CORE--TRANSGENIC ANIMALS

核心——转基因动物

• 批准号: 3737216
• 负责人: JEFFREY A WHITSETT
• 金额: --
• 依托单位: CINCINNATI CHILDRENS HOSP MED CTR
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3737216
• 关键词: animal breeding; athymic mouse; biomedical facility; cell line; chloride channels; cystic fibrosis; gene targeting; genetically modified animals; laboratory mouse; lung disorder; mutant

Rationale. The gene therapy program is highly dependent upon the generation and maintenance of transgenic and hybrid transgenic mice for the study of cystic fibrosis and other diseases. This core is designed to take advantage of established expertise in the generation of the transgenic mice for the study of lung disease and in gene ablation studies that will be required for the present program projects. Likewise, maintenance of germ-free transgenic lines will be critical in supplying genotyped mice for the various projects. This core will both generate the new transgenic lines and gene targeted mice, as well as maintain breeding and genotype analysis for the transgenic mice already generated, as required for the individual projects and investigators. This Core will: 1) provide animal husbandry, breeding, tail clip, Southern blot, PCR analysis for the established lines necessary for the present application; 2) generate all new transgenic lines described in the protocols by pronuclear injection and gene targeting; 3) facilitate long term maintenance and surveillance of viral exposed animals required for the various projects. The animals will be maintained in sterile- barrier caging in a separate, germ-free facility at the Children's Hospital Research Foundation under the direction of Gary Keller, D.V.M. This program includes sentinel mice and careful ongoing surveillance and screening for other pathogens. This aspect of Core will interact closely with the Clinical Viral Core for vector safety issues. Such surveillance will be critical in the evaluation of lung disease in our various transgenic models and be necessary for the interpretation of genetic vs. environmental effects on lung injury or development.

理由。 基因疗法计划高度依赖 转基因和杂种转基因小鼠的生成和维护 囊性纤维化和其他疾病的研究。 这个核心是设计的 利用建立的专业知识 转基因小鼠研究肺部疾病和基因消融 本计划项目将需要的研究。 同样，无细菌转基因线的维护至关重要 为各种项目提供基因分型小鼠。 这个核心将俩都 生成新的转基因线和基因靶向小鼠，以及 已经维持已转基因小鼠的育种和基因型分析 根据各个项目和调查人员的要求生成。 该核心将：1）提供畜牧业，育种，尾夹， Southern印迹，PCR分析的既定线 目前的申请； 2）生成所有描述的新的转基因线 原核注射和基因靶向的方案； 3）便利 需要长期维持和对病毒暴露动物的监视 对于各种项目。 动物将在无菌中维持 - 在儿童的一个单独的，无菌的设施中，屏障笼 医院研究基金会在加里·凯勒（Gary Keller）的指导下 该程序包括前哨小鼠和仔细的持续监视和 筛查其他病原体。 核心的这一方面将密切相互作用 与临床病毒核心有关媒介安全问题。 这样的监视 在我们的各种评估中对肺部疾病的评估至关重要 转基因模型，对于解释遗传VS是必要的。 环境对肺损伤或发育的影响。