EIB Microscopy Core Facility

EIB 显微镜核心设施

• 批准号: 10703024
• 负责人: Jan Wisniewski
• 金额: $ 38.38万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10703024
• 关键词: Biological Assay; Cells; Computer software; Contract Services; Core Facility; Custom; Equipment; Event; Image; Image Analysis; Imaging Techniques; Immune system; Immunohistochemistry; Immunology; Laser Scanning Confocal Microscopy; Lasers; Microscope; Microscopy; Mission; Procedures; Reagent; Research Personnel; Resolution; Scanning; Standardization; Work; base; cost; design; digital imaging; image processing; instrument; microscopic imaging; new technology; novel; quantitative imaging

The Facility operating expenses primarily include the cost of annual instrument specific service contracts, covering two laser scanning confocal microscopes and a widefield epifluorescence microscope, and purchase of necessary equipment and software upgrades.As new technology becomes available, novel imaging procedures are developed and continually refined to take advantage of the full capabilities of the instruments. Image processing and analysis are developed to quantify and standardize observations made using the imaging based assays. Purchasing of reagents, culture dishes, etc. are generally the responsibility of the specific user, but are borne by the facility when directly related to Dr. Wisniewski's work to develop microscope based assays. The Facility provides start up consumable reagents that are replenished by the user.

设施的运营费用主要包括年度特定工具服务合同的成本，涵盖了两个激光扫描共聚焦显微镜和广阔的落水显微镜，以及购买必要的设备和软件升级的购买。随着新技术的可用，开发了新的成像程序，并不断地进行了精制以利用仪器的全部功能。开发图像处理和分析是为了量化和标准化使用基于成像的测定法进行的观测值。 购买试剂，培养皿等通常是特定用户的责任，但与Wisniewski博士开发基于显微镜的测定法的工作直接相关时是由该设施承担的。该设施提供了用户补充的启动消耗试剂。