Core B Cellular Core

核心 B 蜂窝核心

• 批准号: 10471371
• 负责人: Andres Finzi
• 金额: $ 9.94万
• 依托单位: DREXEL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10471371
• 关键词: Antibodies; Antigens; CCR5 gene; CD4 Antigens; CXCR4 gene; Cell surface; Cells; Cellular Membrane; Clinical; Complex; Cyclic Peptides; Epitopes; Glycoproteins; Goals; Grant; HIV; HIV Envelope Protein gp120; Immune response; Laboratories; Mediating; Molecular Conformation; Moths; Patients; Resistance; Sampling; Structure; Surface; Ursidae Family; Viral; Virus; antagonist; antibody-dependent cell cytotoxicity; base; gp160; inhibitor; innovation; mimetics; peptidomimetics; programs; response; single-molecule FRET; small molecule; virology; virus envelope

Core B Project Summary: The Goal of the Cellular Core (Core B) is to provide innovation and support to this Program Project to investigate HIV-1 Env antagonism in the context of the conformational states sampled by native HIV-1 Env. Core B will evaluate the impact of the small-molecule antagonists on sensitizing HIV-1-infected cells to antibody-dependent cellular cytotoxicity (ADCC). Core B will also help delineate the mechanisms of action of small-molecule Env inhibitors on trimeric Env expressed at the cell surface. Specifically, Core B will: (a) evaluate the ability of small- molecule Env entry inhibitors for their ability to sensitize HIV-1-infected cells to ADCC and (b) assess their impact on the conformation of functional Env trimers expressed on cell surfaces. Despite not being officially part of the Program Project in the most recent grant period, the Finzi laboratory has been a long-term collaborator and successfully interacted with Virology (Sodroski), Cyclic Peptides and Peptidomimetics (Chaiken), Small Molecule Synthesis (Smith), and Single Molecule FRET (Mothes) Projects to advance the understanding of Env conformational changes at the surface of infected cells, understand how Env conformations are modulated by different small-molecule antagonists generated by the Program Project and assess their impact on Env recognition and ADCC responses mediated by anti-Env antibodies and HIV+ sera.

核心B项目摘要： 细胞核心（核心B）的目标是为该计划项目提供创新和支持 HIV-1 ENV对拮抗作用在由本地HIV-1 Env取样的构象状态的背景下。核心核心 评估小分子拮抗剂对将HIV-1感染细胞敏化对抗体依赖性的影响 细胞细胞毒性（ADCC）。核心B还将帮助描述小分子env的作用机理 在细胞表面表达的三聚体周围的抑制剂。具体而言，核心B将：（a）评估小型的能力 分子ENV进入抑制剂具有使HIV-1感染细胞对ADCC敏感的能力，并且（b）评估其影响 关于在细胞表面上表达的功能env三聚体的构象。尽管没有正式属于 计划项目在最近的赠款期内，Finzi实验室一直是一家长期合作者， 成功与病毒学（Sodroski），环状肽和肽仪（Chaiken），小分子相互作用 综合（史密斯）和单分子fret（mothes）项目促进对env的理解 在受感染细胞表面的构象变化，了解如何调节env构象 计划项目产生的不同的小分子拮抗剂并评估其对Env的影响 抗ENV抗体和HIV+ Sera介导的识别和ADCC反应。