ShEEP request for an Inscopix nVoke Integrated Imaging and Optogenetics System

ShEEP 请求 Inscopix nVoke 集成成像和光遗传学系统

• 批准号: 9795729
• 负责人: Karunesh Ganguly
• 金额: --
• 依托单位: VETERANS AFFAIRS MED CTR SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9795729
• 关键词: Animals; Behavior; Behavioral; Brain; Cells; Code; Computer software; Contracts; Corpus striatum structure; Data; Development; Dissection; Environment; Equipment; Floor; Head; Hippocampus (Brain); Image; Implant; Implantation procedure; Individual; Injury; Intervention; Investigation; Knowledge; Label; Learning; Location; Maintenance; Memory; Microscope; Monitor; Nervous system structure; Neurofibrillary Tangles; Neurons; Pattern; Phase; Process; Recovery; Research; Resolution; Route; Sheep; Signal Transduction; Stroke; Structure; Survivors; System; Technology; Therapeutic Intervention; Time; Traumatic Brain Injury; United States; Veterans; Work; awake; cell type; cost; data acquisition; disability; equipment acquisition; flexibility; insight; lens; neurotransmission; new technology; novel therapeutics; optogenetics; relating to nervous system

Stroke and traumatic brain injury are leading causes of disability among veterans. While there has been substantial research into understanding mechanisms of recovery, there is a relative dearth of studies that delineate the contributions of specific cell types, e.g. excitatory and/or inhibitory subtypes. Such knowledge provides greater insight into the recovery process and can aid the development of targeted interventions. We request the purchase of the Inscopix nVoke Integrated Imaging and Optogenetics System to conduct such investigations. The Inscopix miniature microscope platform gives us an edge in investigating how patterned activity in neuronal ensembles represents information about an individual’s environment, actions, and memories. A key advantage of this system is the ability to image deep neural structures (e.g. striatum, hippocampus) through a flexible scope during freely moving behaviors. Importantly, by monitoring and disrupting activity in the same cells over months, we can gain unprecedented insights into how neural codes evolve during recovery and with therapeutic interventions. A critical aspect of this technology is unprecedented monitoring at a single-cell resolution of identified cell-types; this represents the critical next phase of circuit function dissection.

中风和创伤性脑损伤是退伍军人残疾的主要原因。 对理解恢复机制进行了大量研究，有相对深入的研究 描述特定细胞类型的贡献，例如兴奋性和/或抑制性亚型。 知识可以让您更深入地了解恢复过程，并有助于制定有针对性的治疗方案 我们请求购买 Inscopix nVoke 集成成像和光遗传学。 Inscopix 微型显微镜平台为我们提供了进行此类研究的优势。 研究神经系统中的模式活动如何代表个体的信息 该系统的一个关键优势是能够对深度神经进行成像。 在自由移动行为过程中，通过灵活的范围来观察结构（例如纹状体、海马体）。 重要的是，通过数月监测和破坏相同细胞的活动，我们可以获得 对神经编码在恢复期间和治疗干预过程中如何演变的前所未有的见解。 该技术的一个关键方面是以单细胞分辨率进行前所未有的监测 细胞类型；这代表了电路功能解剖的关键下一阶段。