Harvard PRECISION Human Pain Center

哈佛精密人类疼痛中心

基本信息

批准号：
10707416
负责人：
William Russell Renthal
金额：
$ 257.86万
依托单位：
BRIGHAM AND WOMEN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-19 至 2027-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10707416
关键词：
Affect American Animal Model Atlases Autopsy Biological Assay Brain Cells Chronic Communities Data Data Set Ensure Erythromelalgia Explosion Feedback Future Gene Expression Profile Genes Genetic Genetic Variation Genomics Helping to End Addiction Long-term Human Human Genetics Individual Informed Consent Investigation Knowledge Medicine Methods Mission Molecular Monitor Mus Neuroglia Neuroimmune Neuroma Neurons Nociception Nociceptors Opioid Pain Pain Clinics Pain Free Pain management Patients Peripheral Peripheral Nerves Phantom Limb Pain Phenotype Physics Physiological Physiology Population Population Heterogeneity Positioning Attribute Process Productivity Protocols documentation Publications Quality of life Research Research Personnel Sample Size Sampling Sensory Ganglia Technology Therapeutic Tissue Procurements Tissue Sample Tissues Translations Work cell type central sensitization chronic pain chronic pain patient chronic painful condition clinical translation cost effective data harmonization data sharing design effective therapy experience human tissue improved innovation insight meetings multiple omics novel opioid use disorder pain model pain signal single cell technology skills therapeutic target tissue processing tool transcriptomics transmission process tumor-immune system interactions

项目摘要

PROJECT SUMMARY (Overall) Chronic pain affects >25 million Americans per year, with enormous impacts on both quality of life and productivity. Despite advances in our understanding of nociception in animal models, new and effective treatments for patients with chronic pain have been lacking. The poor translation between mouse and human pain targets has highlighted limitations of animal models of pain. Recent advances in single-cell genomics and physiology directly in human tissue position pain researchers to make important new advances with improved opportunities for clinical translation. The Harvard PRECISION Human Pain Center proposes to leverage state- of-the-art single-cell technologies to characterize human nociceptor subtypes and how their gene expression patterns vary across diverse populations (Project 1) as well as in chronic pain conditions that clearly localize to these cells – chronic phantom limb pain associated with painful neuromas (Project 2). The Projects will generate a wealth of data for the scientific community by closely integrating with 5 Cores tasked with 1) procuring high- quality human pain-related tissues following strict regulatory practices, 2) offering the latest single-cell gene multi-omic technologies, 3) performing advanced single-cell spatial transcriptomic analysis, 4) managing, integrating, and distributing all of the data, and 5) administrating and connecting to Center the other PRECISION Human Pain Network Centers. The data generated by our Center will contribute to this broader PRECISION Human Pain Network and to help identify and prioritizate of novel pain therapeutic targets for future investigation.

项目概要（总体）慢性疼痛每年影响超过 2500 万美国人，对生活质量和健康产生巨大影响。尽管我们对动物模型伤害感受的理解有所进步，但新的和有效的。小鼠和人类之间缺乏针对慢性疼痛患者的治疗方法。疼痛目标凸显了疼痛动物模型的局限性。直接研究人体组织的生理学使疼痛研究人员能够通过改进的方法取得重要的新进展哈佛精密人类疼痛中心建议利用国家- 最先进的单细胞技术来表征人类伤害感受器亚型及其基因表达不同人群（项目 1）以及明显局限于慢性疼痛的情况下，模式各不相同这些细胞——与疼痛性神经瘤相关的慢性幻肢痛（项目 2）。通过与 5 个核心紧密结合，为科学界提供丰富的数据，这些核心的任务是 1) 采购高遵循严格监管实践的优质人类疼痛相关组织，2）提供最新的单细胞基因多组学技术，3) 执行先进的单细胞空间转录组分析，4) 管理，集成和分发所有数据，以及 5) 管理和连接到其他 PRECISION 中心人类疼痛网络中心。我们中心生成的数据将有助于实现更广泛的精确性。人类疼痛网络，帮助识别和优先考虑新的疼痛治疗目标，以供未来研究。