COMPARATIVE ANIMAL MODELS CORE

比较动物模型核心

基本信息

批准号：
9276038
负责人：
Charles G Wray
金额：
$ 15.73万
依托单位：
MOUNT DESERT ISLAND BIOLOGICAL LAB
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至 2018-06-14
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9276038
关键词：
Address Aging Amphibia Animal Experimentation Animal Model Animals Behavioral Biological Models Biological Process Biology Biology of Aging Biomedical Research Caenorhabditis elegans Cell Culture Techniques Centers of Research Excellence Collaborations Comparative Biology Comparative Study Complement Complex Embryo Faculty Fishes Fundulus Future Genotype Goals Growth Instruction Invertebrates Longevity Maine Maintenance Mammalian Cell Marines Medicine Methods Microinjections Modeling Molecular Mus Natural regeneration Nature Organ Organism Pharmacotherapy Predisposition Process Quarantine Recording of previous events Research Research Infrastructure Research Personnel Resources Scientist Sea Urchins Services Speed The Jackson Laboratory Tissue Harvesting Transgenic Organisms Visit Visual Xenopus Yin Zebrafish animal facility animal resource base comparative cost experimental study human disease in vivo innovation insight marine organism regenerative square foot success surgical service tissue regeneration tissue repair

项目摘要

PROJECT SUMMARY (See instructions): Robust regeneration of tissues and complex organs is the norm for countless invertebrate and nonmammalian vertebrate species. In addition, nature has endowed certain animals with remarkable longevity and robust tissue repair mechanisms that slow aging-induced degenerative processes, or with increased susceptibility to cellular and molecular damage and concomitantly short lifespans. Numerous fundamental questions in regenerative biology can only be answered in vivo using diverse non-mammalian animal models. In addition, comparative studies of diverse organisms provide deeper mechanistic insights that are not possible using a single model or "model-centric" approach, and they can speed the pace and reduce the cost of biomedical discovery. The Comparative Animal Models Core builds on MDIBL's unique strength and expertise in comparative animal biology and will provide COBRE investigators with resources necessary to utilize diverse animal models in regenerative and aging biology research. For the current application, the Core focuses primarily on providing resources and services to COBRE Project Leaders for their proposed zebrafish studies. These resources include day-to-day husbandry, importation and quarantine services, spawning services to produce embryos for experimental studies, microinjection and surgical services, drug treatment services and genotyping using visual inspection and PCR-based methods. The Core will also have both the facilities and expertise necessary to provide COBRE associated investigators as well as potential new COBRE Project Leaders with resources for other vertebrate and invertebrate model systems useful in regenerative and aging biology research including C. elegans, amphibians and various fish species. Our long term goal is to develop the Core into a one-of-a-kind animal resource by leveraging the scientific background and expertise of the Core Director, his staff and MDIBL's faculty, as well as MDIBL's 113-year history of providing and maintaining diverse marine organisms for biomedical research. The Comparative Animal Models Core will thus contribute not only to the success and long term sustainability of the COBRE, but also to the success and continued growth of MDIBL and biomedical research in Maine.

项目摘要（参见说明）：组织和复杂器官的强大再生是无数无脊椎动物和非哺乳动物脊椎动物物种的常态。此外，大自然赋予某些动物显着的长寿和强大的组织修复机制，可以减缓衰老引起的退化过程，或者对细胞和分子损伤的敏感性增加，从而缩短寿命。再生生物学中的许多基本问题只能使用不同的非哺乳动物动物模型在体内得到解答。此外，对不同生物体的比较研究提供了更深入的机制见解，这是使用单一模型或“以模型为中心”的方法不可能实现的，并且它们可以加快生物医学发现的速度并降低成本。比较动物模型核心建立在 MDIBL 在比较动物生物学方面的独特优势和专业知识的基础上，将为 COBRE 研究人员提供在再生和衰老生物学研究中利用不同动物模型所需的资源。对于当前的应用程序，核心主要侧重于为 COBRE 项目负责人提出的斑马鱼研究提供资源和服务。这些资源包括日常饲养、进口和检疫服务、为实验研究生产胚胎的产卵服务、显微注射和外科服务、药物治疗服务以及使用目视检查和基于 PCR 的方法进行基因分型。该核心还将拥有必要的设施和专业知识，为 COBRE 相关研究人员以及潜在的新 COBRE 项目负责人提供可用于再生和衰老生物学研究的其他脊椎动物和无脊椎动物模型系统的资源，包括线虫、两栖动物和各种鱼类。我们的长期目标是利用核心主任、其工作人员和 MDIBL 教师的科学背景和专业知识，以及 MDIBL 113 年提供和维护动物资源的历史，将核心发展成为独一无二的动物资源。用于生物医学研究的多种海洋生物。因此，比较动物模型核心不仅将有助于 COBRE 的成功和长期可持续性，而且还将有助于缅因州 MDIBL 和生物医学研究的成功和持续发展。