Cytapheresis Of Volunteer Donors (MRI 2003-054)

志愿者捐献者的细胞分离术 (MRI 2003-054)

• 批准号: 7969911
• 负责人: Dan Longo
• 金额: $ 43.7万
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7969911
• 关键词: Aging-Related Process; American; Blood Component Removal; Cell Count; Cells; Cellular Structures; Chronic; Collection; Complete Blood Count; Cytapheresis; HIV; Hepatitis; Hour; Human; Individual; Infection; Laboratories; Laboratory Study; Leukocytes; Liver; Lymphocyte; Magnetic Resonance Imaging; Medical; Molecular Genetics; National Institute on Aging; Participant; Procedures; Process; Protocols documentation; Qualifying; Recruitment Activity; Renal function; Research; Research Personnel; Sampling; Screening procedure; Testing; Urinalysis; base; expectation; immune function; meetings; monocyte; trend; volunteer

In this protocol, volunteers are recruited to undergo a two hour cytapheresis procedure for collection of large quantities of human circulating white blood cell components (lymphocytes or monocytes) from single donors for the use of National Institute on Aging laboratory investigators. Pheresis packs are supplied to individual qualified investigators as anonymous packs for use in basic studies of lymphocyte and monocyte function and studies of immune function in the aging process. Based on extensive modified American Bloodbank Association questionaires, laboratory screening including assessment of complete blood counts, urinalysis, liver and renal function as well as virologic screening including HIV and Hepatitis testing, the possibility of influence of extraneous infections or other chronic medical conditions on laboratory studies is minimized. In the course of the last year, the established donor pool has remained constant at approximately 70 participants with 221 procedures performed. Cell packs were supplied to individual investigators for research purposes. Based on trends over the last several years, there is an increasing need for cells by a range of laboratories with expectation for continued expansion of participants as well as procedures in this protocol in the next year.

在该方案中，招募志愿者进行两小时的细胞分离程序，从单个捐赠者处收集大量人类循环白细胞成分（淋巴细胞或单核细胞），供国家老龄化研究所实验室研究人员使用。分离包作为匿名包提供给合格的个人研究人员，用于淋巴细胞和单核细胞功能的基础研究以及衰老过程中免疫功能的研究。基于广泛修改的美国血库协会调查问卷，实验室筛查包括全血细胞计数评估、尿液分析、肝肾功能以及病毒学筛查，包括艾滋病毒和肝炎检测、外来感染或其他慢性疾病对实验室研究影响的可能性被最小化。去年，已建立的捐赠者库一直保持在大约 70 名参与者，进行了 221 项手术。 细胞包被提供给个别研究人员用于研究目的。根据过去几年的趋势，一系列实验室对细胞的需求不断增加，预计明年参与者以及本协议中的程序将继续扩大。