Pattern formation and regeneration in a single cell

单细胞中的模式形成和再生

基本信息

批准号：
9206510
负责人：
Wallace Marshall
金额：
$ 41.37万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-02-01 至 2019-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9206510
关键词：
Animal Model Animals Anterior Bioinformatics Biological Models Biology Candidate Disease Gene Cell division Cells Cellular Structures Complex Cytoskeleton Development Developmental Biology Dissection Embryo Embryology Excision Face Failure Fluorescent in Situ Hybridization Gene Expression Genes Genetic Transcription Genome Genomics Giant Cells Individual Maps Messenger RNA Methods Microtubules Molecular Molecular Biology Morphogenesis Morphology Motor Natural regeneration Nuclear Operative Surgical Procedures Oral Organism Pathway interactions Pattern Pattern Formation Phenotype Plants Play Positioning Attribute Process RNA Interference RNA-Binding Proteins Reproducibility Role Shapes Signal Pathway Signal Transduction Structure Surface Testing Time Transcript Work Wound Healing base cell cortex course development gene function genomic tools molecular modeling morphogens programs public health relevance single molecule tool transcriptome sequencing

项目摘要

DESCRIPTION (provided by applicant): Morphogenesis and pattern formation are vital processes in any organism, whether unicellular or multicellular. But in contrast to the developmental biology of plants and animals, the principles of morphogenesis and pattern formation in single cells remain largely unknown. Although all cells develop patterns, the giant ciliate Stentor coeruleus provides unique features of large size, highly reproducible patterns and cellular landmarks, and a remarkable ability to heal wounds that allows microsurgical approaches of a type normally seen in embryology. Stentor was a popular model organism for exploring the mechanism of cellular morphogenesis and regeneration but was never developed as a molecular model system. We have now sequenced the Stentor genome and developed a method for testing gene function by RNAi. We propose to use these genomic tools to test a set of hypothesis about how the cell cortex and nuclear work together to direct pattern formation during cell development and allow regeneration of cellular structures following surgical removal. We expect our studies to open a new window onto the mechanisms by which cells form patterns and develop complex structures, which is a universal problem throughout all of biology.

描述（由适用提供）：形态发生和模式形成是任何组织中的重要过程，无论是单细胞还是多细胞。但是，与动植物的发育生物学相反，单细胞中形态发生和模式形成的原理在很大程度上仍然未知。尽管所有细胞都会发展出模式，但巨型纤毛固定层提供了较大尺寸，高度可重复的模式和细胞地标的独特特征，以及具有出色的健康胜利能力，可以使胚胎学中通常看到的类型的显微外科手术方法。 Stentor是一种流行的模型生物体，用于探索细胞形态发生和再生的机理，但从未作为分子模型系统开发。现在，我们已经测序了Stentor基因组，并开发了一种通过RNAi测试基因功能的方法。我们建议使用这些基因组工具来测试一组关于细胞皮层和核如何共同起作用在细胞发育过程中形成的假设，并允许手术去除后细胞结构的再生。我们希望我们的研究能为细胞形成模式并发展复杂结构的机制打开一个新窗口，这是整个生物学的普遍问题。