Effects of Acupuncture on Bone Cancer Pain in Mice

针刺对小鼠骨癌疼痛的影响

基本信息

批准号：
6775585
负责人：
RUIXIN ZHANG
金额：
$ 18.56万
依托单位：
UNIVERSITY OF MARYLAND BALTIMORE
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-08-01 至 2005-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6775585
关键词：
acupuncture /acupressure behavior test bone neoplasms cancer pain disease /disorder model dynorphins electrostimulus femur fos protein heat stimulus histology hyperalgesia immunocytochemistry laboratory mouse neoplasm /cancer palliative treatment neurons nonhuman therapy evaluation spinal cord

项目摘要

DESCRIPTION (provided by applicant): Cancer pain, particularly bone cancer pain, is a major symptom that significantly affects the quality of life of cancer patients. Clinical trials have demonstrated that acupuncture can alleviate cancer pain, but as yet evidence from basic scientific research is absent. An animal model of femur bone cancer pain, which mimics clinical cancer pain, has been established in our lab. We propose to use this model to evaluate the effects and mechanisms of electroacupuncture (EA) at acupoint G30 (Huan Tiao) on femoral cancer pain. The proposed hypotheses are: 1) EA of acupoint G30 at the optimal sets of parameters will significantly inhibit femur bone cancer-induced hyperalgesia. 2) Activity of the central neurons during cancer pain will be modulated by EA. The specific aims of this proposal are: Aim I: Evaluate the effects of EA on cancer-induced thermal hyperalgesia by measuring hindpaw withdrawal latency. Various combinations of frequencies (10 and 100 Hz) and stimulation durations (10, 15, 20 min) at the maximum tolerable electrical current of 1 mA will be administered to study the anti-hyperalgesic effects of EA in a mouse model of bone cancer pain. Aim II- Determine the optimal treatment protocol. With the optimal sets of parameters determined in Aim I, various EA treatment regimens will be conducted to establish how often treatments should be administered to achieve the greatest anti-hyperalgesic effects. Aim lll: Investigate the central mechanisms of action of EA on cancer pain. The modulation of EA on the activities of central neurons in the spinal cord will be investigated by characterizing Fos protein and dynorphin peptide expression with immunohistochemical staining. These studies will advance our knowledge of EA in control and management of cancer pain and provide useful information for designing future clinical trials using optimal EA parameters for patients with cancer pain. These studies will set a stage for further studying the mechanisms of EA in the control and management of cancer pain.

描述（申请人提供）：癌痛，特别是骨癌痛，是严重影响癌症患者生活质量的主要症状。临床试验已经证明针灸可以减轻癌症疼痛，但目前还缺乏基础科学研究的证据。我们实验室建立了模拟临床癌痛的股骨癌痛动物模型。我们建议使用该模型来评估电针（EA）G30（还条）穴位对股骨癌疼痛的作用和机制。提出的假设是：1）在最佳参数组下对G30穴位进行电针将显着抑制股骨癌引起的痛觉过敏。 2) 癌痛期间中枢神经元的活动将受到电针的调节。该提案的具体目标是：目标 I：通过测量后爪缩回潜伏期来评估 EA 对癌症引起的热痛觉过敏的影响。将在最大可耐受电流 1 mA 下进行频率（10 和 100 Hz）和刺激持续时间（10、15、20 分钟）的各种组合，以研究 EA 在骨癌疼痛小鼠模型中的抗痛觉过敏作用。目标 II - 确定最佳治疗方案。根据目标 I 中确定的最佳参数集，将进行各种 EA 治疗方案，以确定应该进行治疗的频率，以实现最大的抗痛觉过敏效果。目标三：研究 EA 对癌性疼痛的主要作用机制。将通过免疫组织化学染色表征 Fos 蛋白和强啡肽表达来研究 EA 对脊髓中枢神经元活动的调节。这些研究将增进我们对 EA 在控制和管理癌痛方面的了解，并为设计未来使用最佳 EA 参数治疗癌痛患者的临床试验提供有用的信息。这些研究将为进一步研究电针控制和治疗癌痛的机制奠定基础。