ShEEP Request for Chromium System

ShEEP 对 Chromium 系统的请求

• 批准号: 9796800
• 负责人: Salman Azhar
• 金额: --
• 依托单位: VETERANS ADMIN PALO ALTO HEALTH CARE SYS
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9796800
• 关键词: Affect; Aging; Autoimmune Diseases; Biological; Biological Process; Biology; Blood Vessels; Bone Diseases; Cancer Model; Cells; Chromium; Computer software; DNA; DNA analysis; Data; Degenerative polyarthritis; Development; Disease; Disease model; Encapsulated; Event; Gel; Gene Expression; Generations; Genes; Genome; Genomic DNA; Genomics; Gulf War; Haplotypes; Health; Healthcare; Heterogeneity; Human; Human Genome; Immune; Immune response; Individual; Injury; Knowledge; Label; Libraries; Link; Metabolic Diseases; Metabolic syndrome; Mission; Molecular; Molecular Weight; Muscle; Nerve Degeneration; Obesity; Oils; Pain; Pathogenesis; Pathologic; Patient Care; Phase; Population; Preparation; Process; Radiation; Reagent; Research; Research Personnel; Rodent; Rotavirus Infections; Sampling; Sheep; Stem cells; Steroid biosynthesis; Structure; System; T cell regulation; T-Cell Activation; Technology; Variant; Veterans; Whole-Genome Shotgun Sequencing; Work; adipokines; artery stenosis; bone healing; cell type; design; exome; genome analysis; health administration; improved; innovation; insertion/deletion mutation; instrument; lipid metabolism; lipid transport; nanolitre; non-alcoholic fatty liver disease; research study; targeted treatment; wound

The 10x Genomics Chromium System is an important instrument for our research. The 10X Genomics Chromium System is comprised of the Chromium Controller, Chromium i7 Multiplex kit, Chromium Single Cell 3' Library and Gel Bead Kit, and the Chromium Single Cell A Chip kit. These components are used together to perform Genome “linked read” library preps and sequencing. These components will only work with the Chromium Controller and cannot be used independently. There are many investigators that are researching specific single cell types to elucidate mechanisms of action of various biological processes. This is hard to do in primary cells as there are many cell types that must be isolated from one another. This system can identify single cells, so different cell populations can be studied without other cell types confounding the results. This Single Cell and DNA Analysis 10X Genomics™ consists of 10x GemCode™ that fuels Chromium System with an innovative reagent delivery of previously inaccessible genomic information at a massive rate and scale. The Chromium Controller can quickly encapsulate individual cells or genomic DNA into 100,000s to 1,000,000s of uniquely addressable partitions in minutes, each containing an identifying barcode for downstream genomic analysis with the Chromium Software Suite. It will allow us to explore how aging and other diseases conditions impact major classes of structural variants (SVs) like deletions, inversions and translocations, and also allows the sequencing of genes inaccessible to short-read sequencers. 10X Genomics™ will also allow analysis of Phase SNVs, indels and SVs across > 10 Mb haplotype blocks. Finally, it will allow generation of high-quality libraries from as little as 1 ng of genomic DNA. This technology is extremely important to VA research. There are fourteen principle investigators at VAPAHCS who will apply this technology in their research to analyze gene expression at a single cell level to elucidate mechanisms of aging, steroidogenesis, wound/bone healing, lipid metabolism, cellular immune responses, T cell activation, bone diseases, obesity, Gulf War Illness, as well as many other metabolic diseases and conditions that particularly affect the Veterans population. The knowledge gained from these projects will enhance our understanding of the molecular events connected with the pathological conditions and pathogenesis of various conditions that affect veterans'' health.

10倍基因组铬系统是我们研究的重要工具。 10倍 基因组铬系统已完成铬控制器铬i7多路复用 套件，铬单细胞3'库和凝胶珠套件，以及铬单细胞A芯片套件。 这些组件一起用于执行基因组“链接读取”库准备和 测序。这些组件只能与铬控制器一起使用，不能使用 独立。有许多研究人员正在研究特定的单细胞类型 阐明各种生物过程的作用机制。这在原代细胞中很难 因为有许多细胞类型必须彼此隔离。该系统可以识别 单个单元，因此可以研究不同的细胞群体，而没有其他细胞类型混淆 结果。这个单细胞和DNA分析10x Genomics™由10x Gemcode™组成 燃料系统具有创新的试剂递送以前无法访问的基因组 以巨大的速度和规模信息。铬控制器可以迅速封装 单个细胞或基因组DNA分为100,000至1,000,000 s唯一的可寻址分区 分钟，每个都包含一个识别条形码，用于下游基因组分析 铬软件套件。它将使我们能够探索衰老和其他疾病状况如何 影响主要类别的结构变体（SVS），例如缺失，反转和易位以及 还允许对短阅读测序仪无法访问的基因进行测序。 10x Genomics™Will 还允许在> 10 MB单倍型块上分析SNV，INDEL和SVS。最后，它 将允许从低至1 ng的基因组DNA产生高质量的文库。 这项技术对VA研究非常重要。有14个主要调查员 在Vapahcs，将在其研究中应用这项技术来分析基因表达 单细胞水平以阐明衰老，类固醇生成，伤口/骨愈合的机制 代谢，细胞免疫回报，T细胞激活，骨骼疾病，肥胖，海湾战争 疾病以及许多其他代谢性疾病和疾病特别影响 退伍军人人口。从这些项目中获得的知识将增强我们的理解 与各种病理条件和发病机理有关的分子事件的 影响退伍军人的健康状况。