Impact of microbiota-mediated biotransformation of black tea polyphenols

微生物介导的红茶多酚生物转化的影响

基本信息

批准号：
9398095
负责人：
Christian Jobin
金额：
$ 41.28万
依托单位：
UNIVERSITY OF FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-01-01 至 2019-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9398095
关键词：
Accounting Acids Address Animals Anti-inflammatory Apoptosis Bacteria Beverages Bilateral Biological Biological Availability Biological Process Biota Black Tea Cancer Etiology Catechin Cell Proliferation Cessation of life Chemopreventive Agent Colitis associated colorectal cancer Colonic Neoplasms Colorectal Cancer Complex Consumption Data Development Diagnosis Diet Drug Kinetics Economic Burden Enterococcus faecalis Environmental Risk Factor Escherichia coli Excretory function Food Gallic acid Generations Germ-Free Gnotobiotic Goals Health Health Benefit Heart Diseases Hour House mice In Vitro Individual Inflammatory Bowel Diseases Intestines Knowledge Location Malignant Neoplasms Measures Mediating Metabolic Metabolic Biotransformation Metabolism Microbe Modeling Molecular Weight Mono-S Mucous Membrane Mus Oral Organ Organism Pathology Patients Plasma Play Polymers Positioning Attribute Preventive Process Production Reporting Resources Ribosomal RNA Role Sampling Signal Transduction System Tea Theaflavins Therapeutic Tissues United States Urine Wild Type Mouse Woman absorption cancer prevention colon cancer cell line commensal bacteria cost dimer enzyme activity expectation germ free condition gut microbiota human microbiota improved inflammatory marker insight intestinal homeostasis liquid chromatography mass spectrometry medical complication men metabolomics microbial microbial community microbiome research microbiota microorganism novel phenolic acid polyphenol prevent public health relevance therapeutic target transcriptome sequencing

项目摘要

DESCRIPTION (provided by applicant): Tea is one of the most widely consumed beverages in the world, with black tea accounting for 78% of the production. Consumption of tea has been associated with many health benefits including the prevention of cancer and heart disease. These effects have been attributed to the polyphenol compounds present in tea. Numerous studies have shown that tea polyphenols including catechins, their dimers (theaflavins) and polymers (thearubigins), have anti-inflammatory and cancer preventive activities. However, theaflavins and thearubigins, the major polyphenols in black tea, have poor systematic bioavailability. Therefore, it is still unclear how these compounds could exert their biological functions. Studies have shown that higher molecular weight polyphenols are metabolized by the microbiota and their metabolites may play an important role in black tea chemopreventive action. Ring-fission products and lower molecular weight phenolic acids have been identified as the major bacterial metabolites of catechins. However, the bacterial metabolites of theaflavins and thearubigins are still unknown. Our preliminary data indicate that microbial-derived metabolites are generated from the tea polyphenol thearubigins. Our central hypothesis is that the microbiota influence the generation of black tea polyphenol-derived metabolites enhancing their bioavailability and biological activities. The goal of this project is to determine the crosstalk between bacteria and polyphenol metabolism and establish the functional consequences on development of colitis-associated colorectal cancer. Our specific aims are as follows: 1) Determine the impact of the selective microbiota on biotransformation and pharmacokinetics of black tea polyphenol-derived metabolites. 2) Determine the relationship between microbial-derived metabolites and the biological action of black tea polyphenols/metabolites in colitis-associated colorectal cancer. 3) Measure the impact of black tea polyphenols on microbial community composition and activities. This project will determine the impact of the microbiota on black tea polyphenol- mediated biological effect in Il10-/- mice. Since the microbial community is variable among individuals, our findings will provide novel insight into how the biological activity of a defined dietary product could be altered by microorganisms. This knowledge could be utilized to improve biotransformation of active food products and to enhance their biological activity in various organs including the intestine. In addition, the basic knowledge gained from the interplay between microorganisms and dietary-derived metabolite productions could uncover novel enzymes activity and therapeutic targets.

描述（由申请人提供）：茶是世界上消费最广泛的饮料之一，其中红茶占产量的 78%。茶的消费与许多健康益处有关，包括预防癌症和心脏病。这些作用归因于茶叶中的多酚化合物，大量研究表明，茶多酚，包括儿茶素、其二聚体（茶黄素）和聚合物（茶红素），具有抗炎和抗癌作用。然而，红茶中的主要多酚茶黄素和茶红素的系统生物利用度较差，因此，目前尚不清楚这些化合物如何通过微生物群代谢并发挥其生物学功能。它们的代谢物可能在红茶化学预防作用中发挥重要作用，环裂变产物和较低分子量的酚酸已被确定为儿茶素的主要细菌代谢物。然而，茶黄素和茶红素的细菌代谢物仍然未知。我们的初步数据表明，微生物衍生的代谢物是由茶多酚茶红素产生的。我们的中心假设是，微生物群影响红茶多酚衍生代谢物的产生，从而增强其生物利用度。该项目的目标是确定细菌和多酚代谢之间的相互作用，并确定对结肠炎相关结直肠癌发展的功能影响。具体目标如下： 1) 确定选择性微生物群对红茶多酚衍生代谢物的生物转化和药代动力学的影响 2) 确定微生物衍生代谢物与红茶多酚/代谢物在结肠炎中的生物作用之间的关系。 3) 测量红茶多酚对微生物群落组成和活动的影响该项目将确定微生物群对红茶的影响。 Il10-/- 小鼠中多酚介导的生物效应由于个体之间的微生物群落不同，我们的研究结果将为微生物如何发挥特定饮食产品的生物活性提供新的见解。活性食品并增强其在包括肠道在内的各个器官中的生物活性。从微生物和膳食衍生代谢产物之间的相互作用中获得的知识可以揭示新的酶活性和治疗靶点。