Cell Cycle proteomicsin Xenopus

非洲爪蟾细胞周期蛋白质组学

基本信息

批准号：
9319402
负责人：
MARC Wallace KIRSCHNER
金额：
$ 20.33万
依托单位：
HARVARD MEDICAL SCHOOL
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-07-01 至 2017-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9319402
关键词：
Affect Algorithms Biochemical Biochemistry Bioinformatics Biology Cell Cycle Cell Cycle Regulation Cell Nucleus Cell physiology Cells Characteristics Communities Complex Cytoplasm Cytoskeleton Data Databases Development Dose Drug Design Drug Targeting Embryo Embryonic Development Event Experimental Designs Fertilization Genetic Transcription Health Human Image Inflammation Investigation Knowledge Label Learning Life Malignant Neoplasms Mass Spectrum Analysis Measures Meiosis Methods Methylation Microtubules Modification Oocytes Pathway interactions Peptides Pharmaceutical Preparations Phosphorylation Site Post-Translational Protein Processing Preparation Protein Dynamics Proteins Proteolysis Proteome Proteomics Qualifying Reaction Reaction Time Reading Regulation Research Resolution Role Sampling Site System Techniques Technology Time Titrations Translations Ubiquitination Xenopus Xenopus Proteins Yeasts base blastomere structure data mining deep sequencing egg gastrulation genetic regulatory protein improved interest protein complex research study small molecule inhibitor tandem mass spectrometry tool wiki zygote

项目摘要

DESCRIPTION (provided by applicant): Proteomics has special significance for Xenopus research because key events in meiosis, fertilization and early development are regulated at the level of translation, post- translational modification and proteolysis, and not by transcription. Proteomics is the only tool for systematically investigating these levels of regulation and discovering new mechanisms. We propose to develop a cutting edge proteomic research platform for Xenopus embryos and egg extract by migrating technology developed in human and yeast systems, and to publicly disseminate the databases and tools we develop. We will also optimize methods for proteomic analysis of protein complexes and compartments in egg extract. Using these tools we will quantify relative amounts of >5,000 proteins, >10,000 phosphorylation sites and >1,500 ubiquitination sites in early development and in egg extracts under normal and perturbed conditions. We will investigate the mechanism of cell cycle regulation, how the cell cycle changes during early development, how microtubules are nucleated, and how embryos detect changes in the nucleus to cytoplasm ratio.

描述（由申请人提供）：蛋白质组学对于爪蟾研究具有特殊意义，因为减数分裂、受精和早期发育中的关键事件是在翻译、翻译后修饰和蛋白水解水平上调节的，而不是通过转录调节的。蛋白质组学是系统研究这些调控水平和发现新机制的唯一工具。我们建议通过迁移在人类和酵母系统中开发的技术，开发非洲爪蟾胚胎和卵提取物的尖端蛋白质组学研究平台，并公开传播我们开发的数据库和工具。我们还将优化鸡蛋提取物中蛋白质复合物和区室的蛋白质组学分析方法。使用这些工具，我们将量化正常和扰动条件下早期发育和鸡蛋提取物中> 5,000个蛋白质、> 10,000个磷酸化位点和> 1,500个泛素化位点的相对量。我们将研究细胞周期调控机制、细胞周期在早期发育过程中如何变化、微管如何成核以及胚胎如何检测细胞核与细胞质比率的变化。