Histology and Histomorphometry Core

组织学和组织形态计量学核心

• 批准号: 10632033
• 负责人: Patricia Florence Ducy
• 金额: $ 35.2万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-15 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10632033
• 关键词: Aging; Biological; Brain; Bromodeoxyuridine; Cell Count; Cells; Cryoultramicrotomy; Data; Dehydration; Detection; Equipment; Hematoxylin and Eosin Staining Method; Histologic; Histology; Human Resources; Image; In Situ Nick-End Labeling; Medical; Minerals; Mus; Osteogenesis; Pancreas; Paraffin; Proliferating; Research Personnel; Scanning; Skeleton; Specimen; Stains; Stress; Switzerland; System; Tissues; Tolonium chloride; Trichrome stain method; bone; cresyl violet; histological specimens; long bone; microCT; mouse model; programs; sensor; spine bone structure

The four projects of this Program Project heavily rely on the use of mouse models to demonstrate their hypotheses. The purpose of the Histology and Histomorphometry Core is therefore to consolidate equipment and expertise to provide the Investigators of these projects with state-of-the-art, quality controlled histologic and histomorphometric analyses of bone, pancreas and brain specimen as well as microCT of mouse bones. The Core contribution to this Program Project will be at 4 levels: 1. Processing mouse specimens for histology, immunolabeling and histomorphometry. Fixed and partially dehydrated mouse skeletons or pancreata will be embedded in plastic medium (vertebrae and long bones) or paraffin (pancreas). Fixed mouse brain specimen will either be sectioned without embedding or cryo- embedded and cryo-sectioned. 2. Performing specific stainings and immunostainings of mouse tissues sections. Classic histology stainings such as Hematoxylin/Eosin, Toluidine Blue, von Kossa/Van Gieson, Goldner trichrome, Cresyl violet as well as more specific reactive ones such as TUNEL and TRAP stainings will be performed by the Core personnel at the request of investigators. The Core will also perform immunodetection studies using fluorescent and enzymatic detection on sections. 3. Performing histomorphometric analyses of mouse bone, pancreas and brain specimens. The OsteoMeasure Histomorphometry System (OsteoMetrics Inc., Decatur, GA) will be used to assess static parameters, such as bone volume, trabecular number and cell counts, and dynamic parameters of bone formation, such as mineral apposition rates, from non-decalcified bone sections. It will also be used to quantify cell mass/proliferation in pancreas and TUNEL or BrdU positive cells in brain sections. Image J will be used for all other quantifications. The Core will provide investigators with histomorphometric data and will help them in evaluating their biological relevance. 4. Analyzing mouse bone specimen by microCT. Fixed and partially dehydrated mouse long bones and vertebrae will be provided to the Core by project investigators. These specimens will then be scanned using a VivaCT 40 microCT (Scanco Medical, Switzerland).

该程序项目的四个项目在很大程度上依赖于鼠标模型来证明其 假设。因此，组织学和组织学核心的目的是合并设备 和专业知识，可以为这些项目的调查人员提供最先进的质量控制的组织学 骨骼，胰腺和脑标本以及小鼠骨骼的微观分析。 对该计划项目的核心贡献将达到4个级别： 1。用于组织学，免疫标记和组织形态计量学的小鼠标本。固定和部分 脱水的小鼠骨骼或胰腺将嵌入塑料培养基（椎骨和长骨头）中 或石蜡（胰腺）。固定的小鼠脑样本将在不嵌入或冷冻的情况下进行切片 嵌入和冷冻截面。 2。进行小鼠组织切片的特定染色和免疫染色。经典的组织学染色 例如苏木精/曙红，甲甲苯蓝，von Kossa/van Gieson，Goldner Trichrome，Cresyl紫罗兰色为 以及更具体的反应性反应性，例如TUNEL和陷阱染色。 应调查人员的要求。核心还将使用 切片上的荧光和酶促检测。 3。进行小鼠骨，胰腺和脑标本的组织形态计量学分析。这 骨化组织形态测定系统（Osteometrics Inc.，Decatur，GA）将用于评估静态 参数，例如骨体积，小梁数和细胞计数以及骨骼的动态参数 来自未归化的骨切片的形成，例如矿物质的速率。它也将习惯 量化胰腺和TUNEL或BRDU阳性细胞的细胞质量/增殖。图片j Will 用于所有其他量化。核心将为研究人员提供组织形态计量学数据，并 将帮助他们评估其生物学相关性。 4。通过Microct分析小鼠骨标本。固定和部分脱水的小鼠长骨头和 项目调查人员将向核心提供椎骨。这些标本将被扫描 使用Vivact 40 Microct（瑞士Scanco Medical）。