Core B: Tissue Specimen Core

核心 B：组织样本核心

基本信息

批准号：
10689278
负责人：
Stephanie Halene
金额：
$ 32.43万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-30 至 2026-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10689278
关键词：
Age Aging Aliquot Antigens Biological Biological Markers Biomedical Research Blood Blood Cells Blood specimen Bone Marrow Bone Tissue Catalogs Cell Aging Cell Cycle Arrest Cell Maturation Cells Clinical Collaborations Collection Communities Data Data Analyses Data Science Databases Environment Erythrocytes Excision Gender Goals Health Health Insurance Portability and Accountability Act Hematology Hematopoiesis Hematopoietic System Heterogeneity Histologic Homeostasis Human Human body Immune Immunobiology Individual Individual Differences Infrastructure Invaded Kidney Knowledge Laboratories Link Liver Longevity Lung Lymphoid Tissue Maintenance Maps Medicine Metadata Molecular Motivation Multiomic Data Natural regeneration Operative Surgical Procedures Organ Organism Particulate Pathologic Pathology Patients Pharmaceutical Preparations Pharyngeal structure Phenotype Physiological Physiology Play Preparation Process Protocols documentation Race Research Research Personnel Role Sampling Specimen Spleen Stream Stress Structure Subjects Selections System T-Lymphocyte Thymus Gland Tissue Banks Tissue Sample Tissues Tonsil Training United States National Institutes of Health adaptive immune response age related aged clinical care comorbidity functional disability health data hip replacement arthroplasty human tissue immune function improved insight interest lymph nodes lymphoid organ microorganism multiple omics participant enrollment particle primary lymphoid organ secondary lymphoid organ senescence synergism tissue mapping wound healing

项目摘要

PROJECT SUMMARY – Core B (Biospecimen Core) Primary (bone marrow and thymus) and secondary (tonsil, lymph node, spleen and others) lymphoid organs are among the most dynamic tissues throughout an individual’s lifetime. The bone marrow gives rise to hundreds of billions of cells each day, the thymus educates T-cells to recognize self and non-self and tonsils, lymph nodes, spleen and others are key to daily defense against invading organisms and maintenance of cellular homeostasis. Cellular senescence in these lymphoid tissues have been observed and presumably associated with stress-induced or age-related functional impairment. However, what types or subtypes of senescent cells are present and their spatial heterogeneity in in the tissue context as well as how these cells impact the tissue environments remain poorly understood, highlighting a pressing need to map senescent cells and the surround environments in these tissues and generate biomarkers to define spatial and phenotypic heterogeneity in cellular senescence. The Biospecimen Core will fulfill a critical role in the Yale SenNet TMC to procure, process, and histologically annotate lymphoid tissue specimens for further analysis in the Biological Analysis Core to generate molecular and cellular maps of senescent cells. It will leverage and significantly expand upon the Yale Hematology Tissue Bank’s existing structure and further leverage the existing collaborations between Hematology (Dr. Halene), Pathology (Dr. Xu), Immunobiology (Dr. Craft), Laboratory Medicine and YNHH Health Data Science (Dr. Schulz), and Surgery (Dr. Kodadek) to collect lymphoid tissue and concurrent and longitudinal multi-specimen blood samples from healthy individuals. The Biospecimen Core will collect, process, aliquot, catalog, annotate high quality tissue samples and distribute these to the Yale SenNET Biological Analysis Core and SenNET investigators. The Biospecimen Core will leverage its existing Freezerworks database to integrate all data from patient to tissue aliquot and link this data to clinical and research databases in a HIPAA compliant and auditable fashion. The tissue specimens including tissue sections and biofluids are further analyzed by the Biological Analysis Core and the specimen metadata will be managed in the Data Analysis Core for integrated analysis and sharing.

项目摘要 – 核心 B（生物样本核心）初级（骨髓和胸腺）和次级（扁桃体、淋巴结、脾脏等）淋巴器官骨髓是人一生中最具活力的组织之一。每天有数千亿个细胞，胸腺训练 T 细胞识别自我、非自我和扁桃体，淋巴结、脾脏和其他器官是日常防御入侵生物和维持免疫系统的关键。已观察到并推测这些淋巴组织中的细胞衰老。然而，哪些类型或亚型与压力引起的或与年龄相关的功能障碍有关。衰老细胞的存在及其在组织环境中的空间异质性以及这些细胞如何对组织环境的影响仍然知之甚少，这凸显了绘制衰老细胞图谱的迫切需要和这些组织中的周围环境，并生成生物标志物来定义空间和表型 Biospecimen Core 将在耶鲁 SenNet TMC 中发挥关键作用，以实现细胞衰老的异质性。获取、处理和组织学注释淋巴组织样本，以便在生物实验室中进行进一步分析分析核心生成衰老细胞的分子和细胞图谱，它将显着利用并显着。扩展耶鲁大学血液学组织库的现有结构，并进一步利用现有的血液学（Halene 博士）、病理学（徐博士）、免疫生物学（Craft 博士）、实验室之间的合作医学和 YNHH 健康数据科学（Schulz 博士）和外科手术（Kodadek 博士）收集淋巴组织以及来自健康个体的同时和纵向多样本血液样本。 Core 将收集、处理、等分、编目、注释高质量组织样本并将其分发给耶鲁大学 SenNET 生物分析核心和 SenNET 研究人员将利用其现有的能力。 Freezerworks 数据库整合从患者到组织等份的所有数据，并将这些数据链接到临床和以符合 HIPAA 且可审核的方式建立研究数据库组织样本（包括组织）。切片和生物体液由生物分析核心进一步分析，样本元数据将被在数据分析核心中进行管理，以进行集成分析和共享。