Ixodes scapularis and Amblyomma americanum tick cement proteome

肩胛硬蜱和美洲钝蜱蜱水泥蛋白质组

• 批准号: 9321502
• 负责人: ALBERT MULENGA
• 金额: $ 1.28万
• 依托单位: TEXAS A&M AGRILIFE RESEARCH
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-03-15 至 2018-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9321502
• 关键词: Adhesives; Amblyomma; Anaplasma phagocytophilum; Animals; Arthropod Vectors; Babesia microti; Biopsy; Bite; Black-legged Tick; Blood; Borrelia burgdorferi; Collaborations; Cone; Containment; Data; Deposition; Development; Disease; Dissection; Event; Future; Glues; Goals; Grooming; Health; Human; Ixodidae; Lasers; Lesion; Letters; Lyme Disease; Methods; Physiology; Process; Proteins; Proteome; Protocols documentation; Public Health; RNA Interference; Rickettsia rickettsii; Sequence Analysis; Site; Skin; Tick Control; Tick-Borne Diseases; Ticks; Time; United States; Vector-transmitted infectious disease; acaricide; base; combinatorial; comparative; feeding; human disease; improved; novel; prevent; programs; protein function; success; sucking; transmission process

 DESCRIPTION (provided by applicant): We propose to identify and characterize proteins in innermost layer of Ixodes scapularis and Amblyomma americanum tick cement cones. Currently there are 14 reportable human tick borne disease (TBD) agents are recognized in the United States with 13 of these transmitted via hard tick bites (http://www.cdc.gov/ticks/diseases/). Hard tick species are adapted to feed for many days. Majority of hard ticks secrete an adhesive substance known as "tick cement" that allows ticks to firmly anchor onto host skin, and prevent them from being groomed off their feeding site by their host. The tick cement cone has two layers: the innermost layer, which is deposited within 5-30min of the tick starting the feeding process, and the outermost layer, which begins to be deposited one day after the tick has started the feeding process. Deposition of the innermost layer precedes major tick feeding events including transmission of major human tick borne diseases such as the Lyme disease agent. Thus, our assumptions are that preventing functions of proteins that form the innermost layer of the tick cement cone will stop tick feeding before transmission of major human disease agents starts. There are two specific aims in this application. First is to identify proteins in I. scapularis and A. americanum tick cement cones, and second is to verify significance in tick attachment and/or completion of tick feeding. Of the 14 reportable human TBD agents, in the United States, a combined 9 are vectored by I. scapularis and A. americanum (http://www.cdc.gov/ticks/diseases/). Thus proposed discovery of I. scapularis and A. americanum tick cement proteins will have broader impacts toward development of novel methods to improve public health in the USA.

 描述（由适用提供）：我们建议在ixodes肩cap骨和Amblyomma Americanum Tick水泥锥中识别和表征蛋白质。目前，在美国认可了14种可报告的人tick虫疾病（TBD）特工，其中13个通过硬滴答叮咬传输（http://www.cdc.gov/ticks/diseases/）。硬滴答物种适应了很多天的饲料。大多数硬tick秘密秘密一种被称为“ tick水泥”的粘合剂物质，该物质可以首先锚定在宿主皮肤上，并防止其宿主将其修饰。 Tick水泥锥有两层：最内层的层，该层沉积在滴答物的5-30分钟内，开始喂食过程，最外层的一层开始在壁虱开始喂食过程后一天开始存放。最内层的沉积之前的主要滴答喂食事件包括传播人类tick虫出生疾病，例如莱姆病毒剂。因此，我们的假设是防止构成tick水泥锥体最内层的蛋白质功能将在传播主要人类疾病药物开始之前停止进食。该应用程序中有两个具体的目标。首先是识别I中的蛋白质。 肩cap骨和A. Americanum Tick水泥，其次是验证tick附件和/或完成滴答喂食的意义。在美国的14个可报告的人类TBD代理中，有一个合计的9个由I. scapularis和A. amerivemum tick水泥蛋白进行了介绍，将对开发新方法产生更大的影响，以改善美国的公共卫生。