Biodegradable Polymer Surgical Sealant with Increased Adhesion and Reduced Inflammation

可生物降解的聚合物手术密封剂，具有增强的粘附力和减少的炎症

• 批准号: 9794002
• 负责人: John Louis Daristotle
• 金额: $ 1.17万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-21 至 2020-01-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9794002
• 关键词: Acute; Address; Adhesions; Adhesives; Adsorption; Anastomosis - action; Animal Model; Award; Biocompatible Materials; Biodegradation; Biological; Biology; Biomaterials Research; Body Temperature; Career Choice; Cecum; Cell Adhesion; Cell Survival; Cells; Chemistry; Clinic; Clinical; Complement; Complication; Data; Deposition; Devices; Diffuse; Diffusion; Elastomers; Ethylene Oxide; Fellowship; Fiber; Fibrin Tissue Adhesive; Fluorescence-Activated Cell Sorting; Foreign Bodies; Geometry; Glycolates; Goals; Grant; Hemostatic function; Hour; Hydration status; Hydrophobicity; Immunohistochemistry; Implant; In Vitro; Infection; Inflammation; Inflammatory; Inflammatory Response; Intestinal Motility; Intestines; Investigation; Liquid substance; Materials Testing; Methods; Modeling; Mus; National Institute of Biomedical Imaging and Bioengineering; Operating Rooms; Operative Surgical Procedures; Outcome; Particle Size; Polymers; Preparation; Procedures; Property; Proteins; Research; Safety; Scanning Electron Microscopy; Science; Site; Surface; Surgical complication; Surgical incisions; Surgical sutures; Survival Rate; Technical Expertise; Techniques; Testing; Tissue Adhesives; Tissue Engineering; Tissues; Toxic effect; Training; Translating; Water; Work; adhesive polymer; amphiphilicity; base; biodegradable polymer; biomaterial compatibility; caprolactone; copolymer; cost; design; ethylene glycol; functional group; hydrophilicity; implant material; improved; in vivo; insight; interfacial; intraperitoneal; materials science; mortality; mouse model; nanoparticle; nanoscale; particle; poly(lactide); poly(propylene oxide); portability; pre-doctoral; preclinical trial; pressure; prevent; response; scaffold; seal; small molecule; success; usability

Project Summary This project’s objective is to develop a sprayable, biodegradable polymer surgical sealant for decreasing high mortality rate complications that occur after surgery. Surgical sealants currently available in the clinic have high cost, poor material properties, and are difficult to precisely deposit. Utilizing a technique called solution blow spinning, we have demonstrated the ability to directly deposit conformal biodegradable polymer fiber surgical sealants to the site of surgery in vivo with exceptional sealing strength. The overall hypothesis for this research is that reducing inflammation and increasing tissue adhesion of polymer surgical sealants will allow us to demonstrate success in pre-clinical trials for reducing complication rate after an intestinal anastomosis. This project will investigate sprayable combinations of biodegradable polymers such as poly(lactic-co-glycolic acid) (PLGA) or poly(lactide-co-caprolactone), poly(ethylene glycol) (PEG), and functionalized nanoparticles that can reduce inflammation or increase wet tissue adhesion. The Specific Aims of the proposed research are: (1) Reduce inflammation in response to biodegradable polymers by controlling hydrophilicity using block copolymer additives. (2) Evaluate intestinal function and complication rate for PLGA-PEG surgical sealants in vivo. (3) Increase wet tissue adhesion using functionalized nanoparticles that increase physical bonding at the interface. The insights produced by these studies will also be applicable to other polymeric biomedical devices, such as sutures and tissue engineering scaffolds, increasing the significance of the work. This proposal has been designed to complement a fellowship training plan that develops cross-disciplinary technical skills in materials science and biology, leading to an academic career path in biomaterials research.

项目摘要 该项目的目标是开发可喷涂的可生物降解聚合物手术密封剂，以降低高 手术后发生的死亡率并发症。目前在诊所可用的手术密封剂很高 成本，材料特性不佳，难以精确沉积。利用一种称为溶液打击的技术 旋转，我们已经证明了直接沉积共形生物降解聚合物纤维手术的能力 在体内手术部位的密封剂具有特殊的密封强度。这项研究的总体假设 是减少炎症和增加的聚合物手术密封剂的组织粘附，将使我们得以 在临床前试验中表现出成功，以降低肠道吻合后的并发症率。这 项目将研究可生物降解的聚合物（例如聚乳乙酸）的可喷涂组合 （PLGA）或聚（乳酸 - 二苯乙烯），聚（乙二醇）（PEG）和功能化的纳米颗粒，可以 减少炎症或增加湿组织粘附。拟议研究的具体目的是：（1） 通过使用块共聚物控制亲水性，减少对可生物降解聚合物的炎症 添加剂。 （2）评估体内PLGA-PEG手术密封剂的肠功能和并发症率。 （3） 使用功能化的纳米颗粒增加湿组织粘合剂，从而增加界面处的物理键合。 这些研究产生的见解也将适用于其他聚合物生物医学设备，例如 缝合线和组织工程支架，增加了工作的重要性。这个建议已经 旨在补充奖学金培训计划，以发展材料的跨学科技术技能 科学与生物学，导致生物材料研究的学术职业道路。