Harnessing PET to Study the In Vivo Fate and Health Effects of Micro- and Nanoplastics

利用 PET 研究微塑料和纳米塑料的体内命运和健康影响

基本信息

批准号：
10890903
负责人：
Outi Maria Keinaenen
金额：
$ 24.9万
依托单位：
UNIVERSITY OF ALABAMA AT BIRMINGHAM
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-15 至 2026-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10890903
关键词：
Air Animals Autoradiography Behavior Biodistribution Biological Biological Markers Biological Models Cardiovascular system Chelating Agents Chronic Communication Consumption Data Decision Making Deposition Disease Dose Employment Environment Environmental Pollutants Environmental Pollution Evaluation Exposure to Fetal Development Food Foundations Fresh Water Future Goals Grant Health Histology Human Image Imaging Techniques Immunohistochemistry In Vitro Inflammation Inflammatory Inflammatory Response Ingestion Inhalation Interdisciplinary Study Intestines Investigation Iodine Radioisotopes Label Laboratories Light Liquid substance Lung Mammals Mentors Methods Microscopic Modeling Mus Musculoskeletal System Nervous System Nulliparity Oral Outcome Particle Size Pharmacology Study Pharmacology and Toxicology Phase Physiological Placenta Plastics Polystyrenes Positron-Emission Tomography Postdoctoral Fellow Pregnancy Process Proliferating Radiochemistry Radiolabeled Radiopharmaceuticals Research Resources Role Route Seawater Sewage Shapes Site Soil Stomach Structure Surface Techniques Time Tissues Toxic effect Tracer Visualization Work absorption bioaccumulation career career development collaborative environment college drinking water experimental study exposed human population exposure route imaging approach in vivo in vivo evaluation innovation meter molecular imaging nuclear imaging particle pollutant pre-clinical pregnant programs radiotracer remediation research and development sialic acid binding Ig-like lectin skills tool trend uptake

项目摘要

ABSTRACT CANDIDATE: The candidate is a postdoctoral fellow in the laboratory of Dr. Zeglis at Hunter College of CUNY. Her proposed research will provide a strong foundation for independent research career following the K99 phase of this grant. Her long-term career goal is to establish a robust program in environmental pollution research utilizing molecular imaging. To achieve this, she has developed a career plan that will (1) broaden her technical and scientific skills (2) strengthen her presentation and communication skills, and cultivate her professional relationships, (3) enrich her mentoring skills, and (4) prepare her for the transition to independence. RESEARCH: The proliferation of micro- and nanoplastics in the environment is increasing at an alarming rate. Micro- and nanoplastics have been found to be persistent and ubiquitous pollutants in a variety of environments, including sea water, fresh water, soil, sewage, and air. In light of this harrowing phenomenon, the evaluation of the possible health effects of microplastic (<5 mm) and nanoplastic (<100 nm) pollutants has emerged as an unmet scientific need. The over-arching goal of this investigation is to study the biodistribution and health effects of micro- and nanoplastics in mammals using a sensitive and quantitative molecular imaging technique: positron emission tomography (PET). The exquisite sensitivity of PET is major advantage of this strategy, as it will facilitate the non-invasive tracking of micro- and nanoplastics at environmentally relevant concentrations. The specific aims are: 1) To determine the bioaccumulation and bioretention of radioplastics in mice after pulmonary and oral exposure, 2) To quantify the bioaccumulation and bioretention of radioplastics in pregnant mice after oral and pulmonary exposure, and 3) To quantify the pro-inflammatory effects of micro- and nanoplastic exposure via non-invasive approaches. ENVIRONMENT: Hunter College is known for its highly collaborative environment to facilitate interdisciplinary research efforts such as those proposed in this application. The candidate is mentored by Dr. Zeglis, a recognized inorganic radiochemist with groundbreaking work in preclinical investigations of radiopharmaceuticals. In addition, she is co-mentored by Dr. Stapleton, an environmental toxicologist who has done pioneering and innovative contributions to the plastic research. Hunter College provides a rich set of collaborative, technical and scientific resources to execute the research and career development proposed here.

抽象的候选人：该候选人是纽约市立大学亨特学院 Zeglis 博士实验室的博士后研究员。她提出的研究将为 K99 阶段后的独立研究生涯奠定坚实的基础这笔补助金。她的长期职业目标是建立一个强有力的环境污染研究计划利用分子成像。为了实现这一目标，她制定了一项职业计划，该计划将 (1) 拓宽她的技术领域和科学技能（2）加强她的表达和沟通能力，培养她的专业能力人际关系，(3) 丰富她的指导技能，(4) 为她过渡到独立做好准备。研究：环境中微塑料和纳米塑料的扩散正以惊人的速度增加。微塑料和纳米塑料已被发现是各种环境中持久且普遍存在的污染物，包括海水、淡水、土壤、污水和空气。鉴于这一令人痛心的现象，评估微塑料（<5 毫米）和纳米塑料（<100 纳米）污染物可能对健康产生的影响已成为未满足的科学需求。这项调查的首要目标是研究生物分布和健康影响使用灵敏的定量分子成像技术：正电子对哺乳动物体内的微米和纳米塑料进行研究发射断层扫描（PET）。 PET 的高灵敏度是该策略的主要优势，因为它将促进对环境相关浓度的微塑料和纳米塑料进行非侵入性追踪。这具体目标是： 1) 确定放射性塑料在肺部注射后在小鼠体内的生物蓄积和生物滞留。和口服暴露，2) 量化怀孕小鼠体内放射性塑料的生物蓄积和生物滞留口腔和肺部暴露，以及 3) 量化微米和纳米塑料暴露的促炎作用通过非侵入性方法。环境：亨特学院以其高度协作的环境而闻名，以促进跨学科的发展研究工作，例如本申请中提出的研究工作。候选人由 Zeglis 博士指导，他是一位公认的无机放射化学家，在临床前研究方面具有开创性的工作放射性药物。此外，她还得到环境毒理学家 Stapleton 博士的共同指导。对塑料研究做出了开拓性和创新性的贡献。亨特学院提供了丰富的用于执行此处提出的研究和职业发展的协作、技术和科学资源。