Nanotechnology Strategies for Growth of Bones and Teeth

骨骼和牙齿生长的纳米技术策略

基本信息

批准号：
7245070
负责人：
SAMUEL I STUPP
金额：
$ 58.27万
依托单位：
NORTHWESTERN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-07-12 至 2010-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7245070
关键词：
Affect Allografting Ameloblasts Autologous Binding Proteins Biological Biological Assay Biology Biomedical Research Biomimetics Bone Growth Bone Matrix Bone Regeneration Bone Tissue Cell Adhesion Cells Cephalic Chemistry Defect Dental Dental Enamel Development Embryo Encapsulated Extracellular Space Gene Expression Growth Factor Habits Healed Implant In Vitro Integrin Binding Laboratories Liquid substance Modeling Mus Nanostructures Nanotechnology Natural regeneration Organ Culture Techniques Orthopedic Surgery procedures Osteogenesis Pattern Peptides Performance Physiological Production Proteins Rattus Recombinant Proteins Research Research Personnel Signal Transduction Site Standards of Weights and Measures Stem cells System Testing Tissue Transplantation Tissues Tooth structure base biomineralization bone design healing in vitro Assay in vitro Model in vivo in vivo regeneration interest mineralization nanofiber nanolithography nanoscale nanoscience nanostructured performance tests programs repaired scaffold self assembly

项目摘要

DESCRIPTION (provided by applicant): This application proposes to develop molecularly designed peptide nanostructures that can trigger differentiation of progenitor cells and mineralization in physiological media with features that mimic enamel or bone matrices. The proposed research is based on the hypothesis that regeneration of enamel and bone can be triggered by templates designed at the nanoscale to present biological signals and induce biomimetic mineralization. Using in vitro assays, we will investigate how these biomimetic mineralization templates affect matrix synthesis by ameloblasts and osteoprogenitor cells, and also test their performance in embryonic tooth culture and in vivo bone repair models. This research will guide the development of synthetic, versatile, nanostructured templates that can be used for hard tissue (enamel or bone) regeneration and repair without the need for autologous or autogenic tissue transplantation. The proposed program's specific aims include: development of peptide amphiphiles that will self-assemble in physiological fluids and trigger biomimetic mineralization of enamel- or bone-like matrices, testing of these nanofiber systems in vitro with osteoprogenitor and ameloblast cells using mineralization, protein and gene expression assays, and using patterned substrates or scaffolds of peptide amphiphile nanofibers for in vitro and in vivo regeneration of dental and bone tissues in murine models. The proposed research program is a collaborative effort of four investigators specializing in self-assembly/materials chemistry, dental tissue biology and orthopedic surgery, whose interests intersect on the focused application of nanoscience and nanotechnology to biomedical research.

描述（由申请人提供）：本申请建议开发分子设计的肽纳米结构，这些肽纳米结构可以触发祖细胞的分化和生理培养基的矿化，并具有模仿搪瓷或骨基质的特征。拟议的研究基于以下假设：在纳米级设计的模板可以触发搪瓷和骨骼的再生，以呈现生物学信号并诱导仿生矿化。使用体外测定法，我们将研究这些仿生矿化模板如何影响成成成成成组织和成骨构细胞的基质合成，并测试其在胚胎牙齿培养和体内骨修复模型中的性能。这项研究将指导可用于硬组织（搪瓷或骨骼）再生和修复的合成，多功能，纳米结构的模板的发展，而无需自体或自源组织移植。拟议的计划的具体目的包括：肽两亲物的开发，这些肽将在生理流体中自组装并触发牙釉质或骨状基质的生物模仿矿化，这些纳米纤维系统与成骨纤维中的纳米纤维系统在成骨纤维中测试成骨蛋白酶，并使用矿物质和蛋白质的蛋白质和蛋白质的分类，或使用模式的蛋白质，或使用模式的蛋白质，或使用模式的蛋白质，或使用模式的蛋白质，或使用模式化的蛋白质，或者使用模式。鼠模型中牙科和骨组织的体外和体内再生的纳米纤维。拟议的研究计划是四名专门从事自组装/材料化学，牙科组织生物学和骨科手术的研究人员的协作工作，他们的兴趣与集中应用纳米科学和纳米技术在生物医学研究中相关。