Center for Personal Dynamic Regulomes

个人动态调节中心

• 批准号: 10456125
• 负责人: Howard Y Chang
• 金额: $ 264.19万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10456125
• 关键词: Alleles; Autoimmunity; Automobile Driving; Binding; Biological; Biological Assay; Biology; Biopsy; Biosensor; Blood; Brain; CRISPR interference; Cells; Chromatin; Chromosomes; Clinic; Clinical; Communities; Complex; Coupled; DNA; DNA Sequence; Data; Data Set; Disease; Distant; Dreams; Effectiveness; Elements; Encapsulated; Encyclopedia of DNA Elements; Engineering; Enhancers; Epigenetic Process; Epithelial; Epitopes; Etiology; Event; Exercise; Foundations; Gene Expression Regulation; Generations; Genes; Genetic; Genetic Transcription; Genetic Variation; Genome; Genomics; Genotype; Heterochromatin; Human; Human Genome; Individual; Intervention; Investigation; Knowledge; Laboratories; Lead; Life; Link; Liquid substance; Malignant Neoplasms; Measurement; Methods; Modeling; Modification; Molecular; Molecular Conformation; Multiomic Data; Neurodegenerative Disorders; Organoids; Outcome; Output; Pathology; Patients; Persons; Protein Isoforms; Proteins; RNA; Regulation; Regulator Genes; Regulatory Element; Resolution; Sampling; Schizophrenia; Solid; Source; Speed; Structure; System; Technology; The Cancer Genome Atlas; Time; Tissues; Training; Transact; Untranslated RNA; Variant; base; biological systems; causal variant; cell type; circular RNA; clinical application; computational platform; computerized tools; connectome; disease diagnosis; epigenetic variation; epigenomics; genetic variant; genome-wide; genomic aberrations; human data; human disease; human tissue; improved; individual patient; insight; multiple omics; new technology; novel; personalized medicine; precision medicine; risk variant; sample archive; sample collection; single cell technology; single molecule; transcription factor; treatment response

Project Summary Tens of thousands of human genomes have been sequenced, but the central challenge is their interpretation. A comprehensive set of regulatory events across a genome—the regulome—is needed to make full use of genomic information, but is currently out of reach for most clinical applications and biological systems. The proposed Center will develop technologies that greatly increase the sensitivity, speed, and comprehensiveness of understanding genome regulation. We will develop new technologies to interrogate the transactions between the genome and regulatory factors, such as proteins and noncoding RNAs from single cells, and integrate variations in DNA sequences and chromatin states over time and across individuals. Novel molecular engineering and biosensor strategies are deployed to encapsulate the desired complex DNA transformations into the probe system, such that the probe system can be directly used on very small human clinical samples and capture genome-wide information in one or two steps. These technologies will be applied to clinical samples with genomic aberrations to exercise their robustness, and reveal for the first time epigenomic dynamics of human diseases during progression and treatment. These technologies will be broadly applicable to many biomedical investigations, and the Center will disseminate the technologies via training and diverse means.

项目摘要 已经对成千上万的人类基因组进行了测序，但是中心挑战 是他们的解释。跨基因组的一系列全面的监管事件 - 调节 - 需要充分利用基因组信息，但目前不在 达到大多数临床应用和生物系统。拟议的中心将 开发大大提高灵敏度，速度和的技术 了解基因组调节的全面性。我们将开发新的 询问基因组和调节因素之间交易的技术， 例如蛋白质和来自单细胞的非编码RNA，以及DNA中的整合变化 序列和染色质随着时间的流逝和跨个体的状态。新分子 部署工程和生物传感器策略以封装所需的复合物 DNA转换为探针系统，以便可以直接进行探针系统 用于一个很小的人类临床样本并捕获全基因组信息 或两个步骤。这些技术将应用于具有基因组的临床样本 畸变以行使其稳健性，并首次揭示表观基因组学 人类疾病进展和治疗过程中的动力学。这些技术会 广泛适用于许多生物医学研究，中心将传播 通过培训和潜水员的技术手段。