Small and large animal surgery, physiology and histology

小型和大型动物外科、生理学和组织学

基本信息

批准号：
9357852
负责人：
John William Elrod
金额：
$ 57.24万
依托单位：
TEMPLE UNIV OF THE COMMONWEALTH
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9357852
关键词：
Anatomy Animal Model Animals Area Balloon Angioplasty Biochemical Biological Assay Blinded Cardiac Cell Therapy Cells Clinic Collaborations Consultations Coronary artery Coupling Custom Data Data Analyses Evaluation Experimental Designs Family suidae Fostering Functional disorder Goals Gold Heart Diseases Histologic Histology Human Injury Investigation Ischemia Left Ligation Mediator of activation protein Methodology Miniature Swine Modeling Molecular Molecular Mechanisms of Action Mus Myocardial Myocardial Infarction Myocardial Ischemia Myocardial dysfunction Myocardium Nodal Operative Surgical Procedures Pathogenesis Pathology Patients Phenotype Physiology Population Process Productivity Program Development Program Research Project Grants Protocols documentation Reperfusion Injury Reperfusion Therapy Reproducibility Research Design Research Infrastructure Research Personnel Resources Services Signal Transduction Stem cells Structure Surgical Models Techniques Testing Therapeutic Translations Treatment Efficacy Variant Vertebral column collaborative environment exosome experience experimental study in vivo in vivo Model injury and repair mouse model novel paracrine pre-clinical programs repaired success synergism treatment strategy

项目摘要

PROJECT SUMMARY The Cardiac Physiology and Surgical Core (Core C) will be a focal point of the program by providing the infrastructure to support numerous experimental approaches to assess cardiac physiology and pathology, in the intact animal. Utilizing the vast experience and expertise of the Co-Director's, and well developed and proven protocols for the assessment of cardioprotection in multiple animal models, the Core will not only provide a consistent and reproducible service in support of the projects but will further participate in experimental design, prioritization of resources, and the interpretation of data. In addition, Core C will identify additional areas for collaboration across all projects as the program matures and thus be central to the interactive environment and comprehensive analysis of the overall platform as data is generated. The scientific core will provide a wide-range of services pertinent to the overall success of the program including in both mouse and porcine models: delivery of novel cell-free exosome therapeutics to the myocardium, in vivo induction of ischemia/reperfusion (IR) injury, the assessment of cardiac structure and function by multiple methodologies, and histological and biochemical assessment of myocardial injury. These services will provide the backbone for the major focus of this Program Project Grant to determine the impact of exosome therapy on the molecular pathogenesis of cardiac IR-injury.

项目摘要心脏生理和手术核心（核心C）将通过提供该计划的焦点基础设施支持众多实验方法来评估心脏生理和病理学，完整的动物。利用联合导演的丰富经验和专业知识，并发达了，在多种动物模型中评估心脏保护的经过验证的协议，核心不仅将提供一致且可重复的服务以支持项目，但将进一步参与实验设计，资源的优先级以及数据解释。此外，核心C将确定随着计划的成熟，在所有项目中进行协作的其他领域，因此是核心随着数据的产生，交互式环境和整体平台的全面分析。这科学核心将提供与该计划的整体成功有关的广泛服务在小鼠和猪模型中：新颖的无细胞外泌体治疗剂向心肌递送体内诱导缺血/再灌注（IR）损伤，心脏结构和功能的评估方法论以及心肌损伤的组织学和生化评估。这些服务将提供该计划项目赠款的主要重点的骨干，以确定外部疗法的影响关于心脏IR受伤的分子发病机理。