Four-dimensional multi-modality microimaging-microdevice system for high throughput drug screening in vivo

用于高通量体内药物筛选的四维多模态显微成像-微器件系统

基本信息

批准号：
10707307
负责人：
Guigen Liu
金额：
$ 17.28万
依托单位：
BRIGHAM AND WOMEN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-30 至 2026-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10707307
关键词：
3-Dimensional 4D Imaging Adoption Award Biomedical Research Biopsy Calibration Catheters Cell Death Cells Clinical Clinical Oncology Color Custom Dependence Development Development Plans Diffusion Doctor of Philosophy Dose Drug Delivery Systems Drug Evaluation Drug Exposure Engineering Environment Evaluation Event Excision Fiber Optics Fluorescence Four-dimensional Future Generations Goals Healthcare Histologic Hospitals Image Imaging Device Imaging technology Immune Implant In Situ In Vitro Intervention Investigation Label Laboratories Laboratory Research Lasers Learning Length Location MC38 Manuscripts Massachusetts Mechanics Mentors Methods Microscopy Needles Optics Organ Paper Patients Pharmaceutical Preparations Procedures Protocols documentation Publications Publishing Quantitative Evaluations Radiology Specialty Raman Spectrum Analysis Reporting Research Research Activity Research Personnel Resolution Resources Signal Transduction Silicon Site Skin Tissue Speed Subcutaneous Tissue System Systems Development Technology Testing Three-Dimensional Imaging Time Tissues Training Training Activity Translating Tumor Tissue Visualization Woman brain tissue career career development clinical application design drug development drug efficacy drug testing experience experimental study flexibility fluorescence imaging high throughput technology high-throughput drug screening imaging probe imaging system immune cell infiltrate in vivo in vivo evaluation in vivo imaging innovation instructor interest leadership development lens medical schools microdevice minimally invasive multimodality optical fiber optical imaging post-doctoral training programs quantitative imaging response sensor technology skill acquisition skills standard of care success tool translational oncology tumor two-dimensional two-photon

3-Dimensional 4D Imaging Adoption Award Biomedical Research Biopsy Calibration Catheters Cell Death Cells Clinical Clinical Oncology Color Custom Dependence Development Development Plans Diffusion Doctor of Philosophy Dose Drug Delivery Systems Drug Evaluation Drug Exposure Engineering Environment Evaluation Event Excision Fiber Optics Fluorescence Four-dimensional Future Generations Goals Healthcare Histologic Hospitals Image Imaging Device Imaging technology Immune Implant In Situ In Vitro Intervention Investigation Label Laboratories Laboratory Research Lasers Learning Length Location MC38 Manuscripts Massachusetts Mechanics Mentors Methods Microscopy Needles Optics Organ Paper Patients Pharmaceutical Preparations Procedures Protocols documentation Publications Publishing Quantitative Evaluations Radiology Specialty Raman Spectrum Analysis Reporting Research Research Activity Research Personnel Resolution Resources Signal Transduction Silicon Site Skin Tissue Speed Subcutaneous Tissue System Systems Development Technology Testing Three-Dimensional Imaging Time Tissues Training Training Activity Translating Tumor Tissue Visualization Woman brain tissue career career development clinical application design drug development drug efficacy drug testing experience experimental study flexibility fluorescence imaging high throughput technology high-throughput drug screening imaging probe imaging system immune cell infiltrate in vivo in vivo evaluation in vivo imaging innovation instructor interest leadership development lens medical schools microdevice minimally invasive multimodality optical fiber optical imaging post-doctoral training programs quantitative imaging response sensor technology skill acquisition skills standard of care success tool translational oncology tumor two-dimensional two-photon

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项目摘要

Project Summary Guigen Liu, Ph.D., is a mechanical and optical engineer whose overarching career goal is to develop and translate optical fiber based biomedical optical imaging and sensing technologies. The research, entitled “Four- dimensional multi-modality microimaging-microdevice system for high throughput drug screening in vivo”, combines the advanced optical microimaging system with an emerging microdevice, which has the huge potential impact on drug development, individualized health care, and fundamental biomedical research. Candidate: Dr. Liu is an Instructor at the Radiology Department of Brigham and Women’s Hospital, Harvard Medical School. During his previous postdoctoral training, he and colleagues pioneered a silicon-tipped fiber- optic sensing platform featuring high speed and high resolution, which earned the 2015 Alan Berman Research Publication Award from the U.S. Naval Research Laboratory. While Dr. Liu has shown a successful track of record in engineering, his training in biomedical research is limited. Through the career development plans: 1) Gain more experience in two-photon fluorescence and Raman microimaging; 2) Learn to design and implement the microimaging-microdevice system; 3) Establish in vivo drug delivery and tissue response testing skills; and 4) Enhance leadership and career development skills, Dr. Liu will launch his independent career in the new field. Mentors/Environment: Dr. Liu has assembled a strong team of mentors to guide him through the proposed training and research activities. The proposed career development plan includes the rich resources available through Brigham & Women’s Hospital and Harvard Medical School, the Tearney Laboratory at the Wellman Center for Photomedicine, and the Laser Biomedical Research Center at Massachusetts Institute of Technology. Research: The research seeks to build an in situ multi-modality optical histological laboratory for the biomedical microdevice, through four specific research aims: 1) To implement quantitative 4D multi-color two-photon fluorescence microimaging; 2) To test drug efficacy in vivo using the 4D two-photon fluorescence MI-MD system; 3) To develop label-free MI-MD system using Raman microscopy; and 4) To investigate microimaging through long and flexible GRIN probes. Completion of these aims will push the microdevice a big step toward potential clinical adoptions in the future. Summary: Innovation of the proposed research is the integration of 3D microimaging and microdevice for 4D testing of drug efficacy and tissue response in vivo, which will meet the pressing needs of high throughput drug screening. The candidate has identified a group of experts who provide complementary training and mentoring on all the aspects for him to complete the proposed research and develop an independent research career.

项目摘要 Guigen Liu博士是一位机械和光学工程师，其总体职业目标是发展和基于光纤的生物医学光学成像和传感技术。该研究名为“四尺寸多模式的微型成像微型电视系统，用于体内高吞吐药物筛查，将高级光学微成像系统与新兴的微电视相结合，该系统具有巨大对药物开发，个性化医疗保健和基本生物医学研究的潜在影响。候选人：刘博士是哈佛大学杨百翰和妇女医院放射科的讲师医学院。在以前的博士后培训中，他和同事们开创了硅尖头的纤维 - 具有高速和高分辨率的光学传感平台，该平台赢得了2015年艾伦·伯曼（Alan Berman）研究美国海军研究实验室的出版奖。刘博士成功地展示了在工程学的记录中，他在生物医学研究方面的培训是有限的。通过职业发展计划：1）在两光子荧光和拉曼微成像上获得更多经验； 2）学会设计和实施微成像 - 微型电视系统； 3）建立体内药物输送和组织反应测试技能；和 4）提高领导力和职业发展技巧，刘博士将在新领域启动他的独立职业。导师/环境：刘博士召集了一个强大的导师团队，以指导他通过该提案培训和研究活动。拟议的职业发展计划包括可用的丰富资源通过Brigham＆妇女医院和哈佛医学院，Wellman的泪液实验室马萨诸塞州理工学院摄影医学中心和激光生物医学研究中心。研究：该研究旨在为生物医学建立原位多模式的光学组织学实验室微电位通过四个特定的研究目的：1）实施定量的4D多色两光子荧光微成像； 2）使用4D两光子荧光MI-MD系统在体内测试药物效率； 3）使用拉曼显微镜开发无标签的MI-MD系统； 4）调查通过长而灵活的笑容问题。这些目标的完成将使微电位迈向潜在的重要一步将来的临床收养。摘要：拟议研究的创新是4D的3D微型成像和微电位的整合在体内测试药物效率和组织反应，这将满足高通量药物的紧迫需求筛选。候选人已经确定了一群提供完整培训和心理的专家关于他完成拟议研究并发展独立研究职业的所有方面。