ShEEP Request for 4D Bioprinting-Biofabrication of stimuli-responsive materials

ShEEP 请求 4D 生物打印-刺激响应材料的生物制造

• 批准号: 9795834
• 负责人: MARK S. KINDY
• 金额: --
• 依托单位: JAMES A. HALEY VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9795834
• 关键词: 3-Dimensional; Age; Alzheimer' s Disease; Animals; Biocompatible Materials; Biological Process; Biotechnology; Cells; Characteristics; Development; Disease; Drug usage; Goals; Head; Heart Diseases; Image; Implant; In Vitro; Individual; Injury; Link; Location; Magnetic Resonance Imaging; Malignant Neoplasms; Medical center; Organ; Organoids; Parkinson Disease; Pathologic; Pathologic Processes; Pharmaceutical Preparations; Printing; Process; Property; Reagent; Regenerative Medicine; Research; Research Personnel; Research Proposals; Sheep; Signal Transduction; Specific qualifier value; Stimulus; Stroke; Structure; Techniques; Testing; Time; Tissues; Traumatic Brain Injury; X-Ray Computed Tomography; biofabrication; bioprinting; cell growth; drug discovery; drug efficacy; efficacy testing; implantation; injured; instrument; regenerative; repaired; tissue regeneration

The goal of this proposal is to purchase a 3D bioprinter for the James A. Haley (JAH) VA Medical Center in Tampa, FL to help enhance the research efforts in biotechnology. 3D bioprinting is an evolving process that utilizes 3D printing techniques to combine cells, growth or trophic factors and biomaterials to fabricate organoids (small organ-like entities) or tissues that mimic the naturally occurring characteristics of a tissue or organ. The process can utilize multiple printing heads and a layer-by-layer process to place materials (bioinks, biomaterials) to generate the tissue-like structures that can be used to study developmental, signaling and pathological processes in the specified organ. The bioprinting of tissues or organoids can be used to test the impact of drugs on specific tissues, study the pathological effects of disease or injury in vitro to limit the use of animals in studies, and for regenerative medicine purposes. The process involves programming the instrument, or using magnetic resonance imaging (MRI) or computed tomography (CT) images to print the tissue. Next, the tissue is printed with cells, matrix and factors in specific locations to allow for the tissue to mimic a natural structure. Finally, the tissues can be used to study various parameters, including time or age (4D) on tissue development. To maintain our competitive edge at the JAH VAMC, the acquisition of the Izumi Bioprinter will allow the investigators to print tissues for studying Alzheimer’s disease (AD), Parkinson’s disease (PD), traumatic brain injury (TBI), stroke, cancer, heart disease, and other afflictions. In addition, the tissues can be used for drug discovery, and to study the regenerative properties of tissues and even to potentially be used as cassettes to implant into the organ for repair processes. The 3D bioprinter will be used to: 1) generate tissues and organoids to study biological and pathological processes; 2) test the efficacy of drugs and reagents that may alter the disease process; and 3) identify signaling mechanisms associated with tissue regeneration and tissue implantation to repair injured tissues. Also, the 3D bioprinter will help with the development of additional research proposals, both individual and linked MERITs to BLR&D.

该提案的目标是为 James A. Haley (JAH) VA 医疗中心购买一台 3D 生物打印机 佛罗里达州坦帕市旨在帮助加强生物技术的研究工作，3D 生物打印是一个不断发展的过程。 利用 3D 打印技术将细胞、生长或营养因子和生物材料结合起来制造类器官 （类似小器官的实体）或模仿组织或器官自然发生特征的组织。 工艺可以利用多个打印头和逐层工艺来放置材料（生物墨水、生物材料） 生成可用于研究发育、信号传导和病理的组织样结构 组织或类器官的生物打印可用于测试药物的影响。 针对特定组织，在体外研究疾病或损伤的病理影响，以限制在研究中使用动物， 对于再生医学目的，该过程涉及对仪器进行编程或使用磁性。 用于打印组织的磁共振成像 (MRI) 或计算机断层扫描 (CT) 图像 接下来，打印组织。 将细胞、基质和因子置于特定位置，使组织能够模仿自然结构。 组织可用于研究各种参数，包括时间或年龄（4D）对组织发育的维持。 我们在 JAH VAMC 的竞争优势，收购 Izumi Bioprinter 将使研究人员能够打印 用于研究阿尔茨海默病 (AD)、帕金森病 (PD)、创伤性脑损伤 (TBI)、中风、 此外，这些组织还可用于药物发现和研究。 组织的再生特性，甚至有可能用作盒式植入器官 3D 生物打印机将用于：1）生成组织和类器官以研究生物和生物。 病理过程；2) 测试可能改变疾病过程的药物和试剂的功效；3) 识别与组织再生和组织植入修复损伤相关的信号机制 此外，3D 生物打印机将有助于制定额外的研究计划，无论是个人还是个人。 并将 MERIT 与 BLR&D 联系起来。