Clemson Light Imaging Facility (CLIF)

克莱姆森光学成像设备 (CLIF)

基本信息

批准号：
8813282
负责人：
Terri Lane Foster Bruce
金额：
$ 19.18万
依托单位：
CLEMSON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8813282
关键词：
Address Biochemical Biological Sciences Cell Count Cell Separation Cell physiology Cells Centers of Research Excellence Charge Communities Conceptions Core Facility Cytometry Data Development Educational workshop Equipment Faculty Fees Floor Flow Cytometry Fluorescence-Activated Cell Sorting Fostering Funding Generations Goals Grant Guava Fruit Housing Image Imagery Imaging Techniques Institution Laboratories Light Light Microscope Location Microscope Microscopy Multiphoton Fluorescence Microscopy Organelles Preparation Research Research Infrastructure Research Personnel Research Project Grants Research Technics Resolution Running Sorting - Cell Movement South Carolina Specimen Students Targeted Research Technical Expertise Techniques Technology Time Training Training Support United States National Institutes of Health Universities Work base collaborative environment design equipment training fluorescence activated cell sorter device graduate student imaging system improved innovation instrumentation interest light microscopy meetings member pathogen polarized light research study tool university student user-friendly

项目摘要

Summary: The Clemson Light Imaging Facility (CLIF) will serve as the Imaging and Cell Sorting Core for the EPIC COBRE. The CLIF is a multi-user core facility located in the new Clemson University Life Sciences Facility. The core is under the direction of Dr. Terri Bruce, and houses a suite of Nikon microscopes, a Zeiss LSM 510 confocal microscope, a CytoViva Hyperspectral Imaging System, a Leica DM 750 P polarized light microscope, a Leica DM IL inverted contrasting microscope, a Leica M80 stereomicroscope, a Leica TCS SPE spectral confocal and a Leica SP8X multiphoton spectral confocal microscope. Additionally, the CLIF houses cell counting and sorting equipment, including a Guava EasyCyte HT and a BD Influx FACS cell sorter. The CLIF includes a multi-user specimen preparation laboratory, and a dedicated 20-seat classroom equipped with video conferencing and recording capabilities. The specific aims of the Imaging and Cell Sorting Core are 1) to enhance the cell sorting capabilities of the Clemson Light Imaging Facility (CLIF); 2) to promote and facilitate the research undertaken by the target PIs of this COBRE; and 3) to promote and facilitate research at Clemson University and the state of South Carolina. Aim 1 will be addressed by the addition of a BioRad S3 Cell Sorter (funds from CU Provost) and a dedicated cell sorting technician. Aims 2&3 will be addressed through providing assistance to target investigators with design and implementation of visualization and cell sorting experiments, providing advanced technique and equipment training, and providing organized workshops on multiphoton/confocal imaging and advanced cell sorting.

摘要：Clemson Light Imaging设施（CLIF）将作为成像和细胞分类核心史诗般的鞋底。 CLIF是位于新克莱姆森大学生活科学的多用户核心设施设施。核心位于特里·布鲁斯（Terri Bruce）博士的指导下，并设有一套尼康显微镜 LSM 510共聚焦显微镜，cytoviva高光谱成像系统，Leica DM 750 P极化光显微镜，Leica DM IL倒对比显微镜，Leica M80立体显微镜，Leica TCS SPE 光谱共聚焦和Leica SP8X多极子光谱共聚焦显微镜。此外，Clif房屋细胞计数和分类设备，包括Guava Eastcyte HT和BD涌入FACS细胞分选仪。这 CLIF包括一个多用户标本准备实验室，以及配备20个座位的专用教室视频会议和录制功能。成像和细胞排序核心的具体目的是1）增强细胞分类功能 Clemson Light Imaging设施（CLIF）； 2）促进和促进目标PI进行的研究这个毛刺; 3）促进和促进克莱姆森大学和南卡罗来纳州的研究。 AIM 1将通过添加Biorad S3细胞分系师（来自Cu Provost的资金）和专用细胞分类技术员。目标2和3将通过向目标调查员提供帮助可视化和细胞分类实验的设计和实施，提供高级技术和设备培训，并提供有关多光子/共聚焦成像和高级单元格的有组织研讨会排序。