Cell Function Analysis Core

细胞功能分析核心

• 批准号: 10311498
• 负责人: Sakeneh Zraika
• 金额: $ 30万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-02-10 至 2022-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10311498
• 关键词: Adipocytes; Affect; Animals; Brain; Calcium; Catheters; Cell Survival; Cell physiology; Cells; Chronic; Closure by clamp; Collection; Conscious; Consultations; Detection; Diabetes Mellitus; Disease; Endothelial Cells; Funding; Glucose Clamp; Goals; Harvest; Hepatocyte; Hormones; Human; Hydrogen Peroxide; Hyperglycemia; Hypoglycemia; In Vitro; Islet Cell; Islets of Langerhans Transplantation; Liver; Lymph; Lymphocyte; Manuscripts; Measurement; Membrane Potentials; Metabolism; Mitochondria; Morphology; Muscle satellite cell; Neurons; Obesity; Oxygen Consumption; Peer Review Grants; Peptides; Process; Production; Proteomics; Publications; Regulation; Research; Research Personnel; Research Support; Retina; Rodent; Rodent Model; Role; Services; Signal Transduction; Skeletal Muscle; Superoxides; System; Testing; Time; Tissues; Training; Universities; Washington; analytical tool; base; cell type; cytochrome c; glucose metabolism; glucose tolerance; in vivo; insulin tolerance; islet; macrophage; metabolic abnormality assessment; metabolic phenotype; minimally invasive; mitochondrial membrane; response; temporal measurement

The overall objective of the Cell Function Analysis Core at the University of Washington Diabetes Research Center is to provide affiliates with analyses of glucose metabolism, mitochondrial function and intracellular signaling to support research of diabetes, obesity and related disorders. To achieve this goal, the Core aims to: (1) Provide real time functional analysis using in vitro flow culture systems of tissues/cells; (2) Provide in vivo assessments of metabolic phenotypes in rodent models important in diabetes research; (3) Provide static assessment of cellular metabolism and function; (4) Harvest, isolate and culture primary tissue from rodents, including islets and islet cells, liver, retina and brain, for subsequent morphological and functional characterization, as well as to procure human islets for the same purposes; (5) Offer training and consultation to affiliates, their trainees and staff; and (6) Develop new analytical tools requested by affiliates to support their studies of the metabolic regulation of cell function as it relates to research in diabetes, obesity and related disorders. Since inception of the Core in 2002, in vitro analysis has been the major focus. Cell and tissue types that have been analyzed include islets, retina, skeletal muscle, stem cells, macrophages, lymphocytes, adipocytes, endothelial cells, neuronal cells and liver/hepatocytes. Recently, in vivo services have been added to combine both the detailed and mechanistic analyses provided in cell and tissue studies with the ability to test the roles of identified processes in whole body settings. Whole animal studies currently offered include glucose and insulin tolerance tests, hyperinsulinemic-euglycemic clamps, islet transplantation and collection of lymph. Expansion of new in vitro, ex vivo, and in vivo services has allowed the Core to better serve the needs of the Center's research base. As diabetes affects metabolism and signaling in many cell types, the services of the Cell Function Analysis Core continue to be of great value to many Center affiliate investigators. The Core plans to continue to provide users with systematic and integrated approaches to the analysis of cell types that are critically involved in diabetes and its complications, obesity and related disorders.

华盛顿大学糖尿病研究的细胞功能分析核心的总体目标 中心将为会员提供葡萄糖代谢，线粒体功能和细胞内的分析 信号以支持糖尿病，肥胖和相关疾病的研究。为了实现这一目标，核心的目的是： （1）使用组织/细胞的体外流动培养系统提供实时功能分析； （2）提供体内 评估在糖尿病研究中重要的啮齿动物模型中代谢表型的评估； （3）提供静态 评估细胞代谢和功能； （4）从啮齿动物那里收获，分离和培养原代组织， 包括胰岛和胰岛细胞，肝脏，视网膜和大脑，用于随后的形态和功能 表征以及为同一目的采购人类胰岛； （5）提供培训和咨询 致分支机构，他们的学员和员工； （6）开发分支机构要求支持其的新分析工具 与糖尿病，肥胖和相关研究有关的细胞功能的代谢调节的研究 疾病。 自2002年核心成立以来，体外分析一直是主要重点。细胞和组织类型 已经分析的包括胰岛，视网膜，骨骼肌，干细胞，巨噬细胞，淋巴细胞，脂肪细胞， 内皮细胞，神经元细胞和肝/肝细胞。最近，添加了体内服务以组合 细胞和组织研究中提供的详细和机械分析，具有测试作用的能力 在整个身体设置中确定的过程。目前提供的全动物研究包括葡萄糖和 胰岛素耐受性测试，高胰岛素 - 糖血糖夹，胰岛移植和淋巴的收集。 扩展新体外，Ex Vivo和体内服务使核心能够更好地满足其需求 中心的研究基础。由于糖尿病会影响许多细胞类型的新陈代谢和信号传导，因此 细胞功能分析核心对许多中心会员研究人员仍然具有很大的价值。核心计划 继续为用户提供系统和集成的方法，以分析细胞类型 批判性地参与糖尿病及其并发症，肥胖和相关疾病。