Disruption of Host-Seeking Behavior in Aedes and Culex Mosquitoes using Odorants

使用气味剂破坏伊蚊和库蚊的宿主寻找行为

• 批准号: 8014899
• 负责人: Anandasankar Ray
• 金额: $ 35.83万
• 依托单位: UNIVERSITY OF CALIFORNIA RIVERSIDE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-01 至 2015-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8014899
• 关键词: Aedes; Agonist; Air; Behavior; Behavioral; Biological Assay; Blood; Carbon Dioxide; Chemicals; Cues; Culex (Genus); Culicidae; Dengue; Detection; Development; Disease; Electrophysiology (science); Exhalation; Family; Female; Filaria bancrofti; Filarial Elephantiases; Generations; Goals; Human; Insect Repellents; Insecta; Lead; Ligands; Masks; Mediating; Methods; Modification; Molecular Genetics; Neurons; Odors; Parasites; Physiological; Play; Research; Role; Skin; Smell Perception; System; Testing; West Nile virus; Yellow fever virus; carbon dioxide receptor; cheminformatics; feeding; improved; in vivo; inhibitor/antagonist; member; neurophysiology; novel; programs; public health relevance; receptor; receptor function; response; vector; vector control

DESCRIPTION (provided by applicant): Aedes aegypti mosquitoes transmit Dengue and Yellow Fever virus, and Culex quinquefasciatus mosquitoes transmit the West Nile virus and filarial parasite Wuchereria bancrofti to hundreds of millions of people. Methods for inhibiting vector- human contact, particularly in conjunction with other existing programs of vector control, may play a critical role in controlling the spread of these diseases. Female mosquitoes, like most other blood-feeding insects, identify their human hosts primarily using olfactory cues like carbon dioxide in exhaled breath, as well as other odors present in skin emanations. The goal of this research is to modify odor-guided host-seeking behavior of Aedes and Culex mosquitoes using cheap and environmentally safe odor molecules that can be used in very small quantities as effective trapping agents, masking agents and repellents. To achieve these goals we will focus on functional analyses of the CO2 sensing neuron. CO2 is detected in this neuron using a receptor comprising three members of the Gustatory receptor family that encode a highly conserved heteromeric CO2 receptor. We plan to use an integrated platform of neurophysiology, chemical- informatics, molecular, genetic and behavioral methods to; 1) identify effective volatile agonists and antagonists for the CO2 receptors, 2) test the effects of these molecules on behavior of female Aedes and Culex mosquitoes, in particular, for the ability of antagonists in masking agents or repellents, and 3) agonists to improve trapping efficiency. In preliminary studies, we have identified a few volatile inhibitors and activators of the CO2 receptor in Aedes and Culex. We now propose to conduct further studies to identify more effective chemicals that inhibit or activate physiological and behavioral responses to CO2 in female mosquitoes. Successful completion of the proposed studies will lead to the development of a new generation of effective and safe insect repellents and lures that act by modifying CO2 receptor function. PUBLIC HEALTH RELEVANCE: Aedes and Culex mosquitoes transmit several diseases including Dengue and lymphatic filariasis, and it is estimated that there are more than a hundred million people worldwide that are infected. Mosquitoes find their human hosts using the sense of smell. The goal of the proposed research is to modify odor-guided host-seeking behavior of Aedes and Culex mosquitoes using cheap and environmentally safe odor molecules that can be used in very small quantities as effective trapping agents, masking agents and repellents. Successful completion of the proposed studies will lead to the development of a new generation of effective and safe insect repellents and lures that act by modifying odor receptor function.

描述（由申请人提供）：埃及埃及蚊子将登革热和黄热病病毒传播，Culex Quinquefasciatus蚊子将西尼罗河病毒和丝状寄生虫bancrofti传播给数亿人。抑制媒介接触的方法，特别是与其他现有的矢量控制程序结合使用，可能在控制这些疾病的传播方面起着至关重要的作用。与大多数其他喂食昆虫一样，雌性蚊子主要使用嗅觉提示，例如呼出的呼吸中的二氧化碳，以及皮肤发出中存在的其他气味。这项研究的目的是使用廉价且环境安全的气味分子来修改埃德斯和库莱克斯蚊子的气味引导的宿主寻求行为，这些分子可以用作有效的捕获剂，掩盖剂和驱虫剂的有效捕获剂，较少的剂量。为了实现这些目标，我们将专注于CO2传感神经元的功能分析。在该神经元中检测到二氧化碳，该受体使用包含三个编码高度保守的异质二氧化碳受体的味觉受体家族的三个成员。我们计划使用神经生理学，化学信息学，分子，遗传和行为方法的集成平台； 1）确定二氧化碳受体的有效挥发激动剂和拮抗剂，2）测试这些分子对雌性埃德犬和库氏蚊子行为的影响，尤其是在掩盖剂或驱虫剂中拮抗剂的能力，以及3）激动剂提高捕获效率。在初步研究中，我们已经确定了艾edes和culex中二氧化碳受体的一些挥发性抑制剂和活化剂。现在，我们建议进行进一步的研究，以确定抑制或激活女性蚊子对CO2的生理和行为反应的更有效的化学物质。成功完成拟议的研究将导致新一代有效且安全的驱虫剂和诱饵通过修改CO2受体功能来发挥作用。 公共卫生相关性：迪士和库氏蚊子传播了包括登革热和淋巴丝虫病在内的几种疾病，据估计，全世界有超过一亿人被感染。蚊子使用嗅觉找到了人类的宿主。拟议的研究的目的是使用廉价且具有环境安全的气味分子来修改埃德斯和库尔克斯蚊子的气味引导的宿主寻求行为，这些分子可用于少量用作有效的诱捕剂，掩盖剂和驱虫剂。成功完成拟议的研究将导致新一代有效且安全的驱虫剂和诱饵通过修饰气味受体功能而起作用。