Tissue Procurement and Processing Core

组织采购和加工核心

• 批准号: 10646151
• 负责人: Maki Nakayama
• 金额: $ 17.74万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10646151
• 关键词: Adipocytes; Agreement; Alberta province; Antibodies; Antigens; Basic Science; Blood specimen; Cell Line; Cells; Clinical; Collaborations; Colorado; Communities; Complement; Consultations; Diabetes Mellitus; Disease model; Drug or chemical Tissue Distribution; Equipment; Formalin; Freezing; Funding; Generations; Histologic; Histology; Histopathology; Human; Immune; Individual; Institutional Review Boards; International; Letters; Maintenance; Mission; Non-Insulin-Dependent Diabetes Mellitus; Operative Surgical Procedures; Organoids; Pancreas; Paraffin Embedding; Paraffin Tissue; Pathogenesis; Pathology; Patients; Preparation; Procedures; Protocols documentation; Reagent; Research; Research Personnel; Resources; Rodent; Sampling; Services; Source; Spleen; Stains; Surveys; Thymus Gland; Tissue Embedding; Tissue Procurements; Tissue Sample; Tissues; Training; Universities; Work; base; biobank; cell bank; cell repository; human pluripotent stem cell; human tissue; innovation; instrument; islet; liquid crystal polymer; material transfer agreement; member; tissue preparation; tissue processing

TISSUE PROCUREMENT AND PROCESSING CORE PROJECT SUMMARY/ABSTRACT The overall mission of the Tissue Procurement and Processing (TPP) Core is to facilitate access and usage of tissues and services to meet the specialized needs of the diabetes research community to expedite and catalyze innovative diabetes research at UC AMC. This core will establish and expand resources to assist DRC investigators with the acquisition and processing of rodent and human tissue, and specialized cell lines that are broadly required for diabetes research. For the past 20 years, the services provided by the BDC-based facility has provided high quality tissues to BDC investigators. DRC funding would allow us to expand access to all DRC investigators studying the pathogenesis of Type1 (T1D) and Type 2 (T2D) diabetes. In addition, there is increased need for primary human tissue. We have established a centralized facility to facilitate access to human tissues from external sources (University of Alberta, Edmonton; see letter of support), and internally through the UC-AMC Pathology Biorepository/Tissue Distribution Facility (see letter of support). Furthermore, administrative support for the preparation of IRB approval, human exempt protocols and material transfer agreements for these tissues will be available upon request by DRC members. The core will also provide diabetes-related histopathology services, training in basic and specialized sample preparation and processing procedures, and will offer DRC members access to resources such as validated commonly used reagents and equipment. Finally, the TPP Core will provide a cell banking service to distribute validated diabetes-related cell lines to DRC investigators. These services will facilitate individual studies, in addition to promoting collaborations between investigators. Major services to be provided by the TPP core include 1) tissue preparation, including rodent and human islet isolation and processing; 2) procurement of primary human tissues from local, national and international sources; 3) sample processing for histological analysis; 4) validated and optimized reagents, such as antibodies and probes; and 5) validated cell line distribution. These services have been selected based on a campus-wide survey that solicited information from UC AMC diabetes investigators about their research needs. These services have been selected based on a campus-wide survey that solicited information from UC AMC diabetes investigators about their research needs. More than 65% of our DRC membership, which represents >95% of our non-clinical research base indicated their need for tissue procurement and/or processing assistance.

组织采购和加工核心 项目摘要/摘要 组织采购和加工（TPP）核心的总体任务是促进和使用 组织和服务满足糖尿病研究社区的专业需求，以加快和催化 UC AMC的创新糖尿病研究。该核心将建立和扩展资源以协助刚果民主共和国 研究人员通过啮齿动物和人体组织的获取和处理，以及专门的细胞系 糖尿病研究大致必需。在过去的20年中，基于BDC的设施提供的服务 为BDC研究人员提供了高质量的组织。刚果民主共和国的资金将使我们能够扩大对所有刚果民主共和国的访问权限 研究类型1（T1D）和2型（T2D）糖尿病的发病机理的研究者。此外，还有 对原代人组织的需求增加。我们已经建立了一个集中式设施，以促进进入人类的机会 来自外部来源的组织（艾伯塔大学，埃德蒙顿大学；请参见支持信），并在内部通过 UC-AMC病理学生物验证/组织分布设施（请参阅支持信）。此外，行政管理 支持准备IRB批准，人类豁免方案和物料转让协议 drc成员可以要求提供组织。核心还将提供与糖尿病有关的 组织病理学服务，基本和专业样本准备和处理程序的培训以及 将为刚果民主共和国成员访问诸如经过验证的常用试剂​​和设备之类的资源。最后， TPP核心将提供单元银行服务，将与糖尿病有关的细胞系分发给DRC 调查人员。这些服务将促进个人研究，除了促进 调查人员。 TPP核心提供的主要服务包括1）组织准备，包括啮齿动物和 人类胰岛隔离和处理； 2）从地方，国家和 国际资料来源； 3）样品处理进行组织学分析； 4）经过验证和优化的试剂， 作为抗体和探针； 5）验证的单元线分布。这些服务是根据 校园范围内的调查向UC AMC糖尿病研究人员征求有关其研究需求的信息。 这些服务是根据在校园范围内的一项调查中选择的，该调查从UC AMC征集了信息 糖尿病研究人员的研究需求。我们的刚果民主共和国会员资格的65％以上 我们的非临床研究基础> 95％表明他们需要组织采购和/或加工援助。