Mechanism of Candida albicans rupture on biomimetic NSS

仿生NSS上白色念珠菌破裂的机制

• 批准号: 9377579
• 负责人: DENNIS R LA JEUNESSE
• 金额: $ 43.64万
• 依托单位: UNIVERSITY OF NORTH CAROLINA GREENSBORO
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2022-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9377579
• 关键词: Adhesions; Alpha Cell; Antibiotic Resistance; Antibiotics; Antifungal Agents; Bacterial Infections; Biological Assay; Biomimetics; Candida albicans; Candidiasis; Cell Adhesion; Cell Wall; Cells; Cellular Morphology; Cellular Structures; Chemicals; Communities; Community Outreach; Confocal Microscopy; Development; Drug resistance; Effectiveness; Environment; Festival; Fungal Drug Resistance; Goals; Growth; Health; Home environment; Hospitals; Human; Immunocompromised Host; Industry; Infection; Joints; Knowledge; Mechanics; Microbe; Microbial Biofilms; Microfluidics; Minority-Serving Institution; Mission; Morbidity - disease rate; Mutation; Mycoses; Nanostructures; North Carolina; Pathogenicity; Play; Research; Research Personnel; Resistance; Resistance development; Role; Rupture; Scanning Electron Microscopy; Schools; Science; Science, Technology, Engineering and Mathematics Education; Structure; Surface; Training; Training and Education; Transplant Recipients; Underserved Population; Universities; Work; Yeasts; antimicrobial; assault; design; doctoral student; extracellular; fungus; insight; killings; methicillin resistant Staphylococcus aureus; microbial; mortality; nanoengineering; nanomaterials; nanoscale; nanoscience; nanostructured; novel; outreach program; programs; resistant strain; response; student training; undergraduate student

Project Summary/Abstract Project Title: Mechanism of Candida albicans rupture on biomimetic NSS PI: Dennis LaJeunesse (UNCG/Nanoscience) The goals of this research are to characterize the nanoscale mechanical interactions between a pathogenic yeast cell, Candida albicans and a cell-rupturing nanostructured surface (NSS) and to define the conditions that determine and control NSS-induced yeast cell rupture. Our long term goals involve the application of NSS’s as a mechanical means of controlling pathogenic fungal growth and biofilm formation. In this proposed research we have three aims: 1) characterize the interactions of C. albicans with a rupturing NSS and determine the roles that cell morphology and adhesion play in NSS induced cell rupture; 2) perform a structural and chemical analysis of the yeast cell during NSS rupture to determine what aspects of the C. albicans cell are vulnerable to NSS assault; and 3) determine how drug resistance strains of C. albicans respond to NSS assault. The proposed research will be the first systematic study of the interactions of a human pathogenic fungi C. albicans and a NSS. Antimicrobial NSS used alone or in conjunction with traditional antifungal drugs have tremendous potential impact for novel antimicrobial treatments. This work will provide greater insight into the fundamental mechanisms of NSS-induced response and practical knowledge that will facilitate the application of NSS fungal control. In addition to the importance of the proposed research, this work will also contribute significantly the central North Carolina Piedmont community as well. The Joint School of Nanoscience and Nanoengineering has a mission to train students, attract industry, and engage the community of the central North Carolina Piedmont. The work will involve the training of doctoral students and several undergraduate researchers in essential STEM programs and will be a major component of community outreach programs such as the JSNN science festival and Nanobus. The University of North Carolina at Greensboro is a minority serving institution and thus this research will also serve to promote STEM education and training to an underserved population.

项目摘要/摘要 项目名称：白色念珠菌破裂的机理在仿生NSS上 PI：Dennis Lajeunesse（UNCG/纳米科学） 这项研究的目标是表征纳米级机械相互作用 在致病性酵母细胞，白色念珠菌和细胞破裂的纳米结构之间 表面（NSS）并定义确定和控制NSS诱导的酵母的条件 细胞破裂。我们的长期目标涉及NSS作为机械手段的应用 控制致病真菌生长和生物膜形成。在这项拟议的研究中 我们有三个目标：1）表征白色念珠菌与破裂NSS的相互作用 并确定细胞形态和粘合剂在NSS诱导的细胞中的作用 破裂; 2）在NSS破裂期间对酵母细胞进行结构和化学分析 确定白色念珠菌细胞的哪些方面容易受到NSS攻击的影响； 3） 确定白色念珠菌的耐药性菌株如何应对NSS攻击。这 拟议的研究将是对人类相互作用的首次系统研究 致病真菌白色念珠菌和NSS。单独或共同使用的抗菌NSS 传统的抗真菌药物对新型抗菌素具有巨大的潜在影响 治疗。这项工作将为您提供更深入的了解 NSS引起的响应和实践知识将有助于NSS的应用 真菌控制。除了拟议的研究的重要性外，这项工作还将 北卡罗来纳州皮埃蒙特社区也有显着贡献。关节 纳米科学和纳米工程学校的使命是培训学生，吸引力 行业，并吸引北卡罗来纳州中部皮埃蒙特社区。工作 将涉及博士生和几位本科研究人员的培训 基本的STEM计划，将是社区外展的主要组成部分 诸如JSNN科学节和纳米布斯之类的节目。北大学 格林斯伯勒的卡罗来纳州是少数派服务机构，因此这项研究也将 用于促进对服务不足的人群的STEM教育和培训。