mice; Neurosecretory Systems; Operative Surgical Procedures; Phenotype; Population; Production; Quality Control; Recombinants; Research Personnel; Resources; Screening procedure; Self Administration; Services; Single Nucleotide Polymorphism; Stress; Testing; Withdrawal; alcohol research; consomic; dependence relapse; experience; high throughput screening; mouse model; mutant; novel; programs

DESCRIPTION (provided by applicant): This is a new core to INIA stress that arises out of the generation of many new mouse models through INIA support over the last 4+ years. These mouse models will be important resources to INIA investigators to study the interplay between stress, anxiety and alcohol consumption. With these new resources, we feel it is critical to disseminate them to users and evaluate each of these new mouse models with a set of INIA relevant behavioral analyses to better identify their usefulness to the various researchers in INIA stress and to the alcohol research community and beyond. The final part of this Core will provide key elements to a mouse core: distribution, cryopreservation, genotyping, and a curatorial function to keep an inventory of mouse mutants and inbred lines that we have available to INIA users and to accumulate and annotate the behavioral, cellular, and molecular knowledge about each mutant and inbred line. The specific aims of this Core are as follows: Aim 1 - The maintenance and distribution of novel mutant lines of mice produced by the ENU-mutagenesis program. The production and availability of novel mouse mutants that have abnormal alcohol, stress, and/or anxiety phenotypes represent an exciting resource for multi-disciplinary studies by our INIA stress investigators. This part of the Core will make these lines of mice available to INIA and community-wide researchers. Aim 2 - The maintenance and distribution of the expanded BXD recombinant inbred (Rl) and B6.A consomic lines of mice. This INIA has greatly expanded the phenotypic "space" (molecular, cellular, and behavioral) associated with unique mouse reference populations and this aspect of the Core will make these mice readily available to INIA researchers. This includes the 50 newly developed BXD Rl lines that arose, in part, from the previous INIA support, and the importation of 86.A consomic lines. Aim 3 - The Behavioral Phenotyping component of this Core will provide more comprehensive phenotypic information about EtOH and stress related behaviors in unique genetic mouse models that have been identified by high throughput behavioral screening within the INIA stress Consortium as exhibiting "extreme" phenotypes for EtOH and/or stress/anxiety responsiveness. Aim 4 - Affiliated functions that include curation of mouse lines in the Core, cryopreservation, database management, genotyping, and dissemination of information.

描述（由申请人提供）：这是INIA应力的新核心，在过去的4年中，通过INIA支持的许多新鼠标模型产生。这些小鼠模型将是INIA研究人员的重要资源，以研究压力，焦虑和饮酒之间的相互作用。有了这些新资源，我们认为将它们传播给用户并通过一组INIA相关的行为分析来评估这些新的鼠标模型至关重要，以更好地确定它们对INIA压力和酒精研究社区及其他地区的各种研究人员的有用性。该核心的最后部分将为小鼠核心提供关键要素：分布，冷冻保存，基因分型和策展功能，以保持我们可用于INIA使用者使用的小鼠突变体和近交系列的清单，并积累和注释有关每个突变和近育线的行为，细胞和分子知识。该核心的具体目的如下：目标1- ENU-刺激计划产生的小鼠的新型突变线的维护和分布。具有异常酒精，压力和/或焦虑表型的新型小鼠突变体的生产和可用性代表了我们的INIA压力研究者多学科研究的令人兴奋的资源。核心的这一部分将使INIA和社区范围内的研究人员可用这些小鼠。 AIM 2-扩展的BXD重组近交（RL）和B6的维护和分布。这种INIA大大扩展了与独特的小鼠参考种群相关的表型“空间”（分子，细胞和行为），核心的这一方面将使这些小鼠很容易被INIA研究人员使用。这包括50个新开发的BXD RL线，部分原因是以前的INIA支持以及86.a综合线的导入。目标3-该核心的行为表型成分将在独特的遗传小鼠模型中提供有关ETOH和应激相关行为的更全面的表型信息，这些信息已通过INIA应力联盟内的高吞吐量行为筛选所识别，作为ETOH和/或压力/焦虑响应能力的“极端”表型。 AIM 4-关联功能，包括核心线中小鼠线的策划，冷冻保存，数据库管理，基因分型和信息传播。