Regulation of RIG-I signaling and viral immune evasion by ufmylation

通过 ufmylation 调节 RIG-I 信号传导和病毒免疫逃避

基本信息

批准号：
10414114
负责人：
Stacy Michelle Horner
金额：
$ 45.67万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-06-01 至 2026-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10414114
关键词：
Antiviral Agents Antiviral Response Antiviral Therapy Autoimmune Diseases Biological Response Modifiers Cell physiology Cells Complex Data Dengue Dengue Infection Dengue Virus Development Disease Flavivirus Genes Goals Human Cell Line IRF3 gene Immune Immune Evasion Immune system Immunotherapeutic agent Infection Innate Immune Response Interferons Knowledge Lead Ligase Lysine Mediating Membrane Molecular Molecular Chaperones Morbidity - disease rate Natural Immunity Pathway interactions Pattern recognition receptor Phosphorylation Phosphotransferases Play Post-Translational Protein Processing Process Proteins Public Health RNA Virus Infections RNA Viruses Receptor Signaling Regulation Role Signal Pathway Signal Transduction Signaling Protein System TBK1 gene Testing Therapeutic Time Ubiquitin Ubiquitination Viral Viral Proteins Virus Virus Diseases Virus Replication Work ZIKA antiviral immunity base design emerging pathogen improved innate immune pathways innovation monocyte mortality novel prevent programs protein function protein phosphatase inhibitor-2 response ubiquitin-protein ligase vaccine strategy

项目摘要

ABSTRACT Type I and III interferons (IFN) restrict RNA virus infection. Infected cells produce IFN through signaling activated by pattern recognition receptors such as RIG-I. Both RIG-I and downstream signaling must be highly coordinated for efficient antiviral responses. As such, these processes are regulated by several post-translational modifications (PTMs). These PTMs are essential for both the activation and eventual termination of RIG-I- signaling. While RIG-I-signaling is coordinated by ubiquitination and phosphorylation, the mechanisms by which other PTMs, such as ubiquitin-like modifiers, may regulate RIG-I-signaling are largely unknown. Our preliminary data reveal that the ubiquitin-like modifier called ufmylation regulates multiple proteins involved in RIG-I signaling for optimal IFN induction in response to viral infection. Further, our data suggest that ufmylation is utilized by viruses to evade the host intracellular innate immune response. Therefore, the goal of this proposal is to determine how ufmylation regulates the intracellular innate immune response to virus infection and viral evasion. Based on our preliminary data, the central hypothesis is that ufmylation modulates the function of host and viral proteins to regulate antiviral innate immunity and viral evasion. Guided by our preliminary data, this hypothesis will be tested by pursuing the following three specific aims: 1) Define how UFL1, the ufmylation E3 ligase, promotes the activation of RIG-I; 2) Understand the molecular mechanism by which ufmylation of a key protein in the RIG-I signaling pathway downregulates its function and signaling; 3) Determine how dengue virus co-opts the ufmylation conjugation system for immune evasion. In Aim 1, the molecular mechanisms by which UFL1 induces the activation of RIG-I in response to RNA virus sensing will be defined. In Aim 2, the mechanism and dynamics of how ufmylation regulates the function of a signaling protein in RIG-I pathway will be determined. In Aim 3, the function by which ufmylation of a DENV protein promotes DENV immune evasion, both in human cell lines and in primary cells, will be determined. Taken together, the work proposed in this application will be significant and innovative because it will attribute a novel immune regulatory function to ufmylation, contribute to our understanding of its basic functions, and uncover a novel control (ufmylation) of antiviral innate immunity. Additionally, this work will provide understanding of a host-directed process that is utilized by viruses for immune evasion to facilitate viral replication. Overall, this work will define a new PTM that coordinates the RIG-I signaling pathway, which will improve our knowledge of both antiviral immunity and regulation of innate immune pathways that will lead to increased understanding of the mechanistic causes of dysregulated IFN that can ultimately result in autoimmune disease. It will also define a new mechanism of immune evasion by flaviviruses that will have implications for therapeutic and vaccine strategies to limit their infection.

抽象的 I 型和 III 型干扰素 (IFN) 限制 RNA 病毒感染。受感染的细胞通过激活的信号产生干扰素通过模式识别受体，如 RIG-I。 RIG-I 和下游信令必须高度协调以获得有效的抗病毒反应。因此，这些过程受到几个翻译后调节修饰（PTM）。这些 PTM 对于 RIG-I 的激活和最终终止至关重要发信号。虽然 RIG-I 信号传导是通过泛素化和磷酸化来协调的，但其机制其他可能调节 RIG-I 信号传导的 PTM（例如泛素样修饰剂）在很大程度上尚不清楚。我们的初步数据显示，称为 ufmylation 的类泛素修饰剂可调节参与 RIG-I 信号传导的多种蛋白质用于响应病毒感染的最佳 IFN 诱导。此外，我们的数据表明 ufmylation 被利用病毒逃避宿主细胞内的先天免疫反应。因此，本提案的目标是确定 ufmylation 如何调节细胞内对病毒感染和病毒逃避的先天免疫反应。根据我们的初步数据，中心假设是 ufmylation 调节宿主和病毒的功能调节抗病毒先天免疫和病毒逃避的蛋白质。根据我们的初步数据，这个假设将通过追求以下三个具体目标进行测试：1) 定义 UFL1（ufmylation E3 连接酶）如何促进RIG-I的激活； 2) 了解关键蛋白质乌酰化的分子机制在 RIG-I 信号通路中下调其功能和信号传导； 3）确定登革热病毒如何合作用于免疫逃避的ufmylation缀合系统。在目标 1 中，UFL1 的分子机制将定义响应RNA病毒感应而诱导RIG-I的激活。在目标 2 中，机制和将确定 ufmylation 如何调节 RIG-I 通路中信号蛋白功能的动态。在目标 3，DENV 蛋白的 ufmy 化促进人体细胞中 DENV 免疫逃避的功能系和原代细胞中，将被确定。总而言之，本申请中提出的工作将是意义重大且具有创新性，因为它将赋予 ufmylation 一种新的免疫调节功能，有助于我们对其基本功能的理解，并揭示了抗病毒先天免疫的新控制（ufmylation）。此外，这项工作将提供对病毒利用宿主定向过程进行免疫的理解。逃避以促进病毒复制。总的来说，这项工作将定义一个新的 PTM 来协调 RIG-I 信号途径，这将提高我们对抗病毒免疫和先天免疫途径调节的了解这将导致人们更加了解干扰素失调的机制原因，最终导致在自身免疫性疾病中。它还将定义黄病毒免疫逃避的新机制，该机制将具有对限制其感染的治疗和疫苗策略的影响。