Core B - Mutagenesis and cell expression core

核心 B - 诱变和细胞表达核心

• 批准号: 9792295
• 负责人: Carlos G. Vanoye
• 金额: $ 45.76万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9792295
• 关键词: Adopted; Alternative Splicing; Brain; CRISPR/Cas technology; Cell Line; Cells; Communities; Complementary DNA; Cryopreservation; Cultured Cells; Electrophysiology (science); Electroporation; Engineering; Ensure; Epilepsy; Evaluation; Exhibits; Functional disorder; Future; Generations; Genome; Human; Institutes; Ion Channel; Laboratories; Methods; Molecular; Mus; Mutagenesis; Mutant Strains Mice; Mutation; Neonatal; Neurobiology; Neurons; Pattern; Pharmacology; Pharmacology Study; Phenotype; Plasmids; Point Mutation; Potassium; Protein Isoforms; Proteins; RNA Splicing; Recombinant DNA; Recombinants; Research; Services; Site; Site-Directed Mutagenesis; Sodium; Standardization; System; Technology; Time; Transfection; Universities; Variant; Vendor; Work; cellular engineering; cost; cost effective; design and construction; early onset; genetic variant; genome editing; human model; innovative technologies; interest; mouse model; novel; patch clamp; programs; promoter; stable cell line; success; vector; voltage

PROJECT SUMMARY – Core B Our Center proposes to study a large number of previously uncharacterized epilepsy-associated genetic variants in human ion channels using a high-throughput strategy (Project 1). We also propose to generate novel mouse models of prototypical variants after we demonstrate that mutant mouse ion channels exhibit patterns of dysfunction similar to the corresponding human variant (Project 3). Both of these research endeavors will rely heavily upon the engineering of variants in recombinant ion channels and the generation of expression systems in heterologous cultured cells. In addition, Project 3 requires the design and construction of targeting vectors for generation of novel mouse models using genome editing technologies. The Mutagenesis and Cell Expression Core (Core B) will be responsible for these two activities. To accomplish this work in an efficient, cost-effective, and rigorous manner, Core B will exploit standardized high-throughput workflows for site-directed mutagenesis and heterologous cell transfection that unite innovative technologies with economies of scale. The specific variants to be generated will be determined by the Variant Prioritization and Curation Core (Core A). Service activities will be distributed between the Translational Neurobiology program at the Broad Institute of Harvard University and MIT and the Department of Pharmacology at Northwestern University to take advantage of advanced recombinant DNA and cell engineering expertise on these two campuses. Core B will provide the following services: 1) to engineer recombinant human ion channel plasmids and variants for functional evaluation; 2) to generate and distribute heterologous cells transiently or stably expressing human ion channel variants; and 3) to generate and heterologously express prototypical mouse ion channel variants for comparison with the corresponding human variants and to construct murine targeting vectors.

项目摘要 – 核心 B 我们中心提议研究大量以前未表征的癫痫相关遗传 我们还建议使用高通量策略生成人类离子通道的变体（项目 1）。 在我们证明突变小鼠离子通道表现出原型变异的新型小鼠模型 功能障碍模式与相应的人类变体相似（项目 3）。 努力将在很大程度上依赖于重组离子通道变体的工程设计以及 此外，项目3需要设计和构建异源培养细胞中的表达系统。 使用基因组编辑技术生成新型小鼠模型的靶向载体。 诱变和细胞表达核心（核心 B）将负责这两项活动。 为了以高效、经济且严格的方式完成这项工作，Core B 将利用标准化 用于定点诱变和异源细胞转染的高通量工作流程 具有规模经济的创新技术将由以下因素决定。 变体优先级划分和管理核心（核心 A）活动将在 哈佛大学和麻省理工学院博德研究所的转化神经生物学项目及其系 西北大学药理学系利用先进的重组 DNA 和细胞技术 这两个校区的工程专业知识将提供以下服务：1) 进行工程设计。 用于功能评估的重组人离子通道质粒和变体；2) 生成和分发 瞬时或稳定表达人离子通道变体的异源细胞；以及3)产生和 异源表达原型小鼠离子通道变体，以便与相应的人类离子通道进行比较 变体并构建小鼠靶向载体。