Microbial and Phage Cultivation Core

微生物和噬菌体培养核心

基本信息

批准号：
10357966
负责人：
ROBERT A BRITTON
金额：
$ 40.04万
依托单位：
BAYLOR COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-03-01 至 2026-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10357966
关键词：
Anaerobic Bacteria Animal Disease Models Anti-Bacterial Agents Antibiotic Resistance Bacteria Bacterial Antibiotic Resistance Bacteriophages Clostridium difficile Collection Communities Complex Disease ESKAPE pathogens Etiology Evolution Faculty Foundations Generations Genes Genome Genomics Goals Health Homeostasis Human Human Microbiome Human Resources Human body Individual Infrastructure Intestines Investments Laboratories Libraries Lytic Medicine Metagenomics Microbe Mission Mucous Membrane Nature Nose Organoids Pathogenicity Production Reagent Research Resources Role Scientist Sequence Analysis Services Site Surface Therapeutic Vagina Viral atmospheric conditions college combat cost data integration deep sequencing gene function host microbiome human model innovation metagenome metagenomic sequencing microbial microbial community microbial genome microbiome microbiome alteration microbiome research novel pathogen pathogenic bacteria tool translational study

项目摘要

PROJECT SUMMARY The overall goal of the BCM-CARBIRU is to explore non-antibiotic therapeutic approaches to combating antibiotic resistant bacterial pathogens that are deemed urgent and serious threats. To meet this goal, the establishment of the Microbial and Phage Cultivation (MPC) core will provide to the projects the ability to cultivate bacteria and phage in multiple formats and under different atmospheric conditions. The core will also provide sequencing support of newly identified bacteria and phage that emerge from each of the three projects. The MPC organized around three primary objectives. Objective 1. Cultivation of complex microbial communities and individual microbes. Objective 2. Isolation and optimization of bacteriophage for AMR pathogens. Objective 3. Sequencing of individual microbes, phage and complex microbial communities. Centralization of these objectives into the MPC core will reduce costs by allowing all of the projects access to the infrastructure (such as anaerobic and microoxic chambers), bulk purchasing of reagents, and the reduction in labor costs by having dedicated technicians to assist in generation of bacteria and phage. The MPC will facilitate the transfer of expertise in microbial and phage cultivation from the core to trainees from all three projects. Finally, a centralized resource for sequencing of bacteria and phage will allow more coordination between the projects in regard to genomic analysis and data integration. The MPC will be an essential part of the BCM-CARBIRU that, along with the Organoid Cultivation Core, will help achieve the overall goals proposed.

项目概要 BCM-CARBIRU 的总体目标是探索非抗生素治疗方法对抗被视为紧急和严重威胁的抗生素耐药细菌病原体。到为了实现这一目标，微生物和噬菌体培养（MPC）核心的建立将提供该项目具有以多种形式和在不同条件下培养细菌和噬菌体的能力大气条件。该核心还将为新发现的细菌提供测序支持以及从这三个项目中出现的噬菌体。 MPC 围绕三个主要组织目标。目标1.复杂微生物群落和微生物个体的培养。目的2.AMR病原体噬菌体的分离和优化。目标 3. 对单个微生物、噬菌体和复杂微生物群落进行测序。将这些目标集中到 MPC 核心将通过允许所有项目来降低成本使用基础设施（例如厌氧室和微氧室），批量采购试剂，并通过专门的技术人员协助生成来降低劳动力成本细菌和噬菌体。 MPC 将促进微生物和噬菌体专业知识的转让从三个项目的核心到学员的培养。最后，集中资源细菌和噬菌体的测序将使项目之间在以下方面进行更多协调基因组分析和数据整合。 MPC 将成为 BCM-CARBIRU 的重要组成部分，与类器官培养核心一起，将有助于实现所提出的总体目标。