Ixodes scapularis and Amblyomma americanum tick cement proteome

肩胛硬蜱和美洲钝蜱蜱水泥蛋白质组

• 批准号: 9321502
• 负责人: ALBERT MULENGA
• 金额: $ 1.28万
• 依托单位: TEXAS A&M AGRILIFE RESEARCH
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-03-15 至 2018-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9321502
• 关键词: Adhesives; Amblyomma; Anaplasma phagocytophilum; Animals; Arthropod Vectors; Babesia microti; Biopsy; Bite; Black-legged Tick; Blood; Borrelia burgdorferi; Collaborations; Cone; Containment; Data; Deposition; Development; Disease; Dissection; Event; Future; Glues; Goals; Grooming; Health; Human; Ixodidae; Lasers; Lesion; Letters; Lyme Disease; Methods; Physiology; Process; Proteins; Proteome; Protocols documentation; Public Health; RNA Interference; Rickettsia rickettsii; Sequence Analysis; Site; Skin; Tick Control; Tick-Borne Diseases; Ticks; Time; United States; Vector-transmitted infectious disease; acaricide; base; combinatorial; comparative; feeding; human disease; improved; novel; prevent; programs; protein function; success; sucking; transmission process

 DESCRIPTION (provided by applicant): We propose to identify and characterize proteins in innermost layer of Ixodes scapularis and Amblyomma americanum tick cement cones. Currently there are 14 reportable human tick borne disease (TBD) agents are recognized in the United States with 13 of these transmitted via hard tick bites (http://www.cdc.gov/ticks/diseases/). Hard tick species are adapted to feed for many days. Majority of hard ticks secrete an adhesive substance known as "tick cement" that allows ticks to firmly anchor onto host skin, and prevent them from being groomed off their feeding site by their host. The tick cement cone has two layers: the innermost layer, which is deposited within 5-30min of the tick starting the feeding process, and the outermost layer, which begins to be deposited one day after the tick has started the feeding process. Deposition of the innermost layer precedes major tick feeding events including transmission of major human tick borne diseases such as the Lyme disease agent. Thus, our assumptions are that preventing functions of proteins that form the innermost layer of the tick cement cone will stop tick feeding before transmission of major human disease agents starts. There are two specific aims in this application. First is to identify proteins in I. scapularis and A. americanum tick cement cones, and second is to verify significance in tick attachment and/or completion of tick feeding. Of the 14 reportable human TBD agents, in the United States, a combined 9 are vectored by I. scapularis and A. americanum (http://www.cdc.gov/ticks/diseases/). Thus proposed discovery of I. scapularis and A. americanum tick cement proteins will have broader impacts toward development of novel methods to improve public health in the USA.

 描述（由申请人提供）：我们建议鉴定并表征肩胛硬蜱和美洲钝蜱蜱水泥锥最内层的蛋白质。目前，美国已识别出 14 种可报告的人类蜱传疾病 (TBD) 病原体，其中 13 种。通过硬蜱叮咬传播（http://www.cdc.gov/ticks/diseases/）。大多数硬蜱物种可以进食很多天。硬蜱会分泌一种称为“蜱粘固剂”的粘性物质，使蜱虫能够牢固地固定在宿主皮肤上，并防止它们被宿主梳理掉其进食部位。蜱粘固剂锥有两层：最内层，最内层。蜱开始进食过程后 5-30 分钟内沉积，最外层在蜱开始进食过程一天后开始沉积，最内层的沉积先于主要蜱进食事件，包括主要人类传播。打钩因此，我们的假设是，在主要人类疾病媒介开始传播之前，阻止形成蜱水泥锥最内层的蛋白质的功能将停止蜱的进食。该应用有两个具体目标。首先是鉴定 I 中的蛋白质。 第二个目的是验证蜱附着和/或蜱进食完成的重要性，在美国，在 14 种可报告的人类 TBD 病原体中，共有 9 种是由肩胛蜱和美洲蜱传播的。因此，提出的 I. scapularis 和 A. americanum 蜱水泥蛋白的发现将具有更广泛的用途。对开发改善美国公共卫生的新方法的影响。