Core--Mouse Molecular Genetics

核心--小鼠分子遗传学

• 批准号: 6989483
• 负责人: ROBERT GLEN OSHIMA
• 金额: $ 18.38万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-22 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6989483
• 关键词: animal colony; binding proteins; biological signal transduction; biomedical facility; breast neoplasms; developmental genetics; fibroblasts; genetically modified animals; laboratory mouse; liver neoplasms; molecular genetics; molecular oncology; neoplasm /cancer genetics; oncogenes; pancreas neoplasms; prostate neoplasms; protein structure function; stem cells; tissue /cell culture; tissue resource /registry

The Mouse Molecular Genetics Core will support the components of this Program to facilitate the investigation of the developmental, physiological, and pathological roles of the signaling molecules Bit1, DOCK180, Gab1, and SHEP1. The conditional knockout mouse models generated by each laboratory will be tested for extraembryonic tissue function during development. In addition, each will be tested with appropriate transgenic mouse tumor models. Using a newly developed model based on the K18 gene and the Polyoma Middle T antigen (PyMT) oncogene, the roles of the Bit1, DOCK180, Gab1, and SHEP1 genes in the development of mammary tumors, hepatomas, pancreatic tumors, and prostate neoplasms will be evaluated. In addition, this Core facility will provide investigators with trophoblast stem cells, embryonic fibroblasts, and tumor cells for additional experiments. Finally, this Core will assist and train investigators in embryo development and additional genetic manipulations of their respective genes.

小鼠分子遗传学核心将支持该计划的各个组成部分，以促进 研究信号分子 Bit1、DOCK180、Gab1 和 SHEP1 的发育、生理和病理作用。每个实验室生成的条件敲除小鼠模型将在发育过程中进行胚胎外组织功能测试。此外，每一种都将用适当的转基因小鼠肿瘤模型进行测试。使用基于 K18 基因和多瘤中 T 抗原 (PyMT) 癌基因的新开发模型，Bit1、DOCK180、Gab1 和 SHEP1 基因在乳腺肿瘤、肝癌、胰腺肿瘤和前列腺肿瘤发展中的作用将进行评估。此外，该核心设施还将为研究人员提供滋养层干细胞、胚胎成纤维细胞和肿瘤细胞，以进行其他实验。最后，该核心将协助和培训研究人员进行胚胎发育和各自基因的额外遗传操作。