Development of Sensitive and Specific Proteomic Biomarkers of Aging, Health, Frailty, and Morbidity in Human Cohorts

开发人类群体中衰老、健康、虚弱和发病的敏感和特异性蛋白质组生物标志物

• 批准号: 10688780
• 负责人: Nathan Basisty
• 金额: $ 5.77万
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10688780
• 关键词: Age; Aging; Baltimore; Biological Assay; Biological Markers; Biomedical Research; Biopsy; Blood; Clinical; Cohort Studies; Data; Development; Disease; Elderly; Geographic Locations; Goals; Health; Human; Individual; Italy; Literature; Longitudinal Studies; Mass Spectrum Analysis; Morbidity - disease rate; Muscular Atrophy; Nerve Degeneration; Non-Insulin-Dependent Diabetes Mellitus; Pathology; Plasma; Population Study; Proteins; Proteomics; Research Project Grants; Running; Sampling; Sensitivity and Specificity; Specimen; Techniques; Technology; Testing; Therapeutic; Therapeutic Intervention; Tissues; Translations; United States; Validation; Variant; Work; age related; base; biomarker panel; biomarker signature; burden of illness; candidate marker; cell type; clinical biomarkers; cohort; frailty; gene product; human old age (65+); human tissue; improved; monocyte; next generation; novel; predictive marker; protein biomarkers; senescence

Building on our previous efforts to develop senescence biomarker signatures, we have focused our efforts on the discovery of a novel set of senescence biomarkers found in monocytes, a cell type that is both proximal to the blood and known to increase in senescence with age in humans. In the past year, we have established a senescent monocyte culture and performed large-scale proteomic analysis of senescent human monocytes. Further, to test this cell-type specific set of biomarkers and its relationship to aging and diseases in humans, we are initiating studies in several cohorts: GESTALT, BLSA, and InCHIANTI, to correlate a new set of biomarker candidates with human health, frailty, and other clinical parameters. The purpose and scope of this research project going forward is to develop and apply state-of-the-art mass spectrometry based proteomic techniques to discover and validate subsets of proteins and protein forms that are representative of aging and disease burdens in human tissues, blood, and other biofluids.

在我们先前开发衰老生物标志物特征的努力的基础上，我们将精力集中在发现单核细胞中发现的一套新型衰老生物标志物上，这种细胞类型既靠近血液，又已知会随着人类年龄的增长而闻名。在过去的一年中，我们建立了衰老的单核细胞培养物，并对人类单核细胞进行了大规模的蛋白质组学分析。此外，为了测试这种细胞类型的特定生物标志物及其与人类衰老和疾病的关系，我们正在启动几个同类的研究：Gestalt，blsa和Inchianti，将一组新的生物标志物候选者与人类健康，脆弱的候选者和其他临床参数相关联。 该研究项目的目的和范围是开发和应用基于质谱的最先进的蛋白质组学技术，以发现和验证代表人类组织，血液和其他生物流体中衰老和疾病负担负担的蛋白质和蛋白质形式的亚群。