CONSTRUCTION OF A FLEXIBLE HYPERPOLARIZATION SYSTEM

柔性超极化系统的构建

• 批准号: 8363905
• 负责人: MATTHEW E MERRITT
• 金额: $ 6.43万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363905
• 关键词: 2,4-Dinitrophenol; Cell Nucleus; Chemicals; Collaborations; Detection; Eye; Frequencies; Funding; Grant; Journals; Magnetic Resonance; Mammals; Metabolism; Mind; National Center for Research Resources; Nuclear; Physics; Physiologic pulse; Principal Investigator; Protons; Published Comment; Pyruvic Acid; Research; Research Infrastructure; Resources; Sampling; Source; System; United States National Institutes of Health; Universities; Washington; base; cost; cryostat; design; flexibility; in vivo; instrument; magnetic field; research study; solid state nuclear magnetic resonance

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A flexible dynamic nuclear polarization instrument is under construction at the RR. It is planned to be a more flexible instrument than the commercially-available Oxford HyperSense DNP system. Further, the terms and conditions of purchase of the HyperSense prohibit in vivo experiments. With an eye towards potential large mammal in vivo applications, the system is being designed around a larger sample container that will hold approximately twice the amount of sample as the HyperSense sample cup. The DNP system will be based on a cryostat designed in collaboration with Mark Conradi of Washington University placed in a Bruker 15cm bore magnet energized to a field of ~4.6 Tesla. Recent results have demonstrated that incremental increases in field strength can yield dramatically higher nuclear polarizations.(1,2) With this in mind, we have designed the new system to operate at an ESR frequency of ~129 GHz. Furthermore, the extra space available in the large bore magnet should allow the incorporation of a double resonance probe for 1H and either 13C or 89Y. Cross-polarization solid state NMR pulse sequences can then be used to transfer polarization from high gamma protons, which polarize much more quickly under DNP than lower gamma species, to the detection nucleus.(3) References for construction of a polarizer: 1. Jannin S, Comment A, Kurdzesau F, Konter JA, Hautle P, van den Brandt B, van der Klink JJ. A 140 GHz prepolarizer for dissolution dynamic nuclear polarization. The Journal of Chemical Physics 2008;128(24):241102-241104. 2. J¿hannesson H, Macholl S, Ardenkjaer-Larsen JH. Dynamic Nuclear Polarization of [1-13C]pyruvic acid at 4.6 tesla. Journal of Magnetic Resonance 2009;197(2):167-175. 3. Maly T, Debelouchina GT, Bajaj VS, Kan-Nian H, Chan-Gyu J, Mak-Jurkauskas ML, Sirigiri JR, van der Wel PCA, Herzfeld J, Temkin RJ, Griffin RG. Dynamic nuclear polarization at high magnetic fields. Journal of Chemical Physics 2008;128(5):N.PAG.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 RR正在建造一种柔性动态核极化仪器。它计划是比商业上可用的牛津超义DNP系统更灵活的工具。此外，在体内实验禁止购买高血压的条款和条件。为了注视潜在的大型哺乳动物在体内应用中，该系统是在较大的样品容器周围设计的，该容器将容纳大约两倍的样品量作为超义样品杯。 DNP系统将基于与华盛顿大学的马克·康拉迪（Mark Conradi）合作设计的低温恒温器。最近的结果表明，场强的增量增加可以动态地产生更高的核极性。（1,2）考虑到这一点，我们设计了新系统以〜129 GHz的ESR频率运行。此外，大孔磁铁中可用的额外空间应允许将双共振探针掺入1H和13C或89Y。然后，可以使用跨极化固态NMR脉冲序列将极化从高γ质子转移到DNP下的极化速度比低伽马种的偏振质量更快地转移到较低的伽马物种中。（3）构造偏振器的引用：1。Jannins，jannin s，ryment a，评论A，评论A，评论140 GHz预校正剂，用于溶解动态核极化。 《化学物理学杂志》 2008; 128（24）：241102-241104。 2。J¿HannessonH，Macholl S，Ardenkjaer-Larsen JH。 [1-13c]丙酮酸在4.6特斯拉处的动态核极化。磁共振杂志2009; 197（2）：167-175。 3。MalyT，Debelouchina GT，Bajaj VS，Kan-Nian H，Chan-Gyu J，Mak-Jurkauskas ML，Sirigiri JR，Van der Wel PCA，Herzfeld J，Temkin J，Temkin RJ，Griffin RG。高磁场的动态核极化。化学物理学杂志2008; 128（5）：N.PAG。