CORE 1 - Animal Phenotyping Core

核心 1 - 动物表型核心

• 批准号: 10657781
• 负责人: PHILIPP E SCHERER
• 金额: $ 16.4万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10657781
• 关键词: Animal Model; Animals; Biological Assay; Blood; Blood Chemical Analysis; Body Composition; Calorimetry; Cells; Cholesterol; Collection; Communication; Data; Disease; Dual-Energy X-Ray Absorptiometry; Enzyme-Linked Immunosorbent Assay; Equipment; Food; Genus Hippocampus; Glucose; Glucose Clamp; Glycolysis; Goals; Guidelines; Hepatocyte; Hormones; Hyperinsulinism; Individual; Laboratories; Lipids; Magnetic Resonance Imaging; Maps; Measurement; Measures; Metabolic; Metabolism; Mitochondria; Mus; Obesity; Pathogenesis; Phenotype; Physiological; Quality Control; Rattus; Reagent; Research; Research Personnel; Respiration; Services; Speed; Standardization; Technical Expertise; Technology; Testing; Tissues; Triglycerides; Urine; Water consumption; Work; cost effective; cytokine; extracellular; glucose and insulin clamps; glucose tolerance; instrumentation; insulin tolerance; magnetic beads; microCT; nutrition; pre-clinical; prevent; sound; temporal measurement; treadmill

PROJECT SUMMARY/ABSTRACT – Core 1 – Animal Phenotyping/Metabolism Core The services provided in the Animal Phenotyping/Metabolism Core draw upon the expertise of many investigators at UTSW to provide comprehensive analytical capabilities that are on par with the best facilities in the world. We established our Animal Phenotyping/Metabolism Core more than 12 years ago and we have continued to expand our assay capabilities to support investigators with in-depth preclinical capabilities. The availability of standardized assays speeds the pace of our research, facilitates inter-lab comparisons of data, and provides a common platform for the necessary close interactions between investigators of the NORC. Animal models that allow careful characterization of the metabolic effects of obesity and that have an altered communication axis between critical tissues and are vital to understand the pathogenesis of disease. Given the number of phenotypic measurements, the range of target metabolites, and the number of different animal models investigated among the NORC investigators, it is necessary to provide uniform measurements and to have quality control guidelines in place. To facilitate the research of NORC investigators, Core 1 will provide comprehensive, state-of-the-art measurements of multiple metabolic parameters, with measurements involving physiological read outs that include lipid, glucose, and metabokine assays. The technical expertise offered by the Animal Phenotyping/Metabolism Core will bring multiple benefits to NORC investigators, including but not limited to the following:  Centralized Core services that are more cost effective and prevent the duplication of reagents across NORC investigators.  Provides consistency in various assays across laboratories.  Provides consistent quality control measures.  Makes available technically challenging assays, such as hyperinsulinemic-euglycemic clamps, that cannot be established in individual laboratories.  Makes available equipment that is essential for nutrition and obesity research but that is too expensive for individual investigators to purchase (e.g. Seahorse, MRI, metabolic cages, animal CT).  Frees investigators from routine work to focus on the intellectual challenges of the projects.

项目摘要/摘要 - 核心1 - 动物表型/代谢核心 动物表型/代谢核心中提供的服务取决于许多人的专业知识 UTSW的调查人员提供与最佳设施相当的全面分析功能 世界。我们在12年前建立了动物表型/代谢核心，我们已经 继续扩展我们的评估能力，以支持具有深入的临床前能力的研究人员。 标准化测定的可用性加快了我们研究的空间，促进了数据间比较数据的比较， 并为NORC研究人员之间的必要紧密互动提供了一个共同的平台。 动物模型可以仔细表征肥胖的代谢作用并改变的动物模型 关键组织之间的通信轴对了解疾病的发病机理至关重要。鉴于 表型测量的数量，靶代谢物的范围和不同动物模型的数量 在NORC调查人员之间进行了调查，有必要提供统一的测量并具有 制定了质量控制指南。为了促进NORC调查人员的研究，Core 1将提供 多个代谢参数的全面，最先进的测量，并进行测量 涉及物理读物，包括脂质，葡萄糖和元代表分析。 动物表型/代谢核心提供的技术专长将为NORC带来多重好处 调查人员，包括但不限于以下内容： 集中的核心服务，这些核心服务更具成本效益，并防止试剂重复 NORC调查人员。 在实验室之间提供各种测定法。 提供一致的质量控制措施。 在技术上挑战测定法，例如高胰岛素血糖夹具，即 不能在个别实验室中建立。 提供对营养和肥胖研究至关重要的设备，但这太贵了 供各个研究人员购买（例如，海马，MRI，代谢笼，动物CT）。 将调查人员摆脱常规工作，重点关注项目的智力挑战。