Rare earth nanoprobes for optical imaging and disease tracking (C3i)

用于光学成像和疾病追踪的稀土纳米探针 (C3i)

基本信息

批准号：
10663617
负责人：
PRABHAS V MOGHE
金额：
$ 15.48万
依托单位：
RUTGERS, THE STATE UNIV OF N.J.
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-04-15 至 2023-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10663617
关键词：
AMD3100 Administrative Supplement Albumins Animal Model Animals Behavior Biological Assay Biological Markers Body Weight CXCR4 gene Cancer Model Clinical Trials Contrast Media Data Disease Disseminated Malignant Neoplasm Drug Kinetics Elements Encapsulated Erbium Ethanol Exhibits Fluorescence-Activated Cell Sorting Future Glutaral Human Image In Vitro Individual Knowledge Ligands Light Liquid substance Measurement Metabolic Methods Microfluidic Microchips Microfluidics Mus National Institute of Biomedical Imaging and Bioengineering Output Parents Performance Phase Planet Earth Polymers Process Production Property Proteins Reaction Research Serum Albumin Short Waves Signal Transduction Stream Surface Techniques Technology Time Tumor Markers biomaterial compatibility chemokine receptor commercialization contrast imaging crosslink experimental study fluorescence-guided surgery in vivo instrumentation light scattering mouse model nanoparticle nanoprobe nephelometry optical emission optical imaging overexpression parent grant programs prototype residence scale up small molecule targeted biomarker

项目摘要

PROJECT SUMMARY / ABSTRACT This project is a C3i Accell administrative supplement to our ongoing R01 project (EB018358 “Rare-earth nanoprobes for optical imaging and disease tracking”). The parent R01 project is developing short-wave infrared (SWIR) light-emitting nanoparticle contrast agents and imaging instrumentation for use in fluorescence-guided surgery. In the R01 project, mouse models of cancer are employed to evaluate the performance of these contrast agents in vivo. Through the core C3i program, we learned that successful commercialization of this technology will require us to demonstrate that contrast agent synthesis can be scaled to much larger volumes than used in our R01 murine studies, while maintaining quality and consistency. We currently synthesize nanoprobes in individual 15 ml batches which are sufficient to complete a typical longitudinal murine experiment, but inadequate for moving the technology forward into large animal models and in-human clinical trials. To enable these future studies, this C3i Accell project will develop and validate methods to synthesize nanoprobes at the 1000 ml scale. Nanoprobes will contain an erbium-doped nanoparticle core providing SWIR optical emissions, encapsulated in human serum albumin for biocompatibility, with the small molecule AMD3100 conjugated to the outer surface for targeting the CXCR4 tumor biomarker. To maintain uniformity in nanoprobe properties at this scale and to demonstrate a scalable approach for commercial production, we will develop synthesis methods using a continuous flow microfluidic platform.

项目概要/摘要该项目是对我们正在进行的 R01 项目（EB018358“稀土纳米探针光学成像和疾病追踪”）母项目 R01 正在开发短波红外（SWIR）发光。用于荧光引导手术的纳米颗粒造影剂和成像仪器在 R01 项目中。采用癌症小鼠模型来评估这些造影剂的体内性能。通过核心 C3i 计划，我们了解到这项技术的成功商业化需要我们证明造影剂合成的体积可以比我们的 R01 小鼠研究中使用的体积大得多，同时保持质量和一致性，我们目前以单独的 15 毫升批次合成纳米探针。足以完成典型的纵向小鼠实验，但不足以将该技术推进到为了实现这些未来的研究，这个 C3i Accell 项目将开发和进行。验证 1000 毫升规模的纳米探针的合成方法纳米探针将包含掺铒纳米粒子。核心提供短波红外光发射，封装在人血清白蛋白中以实现生物相容性，具有小 AMD3100 分子缀合到外表面，用于靶向 CXCR4 肿瘤生物标志物，以保持一致性。纳米探针在这种规模上的特性，并展示商业生产的可扩展方法，我们将开发使用连续流微流体平台的合成方法。

项目成果

期刊论文数量（15）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Surface-Modified Shortwave-Infrared-Emitting Nanophotonic Reporters for Gene-Therapy Applications.

DOI：
10.1021/acsbiomaterials.8b00378
发表时间：
2018-07-09
期刊：
ACS biomaterials science & engineering
影响因子：
5.8
作者：
Zhao Z;Kantamneni H;He S;Pelka S;Venkataraman AS;Kwon M;Libutti SK;Pierce M;Moghe PV;Ganapathy V;Tan MC
通讯作者：
Tan MC

Bioadsorption of Rare Earth Elements through Cell Surface Display of Lanthanide Binding Tags.

通过细胞表面显示镧系元素结合标签来生物吸附稀土元素。

DOI：
10.1021/acs.est.5b06129
发表时间：
2016-03-01
期刊：
Environmental science & technology
影响因子：
11.4
作者：
Park DM;Reed DW;Yung MC;Eslamimanesh A;Lencka MM;Anderko A;Fujita Y;Riman RE;Navrotsky A;Jiao Y
通讯作者：
Jiao Y

Biodegradable rare earth fluorochloride nanocrystals for phototheranostics.

用于光治疗的可生物降解稀土氟氯化物纳米晶体。

DOI：
10.1039/d0ra00760a
发表时间：
2020
期刊：
RSC advances
影响因子：
3.9
作者：
Zhao,Xinyu;Yu,Qi;Yuan,Jun;Thakor,NitishV;Tan,MeiChee
通讯作者：
Tan,MeiChee

CXCR-4 Targeted, Short Wave Infrared (SWIR) Emitting Nanoprobes for Enhanced Deep Tissue Imaging and Micrometastatic Cancer Lesion Detection.

DOI：
10.1002/smll.201502202
发表时间：
2015-12-16
期刊：
Small (Weinheim an der Bergstrasse, Germany)
影响因子：
0
作者：
Zevon M;Ganapathy V;Kantamneni H;Mingozzi M;Kim P;Adler D;Sheng Y;Tan MC;Pierce M;Riman RE;Roth CM;Moghe PV
通讯作者：
Moghe PV

Shortwave Infrared-Emitting Theranostics for Breast Cancer Therapy Response Monitoring.

DOI：
10.3389/fmolb.2020.569415
发表时间：
2020
期刊：
Frontiers in molecular biosciences
影响因子：
5
作者：
Shah JV;Gonda A;Pemmaraju R;Subash A;Bobadilla Mendez C;Berger M;Zhao X;He S;Riman RE;Tan MC;Pierce MC;Moghe PV;Ganapathy V
通讯作者：
Ganapathy V