Fetal exposure to environmental toxins & infant outcome

胎儿接触环境毒素

• 批准号: 7028942
• 负责人: Enrique Mapua Ostrea
• 金额: $ 53.98万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7028942
• 关键词: atomic absorption spectrometry; behavior test; biomarker; blood chemistry; child physical development; child psychology; clinical chemistry; clinical research; cord blood; embryo /fetus toxicology; environmental exposure; environmental toxicology; feces; feces analysis; gas chromatography mass spectrometry; hair; human pregnant subject; infant human (0-1 year); metal poisoning; newborn human (0-6 weeks); pesticide biological effect; pesticide residues; preschool child (1-5)

DESCRIPTION (provided by applicant): The exposure of pregnant women to environmental toxins is of major concern because of their potential harm on the fetus. However, the detection of fetal exposure to environmental toxins still remains a major challenge. We propose that meconium analysis is a promising tool to meet this challenge. Aims: (1) To compare the prevalence and amount of fetal exposure to environmental toxins through the analysis of meconium, cord blood and neonatal hair and to determine the degree of agreement among these three methods, (2) to determine the relationship between the prevalence and amount of maternal exposure to environmental toxins during pregnancy, as determined by serial analyses of maternal hair and blood, to the prevalence and amount of fetal exposure to environmental toxins as determined by meconium, cord blood and neonatal hair analyses, and (3) to compare adverse immediate (birth weight, length, head circumference, gestational age) and long term (postnatal growth and neurobehavioral development up to 2 yrs from enrollment) outcomes that are associated with antenatal exposure to environmental toxins as determined by maternal blood, maternal hair, meconium, cord blood and neonatal hair analyses. Study design: Pregnant women (n=750) will be recruited, at midgestation, from the Outpatient Clinic of the Bulacan Provincial Hospital, Philippines and their blood and hair will be obtained at the time of recruitment and at delivery. Umbilical cord blood, meconium and neonatal hair will also be obtained. The samples will be analyzed, by atomic absorption spectrometry, for lead, mercury and cadmium and by gas chromatography/mass spectrometry for the following pesticides and their metabolites: propoxur, transfluthrin, Malathion, DDT, chlorpyrifos, bioallethrin, pretilachlor, lindane, cyfluthrin and cypermethrin. Pertinent maternal and infant data will be obtained after birth. The infants will be subsequently followed up at scheduled intervals for 2 years, to study their physical growth and neurobehavioral development using a battery of tests. Data analysis: The relationship between the presence/amount of environmental toxins in meconium, maternal blood, maternal hair, cord blood or neonatal hair to the immediate and two year outcome in the infants will be studied, while controlling for potential confounders. The presence/amount of environmental toxins in maternal blood, hair, cord blood, meconium and neonatal hair will be also evaluated to determine which substrate (s) provide(s) the best index of exposure for a given toxin. Expected benefits: Meconium analysis may provide a powerful tool to study the prevalence and degree of fetal exposure to environmental toxins and its associated adverse effects. This project can also serve as a model for the study of environmental pollutant problems during pregnancy at a local, national or global level.

描述（由申请人提供）：孕妇暴露于 环境毒素因其对人类的潜在危害而备受关注 胎儿。然而，胎儿接触环境毒素的检测仍然存在 仍然是一个重大挑战。我们认为胎便分析是一种很有前景的方法 来应对这一挑战的工具。目的： (1) 比较患病率和数量 通过胎便、脐带分析，胎儿暴露于环境毒素 血液和新生儿头发，并确定这些之间的一致程度 三种方法，（2）确定患病率与 母亲在怀孕期间接触环境毒素的量， 通过对母亲头发和血液的系列分析确定患病率和 通过胎便确定胎儿接触环境毒素的量， 脐带血和新生儿毛发分析，以及（3）比较不良的即时结果 （出生体重、身长、头围、孕龄）和长期 （入组后 2 年内的产后生长和神经行为发育） 与产前接触环境毒素有关的结果 由母血、母体毛发、胎便、脐带血和新生儿决定 头发分析。研究设计：将招募孕妇（n = 750）， 妊娠中期，来自布拉干省立医院门诊部， 菲律宾人和他们的血液和头发将在 招募和交付时。脐带血、胎便和新生儿毛发 也将获得。将通过原子吸收分析样品 铅、汞和镉的光谱测定以及气相色谱/质谱法 以下农药及其代谢物的光谱测定：残杀威、 四氟苯菊酯、马拉硫磷、滴滴涕、毒死蜱、生物丙烯菊酯、丙草胺、 林丹、氟氯氰菊酯和氯氰菊酯。相关母婴数据将 出生后即可获得。婴儿随后将在 计划间隔两年，研究他们的身体发育和 使用一系列测试来进行神经行为发育。数据分析： 胎粪中环境毒素的存在/数量之间的关系， 母体血液、母体头发、脐带血或新生儿头发直接和 将研究婴儿两年的结果，同时控制 潜在的混杂因素。母体内环境毒素的存在/数量 血液、头发、脐带血、胎便和新生儿毛发也将被评估 确定哪种基材能够提供给定条件下的最佳暴露指数 毒素。预期效益：胎便分析可能提供强大的工具 研究胎儿接触环境毒素的普遍性和程度 其相关的不利影响。这个项目也可以作为一个模型 地方、国家妊娠期环境污染物问题研究 或全球水平。