Divergent Functional and Metabolic Development of the Infant Microbiome

婴儿微生物组的不同功能和代谢发育

基本信息

批准号：
10652430
负责人：
Susan Veronica Lynch
金额：
$ 31.04万
依托单位：
UNIVERSITY OF ARIZONA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-07-10 至 2026-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10652430
关键词：
2 year old Allergic Arizona Asthma Bacteria Biological Assay Birth Border Community Border Crossings Cells Childhood Childhood Asthma Data Development Disparate Disparity Electrical Resistance Environment Environmental Exposure Epithelium Exhibits FOXP3 gene Feces Frequencies Genes Genetic House Dust Human IL4 gene IgE Immune Immunity In Vitro Infant Infant Development Inherited Intestines Life Location Meconium Medicine Metabolic Metabolic dysfunction Metagenomics Mexican Mexico Microbe Mothers Nature Neonatal Newborn Infant Outcome Pattern Population Preparation Prevalence Production Relative Risks Residencies Resolution Ribosomal RNA Risk Sampling Shotguns Source T-Lymphocyte Testing Vagina Water Supply Wheezing Woman atopy cohort drinking water experience fetal gut inflammation gut microbiome gut microbiota high risk high risk infant immune function immunological status immunoregulation in utero infancy infant gut microbiome maternal microbiome member metabolomics microbial microbial composition microbiome microbiome alteration microbiome components microbiome composition microbiome research microbiota mouse model neonate offspring peer pregnant prenatal stool sample transcriptome sequencing transmission process vaginal microbiota water sampling

项目摘要

BEAMS ABSTRACT: Project 2 Diminished early life environmental microbial exposures and perturbations to the infant gut microbiome are related to asthma outcomes in childhood. Mexican populations resident in Nogales, Mexico exhibit a four-fold decrease in childhood asthma rates compared with a population of comparable ancestry resident seventy miles away across the border in Tucson, Arizona. Our preliminary data has demonstrated that these cross-border populations experience significantly distinct microbial exposures in their environment (house dust and drinking water) and that the stool microbiota of 1-month old infants raised in these environments are differentially enriched for immunomodulatory bacterial genera. This suggests that microbial differentials in environmental and personal microbiomes relate to the cross-border disparity in asthma rates observed in this population. BEAMS proposes to comprehensively examine a population of Mexican mother-infant dyads resident in Nogales, Mexico or Tucson, Arizona and their environment in early life to determine how differences in environmental, maternal and infant microbial composition and function relate to asthma disparities in this cross-border population. Project 2 will examine at high-resolution the functional attributes of the microbiome of a subset of paired maternal prenatal (vaginal and stool) and infant gut (longitudinally collected stool) samples collected from 200 mother-infant dyads, as well as their paired house dust and water samples using shotgun metagenomics, RNA-Seq and metabolomics. The study will examine functional differentials in inherited microbes and gut microbiome development in cross-border communities that relate to T2 wheezing at age 2 years (a robust predictor of asthma in later childhood) and immune development. These efforts aim to identify environmental, maternal and infant microbiome attributes that promote protection against asthma within Mexican populations.

梁摘要：项目2 早期生活环境微生物暴露和对婴儿肠道微生物组的扰动的减少是与童年时期的哮喘结果有关。居住在墨西哥Nogales的墨西哥人口展示了四倍与可比血统居民七十英里相比，童年哮喘率的降低穿越亚利桑那州图森的边界。我们的初步数据表明这些跨境人群在环境中经历了显着不同的微生物暴露（房屋灰尘和饮酒水）和在这些环境中饲养的1个月大婴儿的粪便微生物群体有差异用于免疫调节细菌属。这表明环境和个人的微生物差异微生物组与该人群中观察到的哮喘率的跨境差异有关。梁提出全面检查墨西哥Nogales居住的墨西哥母亲二元组的人口图森，亚利桑那州及其在早期的环境，以确定环境，母性和孕产妇的差异婴儿微生物组成和功能与这种跨境种群中的哮喘差异有关。项目2 将在高分辨率上检查成对母体产前子集的微生物组的功能属性（阴道和凳子）和婴儿肠道（纵向收集的凳子）样品，从200个母亲二元组中收集以及使用shot弹枪元素学，RNA-Seq和代谢组学。该研究将检查遗传微生物和肠道微生物组中的功能差异跨境社区的发展与2岁时T2喘息有关（哮喘的强大预测指标在童年后期）和免疫发育。这些努力旨在确定环境，母亲和婴儿微生物组属性可促进墨西哥人群中哮喘的保护。