Serial, non-invasive molecular analysis of exhaled breath condensate to define the pulmonary flora in critically injured, ventilated adults

对呼出气冷凝物进行连续、非侵入性分子分析，以确定重伤、通气成人的肺部菌群

• 批准号: 9473059
• 负责人: Wonder P. Drake
• 金额: $ 31.21万
• 依托单位: VANDERBILT UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-15 至 2021-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9473059
• 关键词: Accounting; Adult; Antibiotic Therapy; Antibiotics; Bacterial DNA; Benchmarking; Bronchoalveolar Lavage; Bronchoalveolar Lavage Fluid; Chest; Clinical; Clinical Management; Communicable Diseases; Coughing; Critical Care; Critical Illness; DNA; Data; Development; Diagnosis; Early Diagnosis; Early identification; Environmental air flow; Equilibrium; Exhalation; Fever; Functional disorder; Goals; Growth; Hour; Immune response; Infection; Intensive Care Units; Length of Stay; Leukocytosis; Liquid substance; Lung; Lung infections; Manuscripts; Measures; Mechanical ventilation; Mechanics; Microbial Genetics; Molecular; Molecular Analysis; Morbidity - disease rate; Nature; Non-Invasive Cancer Detection; Oxygen; Patient-Focused Outcomes; Patients; Pneumonia; Predisposition; Procedures; Property; Pulmonary alveolar structure; Recombinant DNA; Regression Analysis; Research; Resolution; Risk; Sampling; Severities; Signs and Symptoms; Specimen; Sputum; Symptoms; Testing; Therapeutic; Thoracic Radiography; Time; Trauma; Trauma patient; Tube; Universities; Virulence; Work; aerosolized; antimicrobial; base; clinical Diagnosis; cohort; cost; diagnosis standard; endotracheal; healthcare-associated infections; injured; innovation; lung microbiota; microbial community; mortality; novel; pathogen; predictive modeling; public health relevance; response; targeted delivery; tool; treatment response; ventilator-associated pneumonia

DESCRIPTION (provided by applicant): This is an innovative proposal to determine the capacity of molecular analysis of exhaled breath condensate filters (EBCF) to predict ventilator associated pneumonia (VAP) in trauma intensive care unit patients. Based upon preliminary data of 51 patients suspected of having VAP, who underwent bronchoalveolar lavage, we have previously demonstrated a strong correlation between bronchoalveolar lavage and breath condensate quantitative PCR. The goal of this proposal is to understand the changes that occur in the pulmonary microbial community of mechanically ventilated, critically injured adults to support the development of molecular analysis of EBCF to provide an earlier, more sensitive and specific diagnosis of VAP, to enable: 1) earlier recognition of a developing pneumonia, 2) targeted antimicrobial therapy, and 3) assessment of treatment response.

描述（由申请人提供）：这是一项创新建议，旨在确定呼出呼吸冷凝物过滤器（EBCF）分子分析以预测创伤重症监护病人患者中相关肺炎（VAP）的能力。基于51名涉嫌进行VAP的患者的初步数据，他们接受了支气管肺泡灌洗，我们以前已经证明了支气管肺泡灌洗与呼吸凝结物定量PCR之间存在很强的相关性。该提案的目的是了解机械通风，严重受伤的成年人的肺微生物群落中发生的变化，以支持EBCF分子分析的发展，以提供早期，更敏感和更具体的VAP诊断，以启用：1 ）早期识别发生的肺炎，2）靶向抗菌治疗和3）评估治疗反应。