Development of high-quality reference genomes for Anopheles squamosus and An. cydippis

开发鳞状按蚊和按蚊的高质量参考基因组。

• 批准号: 10725180
• 负责人: Yoosook Lee
• 金额: $ 6.27万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-23 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10725180
• 关键词: Acceleration; Accounting; Address; Adult; Anopheles Genus; Area; Beds; Behavior; Biological Assay; Bite; California; Chromosomes; Collection; Complex; Conserved Sequence; Culicidae; DNA Library; DNA sequencing; Data; Development; Diagnostic; First Generation College Students; Florida; Foundations; Genbank; Genes; Genetic; Genetic Polymorphism; Genome; Genomics; Genotype; Goals; Human; Hybrids; Improve Access; Individual; Insecticide Resistance; Insecticides; Larva; Life; Link; Malaria; Manuals; Mitochondria; Modeling; Morphology; Orthologous Gene; Oxidases; Parasites; Phenotype; Plasmodium falciparum; Population; Predisposition; Procedures; Process; Pupa; Rapid diagnostics; Rapid screening; Research; Residual state; Risk; Sampling; Scientist; Siblings; Southern Africa; Sporozoites; Training; Trust; Universities; Vector Ecology; Work; Zambia; barrier to testing; contig; cytochrome c oxidase; design; diagnostic assay; epidemiology study; evidence base; experience; functional genomics; genetic information; genome resource; improved; infection rate; malaria infection; malaria transmission; model organism; neglect; preference; reference genome; scaffold; sequencing platform; therapeutically effective; trait; transcriptome sequencing; transmission process; vector; vector competence; vector control; vector mosquito

Project Summary Despite the tremendous effort to minimize the impacts of malaria in southern Africa, it has yet to be eliminated from this region. Many understudied mosquito vector species inhabit this area and may be perpetuating malaria transmission in this pre-elimination zone. Anopheles squamosus is one of the most abundantly caught mosquito species in southern Zambia. This species is also frequently infected with Plasmodium falciparum, a causal agent of human malaria, leading to the hypothesis that it is a critical vector of malaria transmission in Zambia. A major barrier of testing this hypothesis and studying An. squamosus is the lack of genetic information. In addition to this barrier, An. squamosus is indistinguishable from An. cydippis in the adult stage, making it difficult to establish links between genotypes to phenotypes important for parasite transmission and vector control, including vector competence, insecticide resistance, and host preference. This study addresses these two key barriers by (1) producing high-quality genomes of An. squamosus and An. cydippis, (2) identifying genetic polymorphisms within An. squamosus, and (3) producing gene annotation of An. squamosus genome. The genome of An. squamosus will facilitate the identification of species-specific markers for a diagnostic assay that can be used to distinguish this species from other mosquitoes. Our project will catalyze the study of An. squamosus and other understudied malaria vectors, as well as their impacts on malaria transmission. The new genomic resources for An. squamosus can be leveraged for studying mosquito vector behavior and devise effective control strategies of understudied malaria vectors in pre-elimination settings.

项目概要 尽管为尽量减少南部非洲疟疾的影响做出了巨大努力，但它尚未被消除 来自这个地区。许多未被充分研究的蚊媒物种栖息在该地区，并且可能会永久存在 疟疾在该预消灭区的传播。鳞状按蚊是捕获量最丰富的按蚊之一 赞比亚南部的蚊子种类。该物种还经常感染恶性疟原虫，这是一种 人类疟疾的病原体，导致人们假设它是疟疾传播的关键媒介 赞比亚。检验这一假设和研究 An 的主要障碍。鳞状肌缺乏遗传 信息。除了这个障碍之外，An.鳞状肌与 An 没有区别。成年阶段的cydippis， 使得很难建立对寄生虫传播很重要的基因型和表型之间的联系 病媒控制，包括病媒能力、杀虫剂抗性和寄主偏好。本研究解决 通过 (1) 生产高质量的按蚊基因组来克服这两个关键障碍。鳞状肌和 An.西迪皮斯, (2) 鉴定 An 内的遗传多态性。鳞状，(3)产生An的基因注释。 鳞状细胞基因组。 An 的基因组。鳞状细胞将有助于物种特异性标记的识别 用于可用于区分该物种与其他蚊子的诊断测定。我们的项目将 促进An的研究。鳞状疟原虫和其他未充分研究的疟疾媒介及其对 疟疾传播。 An 的新基因组资源。鳞状细胞可用于研究蚊子 媒介行为并在预消除中制定有效控制策略 设置。