Analytical - Core B

分析 - 核心 B

• 批准号: 9096154
• 负责人: J Thomas Cunningham
• 金额: $ 30.6万
• 依托单位: UNIVERSITY OF NORTH TEXAS HLTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-05 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9096154
• 关键词: Accounting; Anatomy; Antibodies; Area; Biochemical; Biological Assay; Brain region; Cardiovascular system; Chronic; Core Facility; Coupled; Data Analyses; Data Files; Databases; Drug Compounding; Equipment; Functional disorder; Gene Proteins; Genomics; Harvest; Health Sciences; Human Resources; Hypoxia; Immunohistochemistry; Instruction; Label; Mass Spectrum Analysis; Methods; Mission; Neurons; Peptides; Physiology; Population; Post-Translational Protein Processing; Preparation; Procedures; Process; Proteins; Proteomics; RNA; Reagent; Reporter; Research; Research Assistant; Research Institute; Research Personnel; Resources; Sampling; Schedule; Secure; Site; Sleep Apnea Syndromes; Specialist; System; Technical Expertise; Tissue Sample; Western Blotting; Work; abstracting; brain cell; cost; insight; instructor; interest; laser capture microdissection; professor; research study; sample collection

Abstract – Core B This core focuses on qualitative and quantitative analyses of genes, proteins, peptides and small drug compounds using conventional biochemical methods coupled with highly sensitive mass spectrometry (MS) and Laser Capture Microdissection (LCM) for sample collection. It will serve as a central resource for LCM and qRT-PCR (UNTHSC) and MS analysis of covalent protein modifications (UTHSCSA) of CNS samples from the 3 projects. The core will receive raw samples from investigators, most often these will be in the form of punches from specific brain regions of interest. LCM will be used to harvest defined populations of cells from brain sections allowing the projects to conduct experiments using samples that are more specific than punched samples. Retrograde track tracing, juxtacellular labeling, immunohistochemistry, and the expression of reporter molecules will be used to identify neurons of interest.

摘要 – 核心 B 该核心重点关注基因、蛋白质、肽和小药物的定性和定量分析 使用传统生化方法结合灵敏的高质谱 (MS) 分析化合物 以及用于样品采集的激光捕获显微切割 (LCM)，它将作为 LCM 和激光捕获显微切割 (LCM) 的中心资源。 对来自中枢神经系统样本的共价蛋白修饰 (UTHSCSA) 进行 qRT-PCR (UNTHSC) 和 MS 分析 3 个项目的核心将从调查人员那里收到原始样本，最常见的是以下形式： 来自特定感兴趣的大脑区域的冲击将用于从特定的细胞群中收获。 大脑切片允许项目使用比打孔更具体的样本进行实验 样本逆行追踪、细胞旁标记、免疫组织化学和报告基因的表达。 分子将用于识别感兴趣的神经元。