Core--Chemistry and Analytical

核心--化学与分析

• 批准号: 7466415
• 负责人: Louise M. Ball
• 金额: $ 34.65万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7466415
• 关键词: Adopted; Analytical Chemistry; Area; Basic Science; Biological Assay; Biological Markers; COSY; Chemicals; Chemistry; Chromatography; Circular Dichroism; Complex; Consultations; Data; Detection; Exposure to; Fluorescence; Funding; Gas Chromatography; Goals; Grant; Health; Heterocyclic Compounds; High Pressure Liquid Chromatography; Housing; Human Resources; Individual; Isomerism; Label; Liquid Chromatography; Mass Spectrum Analysis; Measurement; Methodology; Methods; Molecular Structure; NOESY; Nuclear Magnetic Resonance; Nucleosides; Object Attachment; Organism; Outcome; Oxidative Stress; Parents; Play; Polychlorinated Biphenyls; Preparation; Protons; Refractory; Research; Research Infrastructure; Research Personnel; Research Project Grants; Research Support; Role; Route; Sampling; Services; Spectrometry; Spectrum Analysis; Standard Preparations; Standards of Weights and Measures; Structure; System; Techniques; Work; adduct; analytical method; assay development; cost; environmental chemical; instrumentation; method development; novel; programs; protein metabolite; quantum; response; stable isotope; two-dimensional

The Chemistry and Analytical Core of the UNC-CH Superfund Basic Research Program is a Research Support Core that provides support to the Research Projects in the form of analytical techniques and of synthesis, purification and characterization of chemicals, their metabolites and macromolecular adducts that are not available commercially or that can be cost-effectively prepared in-house in bulk quantities. The Chemistry Core offers particular expertise in preparation of standards and internal standards labelled with stable isotopes (15N, 13C, 2H) that allow development of assays using mass spectrometric techniques for highly specific and highly sensitive chermical identification and quantitation, and that enable identification in complex environmental samples of metabolically-relevant organisms that are refractory to traditional culture methods. The goals of the Chemistry and Analytical Core follow closely the aims and accomplishments of the individual research projects, and thus will evolve in response to research needs. The overall Specific Aims of the Chemistry and Analytical Core encompass the following: 1) Provide advice and consultation on analytical methodology and chemical handling The Core provides advice on analytical method development including appropriate choice of analytes, instrumentation and standards. The Core will interact in this manner with all Projects 1- 6. 2) Develop analytical methodology, including gas chromatography and liquid chromatography, for detection and quantitation of parent compounds, metabolites and macromolecular adducts The GC-MS and LC-MS instrumentation of the Biomarkers Mass Spectrometry Facility provides research in methods development. This aim will support all Projects 1 -6. 3) Provide service in carrying out routine assays for detection and measurement by quantitative spectroscopy and mass spectrometry of PAH, PAH metabolites, PCBs, oxidative damage, and macromolecular adducts - Assays for etheno adducts and oxidative damage have been developed and validated, and can be carried out routinely. 4) Prepare novel and rare chemicals, including isotopically-labelled chemicals. Particular expertise has been developed in preparation of nucleobases and nucleosides, PAH and metabolites, PCS metabolites, and protein adducts. This aim will support all Projects. 5) Analysis and structural identification of unknown metabolites and adducts - The Chemistry Core offers expertise in interpretation of spectral data in support of structural elucidation. The Chemistry and Analytical Core of the UNC-CH Superfund Basic Research Program will play a vital role in providing the infrastructure needed for analyses and assays that are critical for evaluating exposure to hazardous environmental chemicals and for understanding the role of oxidative stress in determining health outcomes.

UNC-CH 超级基金基础研究计划的化学和分析核心是一个研究支持核心，以分析技术以及化学物质及其代谢物和大分子加合物的合成、纯化和表征的形式为研究项目提供支持。可以在市场上购买，也可以经济有效地在内部批量制备。化学核心在制备用稳定同位素（15N、13C、2H）标记的标准品和内标物方面提供了特殊的专业知识，允许使用质谱技术开发分析方法，以进行高度特异性和高度灵敏的化学鉴定和定量，并能够在复杂的环境中进行鉴定。传统培养方法难以处理的代谢相关生物体的环境样本。化学和分析核心的目标密切关注各个研究项目的目标和成就，因此将根据研究需求而发展。化学和分析核心的总体具体目标包括以下内容： 1) 提供有关分析方法和化学品处理的建议和咨询 该核心提供有关分析方法开发的建议，包括分析物、仪器和标准的适当选择。核心将以这种方式与所有项目 1-6 相互作用。 2) 开发分析方法，包括气相色谱法和液相色谱法，用于母体化合物、代谢物和大分子加合物的检测和定量。生物标志物质谱设施提供方法开发研究。该目标将支持所有项目 1 -6。 3) 提供定量光谱检测和测量的常规分析服务 PAH、PAH 代谢物、PCB、氧化损伤和大分子的质谱分析 加合物 - 乙烯加合物和氧化损伤的检测方法已经开发和验证，并且可以常规进行。 4) 制备新型稀有化学品，包括同位素标记化学品。在准备工作中已经开发了特殊的专业知识 核碱基和核苷、PAH 和代谢物、PCS 代谢物和蛋白质加合物。这一目标将支持所有项目。 5) 未知代谢物和加合物的分析和结构鉴定 - 化学核心提供光谱数据解释的专业知识，以支持结构阐明。 UNC-CH超级基金基础研究计划的化学和分析核心将在提供分析所需的基础设施方面发挥至关重要的作用 对于评估危险环境化学物质的暴露以及了解氧化应激在确定健康结果中的作用至关重要。