ShEEP Request for Chromium System

ShEEP 对 Chromium 系统的请求

• 批准号: 9796800
• 负责人: Salman Azhar
• 金额: --
• 依托单位: VETERANS ADMIN PALO ALTO HEALTH CARE SYS
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9796800
• 关键词: Affect; Aging; Autoimmune Diseases; Biological; Biological Process; Biology; Blood Vessels; Bone Diseases; Cancer Model; Cells; Chromium; Computer software; DNA; DNA analysis; Data; Degenerative polyarthritis; Development; Disease; Disease model; Encapsulated; Event; Gel; Gene Expression; Generations; Genes; Genome; Genomic DNA; Genomics; Gulf War; Haplotypes; Health; Healthcare; Heterogeneity; Human; Human Genome; Immune; Immune response; Individual; Injury; Knowledge; Label; Libraries; Link; Metabolic Diseases; Metabolic syndrome; Mission; Molecular; Molecular Weight; Muscle; Nerve Degeneration; Obesity; Oils; Pain; Pathogenesis; Pathologic; Patient Care; Phase; Population; Preparation; Process; Radiation; Reagent; Research; Research Personnel; Rodent; Rotavirus Infections; Sampling; Sheep; Stem cells; Steroid biosynthesis; Structure; System; T cell regulation; T-Cell Activation; Technology; Variant; Veterans; Whole-Genome Shotgun Sequencing; Work; adipokines; artery stenosis; bone healing; cell type; design; exome; genome analysis; health administration; improved; innovation; insertion/deletion mutation; instrument; lipid metabolism; lipid transport; nanolitre; non-alcoholic fatty liver disease; research study; targeted treatment; wound

The 10x Genomics Chromium System is an important instrument for our research. The 10X Genomics Chromium System is comprised of the Chromium Controller, Chromium i7 Multiplex kit, Chromium Single Cell 3' Library and Gel Bead Kit, and the Chromium Single Cell A Chip kit. These components are used together to perform Genome “linked read” library preps and sequencing. These components will only work with the Chromium Controller and cannot be used independently. There are many investigators that are researching specific single cell types to elucidate mechanisms of action of various biological processes. This is hard to do in primary cells as there are many cell types that must be isolated from one another. This system can identify single cells, so different cell populations can be studied without other cell types confounding the results. This Single Cell and DNA Analysis 10X Genomics™ consists of 10x GemCode™ that fuels Chromium System with an innovative reagent delivery of previously inaccessible genomic information at a massive rate and scale. The Chromium Controller can quickly encapsulate individual cells or genomic DNA into 100,000s to 1,000,000s of uniquely addressable partitions in minutes, each containing an identifying barcode for downstream genomic analysis with the Chromium Software Suite. It will allow us to explore how aging and other diseases conditions impact major classes of structural variants (SVs) like deletions, inversions and translocations, and also allows the sequencing of genes inaccessible to short-read sequencers. 10X Genomics™ will also allow analysis of Phase SNVs, indels and SVs across > 10 Mb haplotype blocks. Finally, it will allow generation of high-quality libraries from as little as 1 ng of genomic DNA. This technology is extremely important to VA research. There are fourteen principle investigators at VAPAHCS who will apply this technology in their research to analyze gene expression at a single cell level to elucidate mechanisms of aging, steroidogenesis, wound/bone healing, lipid metabolism, cellular immune responses, T cell activation, bone diseases, obesity, Gulf War Illness, as well as many other metabolic diseases and conditions that particularly affect the Veterans population. The knowledge gained from these projects will enhance our understanding of the molecular events connected with the pathological conditions and pathogenesis of various conditions that affect veterans'' health.

10x Genomics Chromium 系统是我们研究的重要仪器。 Genomics Chromium 系统由 Chromium 控制器、Chromium i7 Multiplex 组成 试剂盒、Chromium Single Cell 3' Library 和 Gel Bead 试剂盒以及 Chromium Single Cell A Chip 试剂盒。 这些组件一起用于执行基因组“链接读取”文库准备和 这些组件只能与 Chromium 控制器配合使用，不能使用。 有许多研究人员正在独立研究特定的单细胞类型。 阐明各种生物过程的作用机制在原代细胞中很难做到。 因为该系统可以识别多种细胞类型。 单细胞，因此可以研究不同的细胞群，而不会与其他细胞类型混淆 该单细胞和 DNA 分析 10X Genomics™ 由 10x GemCode™ 组成， 通过创新的试剂输送以前无法访问的基因组为 Chromium 系统提供燃料 Chromium 控制器可以快速封装大量信息。 将单个细胞或基因组 DNA 分成 100,000 到 1,000,000 个独特的可寻址分区 分钟，每个包含一个识别条形码，用于下游基因组分析 Chromium 软件套件将使我们能够探索衰老和其他疾病的状况。 结构变异（SV）的主要影响类别，如删除、倒置和易位，以及 还可以对 10X Genomics™ 无法访问的基因进行测序。 还允许分析 > 10 Mb 单倍型块的阶段 SNV、插入缺失和 SV。 将允许从低至 1 ng 的基因组 DNA 生成高质量的文库。 这项技术对于 VA 研究极其重要，有 14 名主要研究者。 在VAPAHCS，他们将在他们的研究中应用这项技术来分析基因表达 单细胞水平阐明衰老、类固醇生成、伤口/骨愈合、脂质的机制 新陈代谢、细胞免疫反应、T 细胞激活、骨病、肥胖、海湾战争 疾病以及许多其他代谢疾病和状况特别影响 退伍军人从这些项目中获得的知识将增强我们的理解。 与各种病理状况和发病机制相关的分子事件 影响退伍军人健康的条件。